丝胶对STZ致损伤INS-1细胞活性和胰岛素分泌的影响

承德医学院学报 ›› 2017, Vol. 34 ›› Issue (3): 190-192.

丝胶对STZ致损伤INS-1细胞活性和胰岛素分泌的影响

王小杰¹, 刘美晓¹, 谢云鹏², 孙一婵¹, 陈志宏^1,△

1.承德医学院,河北承德 067000;
2.承德医学院附属医院

收稿日期:2016-08-20 出版日期:2017-06-10 发布日期:2021-11-26
通讯作者: ^△
基金资助:
*国家自然科学基金资助项目(81441133),河北省自然科学基金资助项目(H2013406096),河北省教育厅青年基金项目(QN20131089)

EFFECTS OF SERICIN ON CELL VIABILITY AND INSULIN SECRETION OF STZ INJURED INS-1 CELLS

Chengde Medical College, Hebei Chengde 067000, China

Received:2016-08-20 Online:2017-06-10 Published:2021-11-26

摘要/Abstract

摘要： 目的: 探讨丝胶对STZ致损伤的大鼠胰岛素瘤INS-1细胞活性和胰岛素分泌的影响。方法: 以链脲佐菌素（STZ）诱导INS-1细胞损伤,细胞分为正常组、STZ组和丝胶组(300μg/L)。普通光学倒置显微镜观察各组细胞的一般状态;MTT法测定细胞活性;ELISA法检测培养上清中胰岛素的含量。结果: 与正常组相比,STZ组细胞活性明显下降,培养上清中胰岛素的含量明显降低（P<0.05）。经300μg/L丝胶处理后,INS-1细胞活性、培养上清中胰岛素的含量均明显升高（P<0.05）。结论: 丝胶蛋白可提高STZ致损伤大鼠胰岛素瘤INS-1细胞的活性,以及促进胰岛素的分泌。

关键词: 丝胶, 链脲佐菌素（STZ）, INS-1细胞, 细胞活性, 胰岛素分泌

Abstract: Objective: To investigate the effects of sericin on cell viability and insulin secretion of streptozotocin (STZ)injured rats’ insulinoma INS-1 cells. Methods: The INS-1 cells were randomly divided into normal group, STZ group and sericin group. The cells in STZ group were cultured with STZ(10mmol/L) for 24h; the cells in sericin group were cultured with STZ(10mmol/L) and sericin (300μg/L) at the same time for 24h. The general state of INS-1 cells was observed by optical inverted microscope, MMT to detect the cell viability of INS-1 cells and ELISA to detect the insulin content in supernate. Results: Compared with normal group, the cell viability and insulin content in supernate of INS-1 cells decreased obviously (P<0.05); After treating with sericin (300μg/L) , the cell viability and insulin content in supernate of INS-1 cells increased obviously (P<0.05). Conclusions: Sericin can promote insulin secretion by enhancing the cell viability of STZ injured rats ’insulinoma INS-1 cells.

Key words: Sericin, Streptozotocin (STZ), INS-1 cells, Cell viability, Insulin secretion

中图分类号:

R587.1

王小杰, 刘美晓, 谢云鹏, 孙一婵, 陈志宏. 丝胶对STZ致损伤INS-1细胞活性和胰岛素分泌的影响[J]. 承德医学院学报, 2017, 34(3): 190-192.

WANG Xiao-jie, LIU Mei-xiao, XIE Yun-peng, et al. EFFECTS OF SERICIN ON CELL VIABILITY AND INSULIN SECRETION OF STZ INJURED INS-1 CELLS[J]. Journal of Chengde Medical University, 2017, 34(3): 190-192.

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