茵栀黄对结构性雄甾烷受体CAR介导CYP3A4的转录调节作用

承德医学院学报 ›› 2017, Vol. 34 ›› Issue (5): 361-363.

• 基础医学 • 下一篇

茵栀黄对结构性雄甾烷受体CAR介导CYP3A4的转录调节作用

李宝群¹, 毕红东^1,△, 郭娜娜¹, 庞泽华²

1.承德医学院药理学教研室,河北承德 067000;
2.承德医学院2011级中西医本科

收稿日期:2016-10-25 出版日期:2017-10-10 发布日期:2021-11-29
通讯作者: △

TRANSCRIPTIONAL REGULATION OF YINZHIHUANG ON CYP3A4 MEDIATED BY STRUCTURAL MALE STEROID RECEPTOR CAR

LI Bao-qun, BI Hong-dong, GUO Na-na, et al

Department of Pharmacology, Chengde Medical College, Hebei Chengde 067000, China

Received:2016-10-25 Online:2017-10-10 Published:2021-11-29

摘要/Abstract

摘要： 目的: 探讨茵栀黄对CYP3A4转录表达的影响及机制。方法: Chang Liver细胞中加入0.01、0.1和1μM茵栀黄分别作用24h、36h和48h,采用RT-PCR法和报告基因方法检测CYP3A4 mRNA的表达水平。结果: 不同浓度茵栀黄分别处理Chang Liver细胞24h和36h可明显升高CYP3A4 mRNA的表达水平（P<0.05）;转入CAR载体的Chang Liver细胞经不同浓度茵栀黄处理36h均可激活CYP3A4的转录表达。结论: 茵栀黄可能通过CAR介导增加Chang Liver细胞CYP3A4的转录表达。

关键词: 茵栀黄, 结构性雄甾烷受体CAR, CYP3A4, 报告基因, 转录表达

Abstract: Objective: To investigate the effects and mechanisms of Yinzhihuang on transcription of CYP3A4. Methods: The Chang Liver cells were cultured with 0.01μM, 0.1μM, 1μM Yinzhihuang respectively for 24h, 36h and 48h. The expression of CYP3A4 mRNA was detected by RT-PCR and dual-luciferase reporter assay. Results: The Chang Liver cells were cultured with Yinzhihuang of different concentration for 24h and 36h could obviously increase the expression of CYP3A4 mRNA (P<0.05). The Chang Liver cells that transfected CAR vector were cultured with different concentrations of Yinzhihuang for 36h could activate transcription of CYP3A4. Conclusions: Yinzhihuang may increase the transcriptional expression of CYP3A4 in Chang Liver cells mediated by CAR.

Key words: Yinzhihuang, Structural male steroid receptor CAR, CYP3A4, Reporter gene, Transcriptional expression

中图分类号:

R961

李宝群, 毕红东, 郭娜娜, 庞泽华. 茵栀黄对结构性雄甾烷受体CAR介导CYP3A4的转录调节作用[J]. 承德医学院学报, 2017, 34(5): 361-363.

LI Bao-qun, BI Hong-dong, GUO Na-na, et al. TRANSCRIPTIONAL REGULATION OF YINZHIHUANG ON CYP3A4 MEDIATED BY STRUCTURAL MALE STEROID RECEPTOR CAR[J]. Journal of Chengde Medical University, 2017, 34(5): 361-363.

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茵栀黄对结构性雄甾烷受体CAR介导CYP3A4的转录调节作用

TRANSCRIPTIONAL REGULATION OF YINZHIHUANG ON CYP3A4 MEDIATED BY STRUCTURAL MALE STEROID RECEPTOR CAR

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