向干扰ARTN对子宫内膜癌Ishikawa细胞增殖能力的影响

承德医学院学报 ›› 2017, Vol. 34 ›› Issue (5): 368-370.

向干扰ARTN对子宫内膜癌Ishikawa细胞增殖能力的影响

王晓华¹, 张玉娟, 周晓慧¹, 徐倩¹

1.承德医学院,河北承德 067000;
2.承德医学院附属医院

收稿日期:2016-12-03 出版日期:2017-10-10 发布日期:2021-11-29

EFFECTS OF TARGETED INTERFERENCE OF ARTN ON PROLIFERATION OF ENDOMETRIAL CANCER ISHIKAWA CELLS

WANG Xiao-hua, ZHANG Yu-juan, ZHOU Xiao-hui, et al

Chengde Medical College, Hebei Chengde 067000, China

Received:2016-12-03 Online:2017-10-10 Published:2021-11-29

摘要/Abstract

摘要： 目的: 通过RNA干扰技术靶向抑制ARTN基因在子宫内膜癌Ishikawa细胞中的表达,探讨抑制ARTN基因对Ishikawa细胞增殖能力的影响。方法: 体外培养子宫内膜癌Ishikawa细胞,以pGPU6/GFP/Neo为载体构建ARTN基因的短发夹RNA（pGPU6/GFP/Neo-ARTN shRNA）干扰质粒,利用脂质体法转染人子宫内膜癌Ishikawa细胞,荧光显微镜下观察转染效率,应用Western Blot法检测转染后细胞ARTN蛋白的表达情况,CCK法检测Ishikawa细胞的增殖活性。结果: pGPU6/GFP/Neo-ARTN shRNA干扰质粒成功转染Ishikawa细胞,转染效率为80%;转染后48h,干扰组Ishikawa细胞ARTN蛋白的表达水平明显低于阴性对照组和正常组P<0.05）;转染后48h、72h、96h,干扰组Ishikawa细胞的增殖活性明显低于阴性对照组和正常组（P<0.05）。结论: 靶向干扰ARTN基因可抑制子宫内膜癌Ishikawa细胞的增殖能力。

关键词: 子宫内膜癌, ARTN基因, RNA干扰, 增殖

Abstract: Objective: To investigate the effects of inhibiting ARTN gene by targeted interference of ARTN gene on proliferation of endometrial cancer Ishikawa cells. Methods: Short hairpin RNA (pGPU6/GFP/Neo-ARTN shRNA) interference plasmid of ARTN gene was constructed using pGPU6/GFP/Neo as carrier, and transfected into human endometrial cancer Ishikawa cells by liposome method. The transfection efficiency was observe under fluorescence microscope; Western Blot was used to detected ARTN protein expression in Ishikawa cells after transfection; CCK method to detect the proliferative activity of Ishikawa cells. Results: The pGPU6/GFP/Neo-ARTN shRNA interference plasmid was successfully transfected into Ishikawa cells and the transfection efficiency was 80%. 48h after transfection, the ARTN protein expression of Ishikawa cells in interference group was obviously lower than negative control group and normal group (P<0.05). 48h, 72h and 96h after transfection, the proliferative activity of Ishikawa cells in interference group was obviously lower than negative control group and normal group (P<0.05). Conclusions: Targeted interference of ARTN gene can inhibit the proliferative activity of endometrial cancer Ishikawa cells.

Key words: Endometrial cancer, ARTN gene, RNA interference, Proliferation

中图分类号:

R737.33

王晓华, 张玉娟, 周晓慧, 徐倩. 向干扰ARTN对子宫内膜癌Ishikawa细胞增殖能力的影响[J]. 承德医学院学报, 2017, 34(5): 368-370.

WANG Xiao-hua, ZHANG Yu-juan, ZHOU Xiao-hui, et al. EFFECTS OF TARGETED INTERFERENCE OF ARTN ON PROLIFERATION OF ENDOMETRIAL CANCER ISHIKAWA CELLS[J]. Journal of Chengde Medical University, 2017, 34(5): 368-370.

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