针刺对慢性脑缺血大鼠三叉神经脊束核神经元钙通道Cav1.3表达及凋亡的影响*

承德医学院学报 ›› 2018, Vol. 35 ›› Issue (2): 96-98.

针刺对慢性脑缺血大鼠三叉神经脊束核神经元钙通道Cav1.3表达及凋亡的影响^*

郭森¹, 魏萌¹, 杨松鹤¹, 乔跃兵^1,2

1.承德医学院人体解剖学教研室,河北承德 067000;
2.沧州医学高等专科学校

收稿日期:2017-06-20 出版日期:2018-04-10 发布日期:2021-11-21
基金资助:
* 河北省自然科学基金资助项目（H2013406115）

EFFECTS OF ACUPUNCTURE ON CALCIUM CHANNEL CAV1.3 EXPRESSION AND NEURONAL APOPTOSIS OF SPINAL NUCLEUS OF TRIGEMINAL NERVE OF CHRONIC CEREBRAL ISCHEMIA RATS

GUO Sen, WEI Meng, YANG Song-he, et al

Department of Human Anatomy, Chengde Medical College, Hebei Chengde 067000, China

Received:2017-06-20 Online:2018-04-10 Published:2021-11-21

摘要/Abstract

摘要： 目的: 探讨针刺对慢性脑缺血大鼠三叉神经脊束核神经元钙通道Cav1.3表达及凋亡的影响。方法: 雄性Wistar大鼠36只,随机分成针刺组、缺血对照组、假手术组,通过阻断左侧颈总动脉和双侧椎动脉的方法制作慢性脑缺血模型,针刺组大鼠电针刺激大鼠“四白”穴15天。采用免疫组织化学方法检测大鼠三叉神经脊束核钙通道Cav1.3蛋白的表达情况,TUNEL法检测神经元的凋亡情况,电镜下观察神经元超微结构的变化。结果: 针刺组大鼠三叉神经脊束核中钙通道Cav1.3蛋白表达明显高于缺血对照组,但仍低于假手术组（P<0.05）;神经元凋亡数量为缺血对照组>针刺组>假手术组（P<0.05）。电镜下,针刺组大鼠三叉神经脊束核神经元内可见有衣小泡出现。结论: 针刺大鼠“四白”穴能提高慢性脑缺血大鼠三叉神经脊束核神经元钙通道Cav1.3的表达,减少神经元凋亡。

关键词: 针刺, 慢性脑缺血, 三叉神经脊束核, L型钙通道

Abstract: Objective: To investigate the effects of acupuncture on calcium channel Cav1.3 expression and neuronal apoptosis of spinal nucleus of trigeminal nerve of chronic cerebral ischemia rats. Methods: 36 male Wistar rats were divided randomly into acupuncture group, ischemia group and sham operation group. The chronic cerebral ischemia rats' model was made by occluding the left common carotid artery and bilateral vertebral arteries; and then the rats in acupuncture group were acupunctured the “Sibai acupoints” for 15 days. Immunohistochemical stain was used to detect the calcium channel Cav1.3 expression in spinal nucleus of trigeminal nerve, TUNEL to detect apoptosis of neurons, electron microscope to observe the ultrastructure of neurons. Results: The calcium channel Cav1.3 protein expression in spinal nucleus of trigeminal nerve of rats in acupuncture group was obviously higher than ischemia group, but still lower than sham operation group(P<0.05). The apoptotic neurons number of three groups from high to low was ischemia group, acupuncture group and sham operation group (P<0.05). The coated vesicles could be found in neurons of rats in acupuncture group under electron microscope. Conclusions: Acupuncture of rats' “Sibai acupoints” can obviously up regulate the calcium channel Cav1.3 expression and decrease neuronal apoptosis in spinal nucleus of trigeminal nerve of chronic cerebral ischemia rats.

Key words: Acupuncture, Chronic cerebral ischemia, Spinal nucleus of trigeminal nerve, L-type calcium channel

中图分类号:

R743

郭森, 魏萌, 杨松鹤, 乔跃兵. 针刺对慢性脑缺血大鼠三叉神经脊束核神经元钙通道Cav1.3表达及凋亡的影响^*[J]. 承德医学院学报, 2018, 35(2): 96-98.

GUO Sen, WEI Meng, YANG Song-he, et al. EFFECTS OF ACUPUNCTURE ON CALCIUM CHANNEL CAV1.3 EXPRESSION AND NEURONAL APOPTOSIS OF SPINAL NUCLEUS OF TRIGEMINAL NERVE OF CHRONIC CEREBRAL ISCHEMIA RATS[J]. Journal of Chengde Medical University, 2018, 35(2): 96-98.