17-AAG对人绒毛膜癌JAR细胞MMP-2和MMP-9表达的调控作用*

承德医学院学报 ›› 2018, Vol. 35 ›› Issue (3): 186-189.

17-AAG对人绒毛膜癌JAR细胞MMP-2和MMP-9表达的调控作用^*

谷苗, 崔丽军, 刘百艺, 安国庆, 许倩^△

承德医学院,河北承德 067000

收稿日期:2017-09-01 出版日期:2018-06-10 发布日期:2021-11-21
通讯作者: △
基金资助:
* 河北省高校重点学科建设项目资助（冀教高【2013】4号）; 河北省计生委项目（2013-A13）;河北省教育厅项目（QN2016012）

REGULATORY EFFECTS OF 17-AAG ON MMP-2 AND MMP-9 EXPRESSION IN HUMAN CHORIOCARCINOMA JAR CELLS

Chengde Medical College, Hebei Chengde 067000, China

Received:2017-09-01 Online:2018-06-10 Published:2021-11-21

摘要/Abstract

摘要： 目的: 探讨热休克蛋白90（HSP90）抑制剂17-丙烯胺基-17-去甲氧基格尔德霉素（17-AAG）对人绒毛膜癌JAR细胞基质金属蛋白酶（MMP）-2、MMP-9蛋白表达的调控作用。方法: 体外培养人绒毛膜癌JAR细胞,以不同浓度的17-AAG作用JAR细胞,采用MTT法检测JAR细胞的增殖抑制率,Western blotting法检测MMP-2、MMP-9蛋白的表达情况。结果: MTT结果显示,17-AAG对JAR细胞生长具有明显的抑制作用,并呈浓度依赖性。5μg/ml、10μg/ml、20μg/ml、40μg/ml的17-AAG作用后,JAR细胞MMP-2、MMP-9蛋白的表达明显下调。结论: 17-AAG对人绒毛膜癌JAR细胞的增殖具有明显的抑制作用,且能抑制JAR细胞MMP-2、MMP-9蛋白的表达。

关键词: 17-AAG, 人绒毛膜癌JAR细胞, 基质金属蛋白酶2（MMP-2）, 基质金属蛋白酶9（MMP-9）

Abstract: Objective: To explore the effects of heat shock protein 90 inhibitor-17-AAG on matrix metalloproteinase-2 (MMP-2) and MMP-9 expression in human choriocarcinoma JAR cells. Methods: The human choriocarcinoma JAR cells were cultured in vitro and treated with 17-AAG in different concentration. MTT was used to measure the proliferation inhibiting rate of JAR cells, Western blotting to detect the MMP-2 and MMP-9 expression. Results: MTT method showed that 17-AAG can obviously inhibit growth of JAR cells, and in concentration dependent manner. After the JAR cells were treated with 17-AAG in different concentration (5μg/ml, 10μg/ml, 20μg/ml, 40μg/ml), the MMP-2 and MMP-9 expression in JAR cells down regulated obviously. Conclusions: 17-AAG can significantly inhibit the proliferation of human choriocarcinoma JAR cells, and can inhibit the expression of MMP-2 and MMP-9 in JAR cells.

Key words: 17-AAG, Human choriocarcinoma JAR cell, MMP-2, MMP-9

中图分类号:

R329.2

谷苗, 崔丽军, 刘百艺, 安国庆, 许倩. 17-AAG对人绒毛膜癌JAR细胞MMP-2和MMP-9表达的调控作用^*[J]. 承德医学院学报, 2018, 35(3): 186-189.

GU Miao, CUI Li-jun, LIU Bai-yi, et al. REGULATORY EFFECTS OF 17-AAG ON MMP-2 AND MMP-9 EXPRESSION IN HUMAN CHORIOCARCINOMA JAR CELLS[J]. Journal of Chengde Medical University, 2018, 35(3): 186-189.

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