香菇多糖抗小鼠MRSA皮肤感染的作用

承德医学院学报 ›› 2021, Vol. 38 ›› Issue (4): 271-283.

• 基础医学 • 下一篇

香菇多糖抗小鼠MRSA皮肤感染的作用

蒋苗苗¹, 李雪飞¹, 唐冠杰¹, 轩玉宏², 段昕所¹, 孙立新¹

1.承德医学院附属医院,河北承德 067000;
2.承德市中心医院

收稿日期:2020-07-27 出版日期:2021-08-10 发布日期:2021-11-18

Effects of Lentinan on MRSA Skin Infection in Mice

JIANG Miao-miao¹, LI Xue-fei¹, TANG Guan-jie¹, XUAN Yu-hong², DUAN Xin-suo¹, SUN Li-xin¹

1. Affiliated Hospital of Chengde Medical University, Chengde, Hebei, 067000, China;
2. Chengde Central Hospital

Received:2020-07-27 Online:2021-08-10 Published:2021-11-18

摘要/Abstract

摘要： 目的本研究主要探讨香菇多糖是否具有抗小鼠耐甲氧西林金黄色葡萄球菌皮肤感染的作用。方法将小鼠随机分为对照组和香菇多糖处理组,每组10只,皮下注射5×10⁷CFU/30μl耐甲氧西林金黄色葡萄球菌,建立小鼠皮肤感染模型。对照组腹腔注射100μl PBS,实验组腹腔注射100μl香菇多糖（100mg/kg）。观察小鼠皮肤部位感染情况,感染组织匀浆细菌培养检测小鼠皮肤感染部位细菌负荷量,免疫荧光染色方法检测小鼠皮肤感染部位内中性粒细胞浸润情况,RT-PCR技术检测小鼠皮肤感染部位白细胞介素33(IL-33)、中性粒细胞趋化因子1(KC)的表达。结果经香菇多糖处理后,小鼠皮肤部位感染明显轻于对照组;感染部位细菌定量、免疫荧光染色显示香菇多糖可降低小鼠皮肤感染部位细菌负荷量,促进中性粒细胞浸润;RT-PCR技术检测显示香菇多糖可促进小鼠皮肤感染部位IL-33、趋化因子KC的表达。结论香菇多糖可抗耐甲氧西林金黄色葡萄球菌对小鼠皮肤的感染。

关键词: 香菇多糖, 抗感染, 耐甲氧西林金黄色葡萄球菌, 中性粒细胞

Abstract: Objective This study was conducted to determine the potential of lentinan in protection against methicillin-resistant S. aureus (MRSA) skin infection. Methods Bacterial burden determination and Immunohistochemistry analysis were used to assess the effect of lentinan on antimicrobial infection using a Staphylococcus aureus-infected skin injection model in mice by inoculated with 30μl of 5×10⁷ CFU MRSA, and the polymerase chain reaction (RT-PCR) was used to test the effects of lentinan on mRNA expression of IL-33 and chemokine KC at skin abscess in mice. Results Skin wound sections from lentinan -treated in mice was significantly milder than that in control group. Bacterial burden determination and immunohistochemistry analysis showed that significantly decrease bacterial burden and significant increase in CD11b+Gr1+ neutrophils recruitment infiltration, was noted in infectious wounds from lentinan -treated mice. At the same time, mRNA expression of IL-33 and chemokine KC was promptly increased in cutaneous wound tissues when challenged by lentinan. Conclusion Lentinan plays an essential effect of anti-MRSA skin infection in mice.

Key words: lentinan, anti-infection, methicillin-resistant S. aureus, neutrophil

中图分类号:

R978

蒋苗苗, 李雪飞, 唐冠杰, 轩玉宏, 段昕所, 孙立新. 香菇多糖抗小鼠MRSA皮肤感染的作用[J]. 承德医学院学报, 2021, 38(4): 271-283.

JIANG Miao-miao, LI Xue-fei, TANG Guan-jie, XUAN Yu-hong, DUAN Xin-suo, SUN Li-xin. Effects of Lentinan on MRSA Skin Infection in Mice[J]. Journal of Chengde Medical University, 2021, 38(4): 271-283.

参考文献

[1] Rincão VP, Yamamoto KA, Ricardo NM, et al.Polysaccharide and extracts from Lentinula edodes: structural features and antiviral activity[J]. Virol. J, 2012, 9: 37.
[2] 胡付品,郭燕,朱德妹,等. 2017年CHINET 中国细菌耐药性监测[J]. 中国感染与化疗杂志,2018,18(3):241-251.
[3] Surya V, Kumar P, Gupta S, et al.Childhood pemphigus vulgaris: report of two cases with emphasis on diagnostic approach[J]. Contemp Clin Dent, 2018, 9(Suppl 2): S373-S376.
[4] 朱心雨,胡雪飞. 耐甲氧西林金黄色葡萄球菌相关研究进展[J]. 实用临床医学,2018,19(11):95-99.
[5] Verdrengh M, Tarkowski A.Role of neutrophils in experimental septicemia and septic arthritis induced by Staphylococcus aureus[J]. Infect Immun 1997, 65(7): 2517-2521.
[6] Rigby KM, Deleo FR.Neutrophils in innate host defense against Staphylococcus aureus infections[J]. Semin Immunopathol, 2012, 34(2): 237-259.
[7] Wang J, Hossain M, Thanabalasuriar A, et al.Visualizing the function and fate of neutrophils in sterile injury and repair[J]. Science, 2017, 358(6359): 111-116.
[8] Florent A, Mohamed B, François M, et al.IL-33/ST2 pathway regulates neutrophil migration and predicts outcome in patients with severe alcoholic hepatitis[J]. Journal of hepatology, 2020, 72(6): 1052-1061.
[9] Chia-Lin H, Krishan D, Rebecca K, et al.Allergic inflammation is initiated by IL-33-dependent crosstalk between mast cells and basophils[J]. PloS one, 2010, 15(1): e0226701. DOI:10.1371/journal.pone.0226701.
[10] VetvickaV. Glucan-immunostimulant, adjuvant , potential drug[J].World J Clin Oncol, 2011, 2(2): 115-119.
[11] Chihara G.Recent progress in immunopharmacology and therapeutic effects of polysaccharides[J]. Dev Biol Stand, 1992, 77: 191-197.
[12] Xu H, Zou S, Xu X, et al.Anti-tumor effect of β-glucan from Lentinus edodes and the underlying mechanism[J]. Sci Rep, 2016, 6: 28802. DOI:10.1038/srep28802.
[13] 李宜真. 香菇多糖对化学治疗后骨髓抑制合并脓毒血症的防护作用及机制研究[J]. 药物研究,2016,5(16):40-42.
[14] 章静,杨成伟,王云,等. 香菇多糖对脓毒症小鼠急性肺损伤的保护作用[J]. 中华医院感染学杂志,2018,28(10):1463-1468.
[15] 戚爱华,孙艳霞,李威,等. 香菇多糖的药用价值[J]. 食品安全导刊,2018,(12):128.

香菇多糖抗小鼠MRSA皮肤感染的作用

Effects of Lentinan on MRSA Skin Infection in Mice

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价