天疱疮患者血清中Dsg1及Dsg3抗体水平检测及意义

承德医学院学报 ›› 2021, Vol. 38 ›› Issue (6): 482-485.

天疱疮患者血清中Dsg1及Dsg3抗体水平检测及意义

刘笑言¹, 王艳心², 余海², 张佳丽¹, 陈丽莉¹, 李保强^1,*

1.承德医学院附属医院皮肤科,河北承德 067000;
2.沧州中西医结合医院皮肤科

收稿日期:2021-09-01 出版日期:2021-12-10 发布日期:2021-12-13
通讯作者: ^*

The Significance of Dsg1 and Dsg3 Antibody's Detection in Pemphigus Patients

LIU Xiao-yan¹, WANG Yan-xin², YU Hai¹, ZHANG Jia-li¹, CHEN Li-li¹, LI Bao-qiang^1,*

Department of dermatology, Affiliated Hospital of Chengde Medical University, Chengde, Heibei, 067000, China;
2. Cangzhou Hospital of Integrated Traditional Chinese and Western, Cangzhou, Hebei, 061000, China

Received:2021-09-01 Online:2021-12-10 Published:2021-12-13

摘要/Abstract

摘要： 目的研究18例天疱疮患者血清中抗桥粒芯糖蛋白抗体1（Dsg1）及抗桥粒芯糖蛋白抗体3（Dsg3）的表达水平和治疗前后的变化以及抗体指数与病情严重程度的相关关系,以探讨Dsg1及Dsg3在天疱疮患者的诊断、监测病情变化及治疗中的应用。方法对18例患者通过病理及直接免疫荧光进行诊断,并用ABSIS评分标准对患者的病情进行评估,应用酶联免疫吸附试验（ELISA）法检测患者及20例正常人血清中Dsg1及Dsg3抗体的水平,最后通过统计分析判断各指标间的关系及意义。结果 18例患者中Dsg1阳性13例,Dsg3阳性10例;治疗后的Dsg抗体指数较治疗前下降,治疗前后的Dsg1差值为3.13±13.52,Dsg3差值为3.81±10.05,差异有统计学意义（P<0.05）;20例正常人血清中,Dsg1阳性2例,阴性18例;Dsg3阳性1例,阴性19例。Dsg1及Dsg3抗体检测的灵敏性分别为72.22%及55.56%,特异性分别为90%及95%。治疗前后的Dsg抗体指数与病情评分分别具有相关性（均为P<0.05）。结论天疱疮患者血清中Dsg1及Dsg3中抗体检测具有较高的灵敏性和特异性,其抗体水平与疾病的严重程度具有一定的相关性,可作为天疱疮的常规辅助检测,并用于监测病情变化及指导治疗。

关键词: 天疱疮, 桥粒芯糖蛋白1, 桥粒芯糖蛋白3, ABSIS评分

Abstract: Objective In order to investigate the application of anti-Dsg1and anti-Dsg3 antibody in diagnosis and treatment of patients, the level and changes of anti-Dsg1and anti-Dsg3 antibody in 18 patients with pemphigus and the relationship between the antibody index and the severity of the disease were analyzed. Methods 18 patients were diagnosed for pemphigus by direct immunofluorescence and pathology, and the patient's pathogenetic condition was assessed by ABSIS standard.The level of Dsg1 and Dsg3 of patients and nomals were detected by enzyme-linked immunosorbent assay(ELISA). Statistics was performed to analyze the relationship and significance of each index at last. Results Among the 18 patients, 13 were Dsg1 positive and 10 Dsg3 positive . The index of Dsg1 and Dsg3 after treatment were decreased than before, and the difference was statistically significant (P<0.05). Of the 20 nomals, 2 were Dsg1 positive and 1 was Dsg3 positive. The sensitivity of Dsg1 and Dsg3 antibody detection was 72.22% and 55.56% respectively, and the specificity was 90% and 95% respectively. The index of Dsg antibody was correlated with the ABSIS score (ALL P<0.05). Conclusion The Dsg antibody level of patients with pemphigus was correlated with the severity of the disease with high sensitivity and specificity. The detection of Dsg antibody level can be used for diagnosis and guidance of state and treatment of the disease.

Key words: Pemphigus, Dsg1, Dsg3, ABSIS score

中图分类号:

R758.66

刘笑言, 王艳心, 余海, 张佳丽, 陈丽莉, 李保强. 天疱疮患者血清中Dsg1及Dsg3抗体水平检测及意义[J]. 承德医学院学报, 2021, 38(6): 482-485.

LIU Xiao-yan, WANG Yan-xin, YU Hai, ZHANG Jia-li, CHEN Li-li, LI Bao-qiang. The Significance of Dsg1 and Dsg3 Antibody's Detection in Pemphigus Patients[J]. Journal of Chengde Medical University, 2021, 38(6): 482-485.

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