膜联蛋白A7低表达对人胃癌MGC-803细胞增殖和凋亡的影响

摘要/Abstract

摘要： 目的探讨膜联蛋白A7（ANXA7）低表达对人胃癌MGC-803细胞增殖和凋亡的影响。方法将人胃癌MGC-803细胞分为ANXA7低表达组（si-ANXA7）和阴性对照组（si-con）,分别将靶向ANXA7的si-RNA和阴性对照RNA转染为ANXA7低表达组和阴性对照组。Western blot法检测ANXA7的表达;MTT法检测各组细胞的增殖能力;PI检测各组细胞的凋亡率;Western blot检测各组细胞增殖和凋亡相关蛋白PCNA和Cleaved casepase-3的表达。结果与阴性对照组相比,ANXA7低表达组MGC-803细胞增殖能力显著降低,增殖相关蛋白PCNA表达显著下降(P<0.05);细胞凋亡增强,相关蛋白Cleaved casepase-3的表达显著升高(P<0.05)。结论 ANXA7低表达可抑制人胃癌MGC-803细胞的增殖并促进其凋亡。

关键词: ANXA7, MGC-803细胞, 增殖, 凋亡

Abstract: Objective To investigate the effects of low-expression of annexin A7 (ANXA7) on the proliferation and apoptosis of human gastric cancer MGC-803 cells. Methods Human gastric cancer MGC-803 cells were divided into ANXA7 low-expression group (si-ANXA7), negative control group (si-con) . Si-RNA targeting ANXA7 and negative control RNA were transfected into ANXA7 low-expression group and negative control group respectively. The expression of ANXA7 was detected by Western blot; MTT assay was used to detect the proliferation of cells in each group; The apoptosis rate in each group was detected by PI; The expressions of PCNA and Cleaved casepase-3 were detected in each group by Western blot. Results Compared with the negative control group, the proliferation ability of MGC-803 cells in the low-expression group of ANXA7 decreased significantly, and the expression of proliferation related proteins PCNA decreased significantly (P<0.05); The expression of cleaved casepase-3 increased significantly (P<0.05). Conclusion The low-expression of ANXA7 may inhibitthe proliferation and promote the apoptosis of human gastric cancer MGC-803 cells.

Key words: ANXA7, MGC-803 cell, proliferation, apoptosis

中图分类号:

R735.2

刘明伟, 柏友兰, 王小杰, 李欣. 膜联蛋白A7低表达对人胃癌MGC-803细胞增殖和凋亡的影响[J]. 承德医学院学报, 2022, 39(1): 5-8.

LIU Ming-wei, BAI You-lan, WANG Xiao-jie, LI Xin. Effects of Low Expression of ANXA7 on Proliferation and Apoptosis of Human Gastric Cancer MGC-803 Cells[J]. Journal of Chengde Medical University, 2022, 39(1): 5-8.

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