梓醇对STZ诱导的糖尿病大鼠肾脏的保护作用

承德医学院学报 ›› 2022, Vol. 39 ›› Issue (3): 192-196.

梓醇对STZ诱导的糖尿病大鼠肾脏的保护作用

张翠¹, 王继胜², 王林¹, 胡泽波¹, 张根葆^1,*

1.皖南医学院病理生理学教研室,安徽芜湖 241002;
2.皖南医学院

收稿日期:2021-01-06 出版日期:2022-06-10 发布日期:2022-08-02
通讯作者: *
基金资助:
安徽省高校优秀青年人才支持计划项目（gxyq2018044）; 皖南医学院重点科研基金项目（WK2021Z09）; 皖南医学院中青年科研基金项目（WK201907）

Protective Effects of Catalpol on the Diabetic Nephropathy in STZ-induced Diabetic Rats

ZHANG Cui¹, WANG Ji-sheng², WANG Lin¹, HU Ze-bo¹, ZHANG Gen-bao^1,*

1. Department of Pathophysiology,Wannan Medical College, Wuhu, Anhui, 241002, China;
2. Department of Anatomy, Wannan Medical College, Wuhu, Anhui, 241002, China

Received:2021-01-06 Online:2022-06-10 Published:2022-08-02

摘要/Abstract

摘要： 目的研究梓醇对糖尿病大鼠肾脏的保护作用及其对S100A8、TLR4、NF-κB表达的影响。方法健康雄性SD大鼠,分为正常对照组、糖尿病模型组,梓醇干预组。采用腹腔注射链脲佐菌素法复制糖尿病模型,检测各组空腹血糖、血肌酐、尿蛋白水平,免疫组织化学方法检测肾组织S100A8、TLR4、NF-κB蛋白的表达情况,HE染色显微镜下观察肾脏形态学改变。结果梓醇干预组大鼠空腹血糖、血肌酐、尿蛋白水平较模型组显著下降,肾组织S100A8、TLR4、NF-κB蛋白的表达量与模型组相比亦显著降低。结论梓醇对链脲佐菌素诱导的糖尿病大鼠具有保护作用,其机制可能与降低大鼠空腹血糖水平及下调肾组织中S100A8、TLR4、NF-κB蛋白表达有关。

关键词: 糖尿病肾病, S100A8, TLR4, NF-κB, 梓醇

Abstract: Objective To investigate the protective effect of catalpol on kidney and the expression of S100A8, TLR4 and NF-κB in diabetic rats. Methods Healthy male sprague-dawley rats were randomly divided into 3 groups: the normal control group, the diabetes model group, and the catalpol intervention group. The diabetic rats were induced by intraperitoneal injection with streptozotocin. The levels of fasting glucose, serum creatinine and urinary protein in each group were detected. The expression of S100A8, TLR4 and NF-κB in kidney were assessed by immunohistochemistry. The morphological changes of renal tissue were checked through microscopy. Results Compared with the model group, the levels of fasting blood glucose, serum creatinine and urinary protein in catalpol group were significantly decreased, and the expression levels of S100A8, TLR4 and NF-κB in renal cortex were dramatically decreased. Conclusion Catalpol has a protective effect on STZ induced diabetic rats, and its mechanism may be related to the decrease of fasting blood glucose level and the down regulation of S100A8, TLR4 and NF-κB protein expression in renal tissue.

Key words: diabetic nephropathy, S100A8, TLR4, NF-κB, catalpol

中图分类号:

R587.2

张翠, 王继胜, 王林, 胡泽波, 张根葆. 梓醇对STZ诱导的糖尿病大鼠肾脏的保护作用[J]. 承德医学院学报, 2022, 39(3): 192-196.

ZHANG Cui, WANG Ji-sheng, WANG Lin, HU Ze-bo, ZHANG Gen-bao. Protective Effects of Catalpol on the Diabetic Nephropathy in STZ-induced Diabetic Rats[J]. Journal of Chengde Medical University, 2022, 39(3): 192-196.

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