Lnc RNA CUDR敲低对肝癌侵袭、增殖和凋亡的影响

承德医学院学报 ›› 2022, Vol. 39 ›› Issue (3): 196-200.

Lnc RNA CUDR敲低对肝癌侵袭、增殖和凋亡的影响

王波¹, 金雯^1,*, 周健坤¹, 陈良², 曹立宇³

1.铜陵职业技术学院医学护理系,安徽铜陵 244061;
2.合肥市第二人民医院高压氧科;
3.安徽医科大学第一附属医院病理科

收稿日期:2021-06-21 出版日期:2022-06-10 发布日期:2022-08-02
通讯作者: *
基金资助:
安徽高校自然科学研究重点项目（KJ2020A0974）; 安徽高校优秀青年人才支持计划重点项目（gxydZD2021155）; 安徽省高等学校省级质量工程项目（2019cxtd043）

Effects of Lnc RNA CUDR Knock-down on Invasion, Proliferation and Apoptosis of Hepatocellular Carcinoma

WANG Bo¹, JIN Wen^1,*, ZHOU Jian-kun¹, CHEN Liang², CAO Li-yu³

1. Department of Madical and Nursing, Tongling Polytechnic College, Tongling, Anhui, 244061, China;
2. Department of Hyperbaric oxygen, The second people's Hospital of Hefei, Hefei, Anhui, 230011, China;
3. Department of Pathology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China

Received:2021-06-21 Online:2022-06-10 Published:2022-08-02

摘要/Abstract

摘要： 目的探讨LncRNA CUDR敲低对肝癌细胞侵袭、增殖和凋亡的影响。方法采用CUDR shRNA慢病毒质粒转染SMMC-7721肝癌细胞系,分别设CUDR敲低组、对照组和空质粒组,采用RT-PCR方法检测CUDR shRNA转染效率;Transwell检测3组细胞的侵袭能力;瘤球成形实验检测细胞聚集、增殖能力;流式技术检测肝癌细胞凋亡率。结果 RT-PCR检测结果显示,CUDR shRNA转染SMMC-7721肝癌细胞系后,与对照组、空质粒组相比,敲低组CUDR mRNA表达量显著下降（P<0.001）;Transwell、瘤球成形实验和细胞凋亡实验结果显示,与对照组和空质粒组相比,CUDR敲低组肝癌细胞的侵袭与聚集能力显著下降且凋亡率提高（P<0.001）。结论敲低LncRNA CUDR表达可抑制HCC的侵袭和增殖,并促进肝癌细胞的凋亡,LncRNA CUDR具有成为治疗HCC潜在靶点的价值。

关键词: 肝细胞癌, 长链非编码RNA CUDR, 侵袭, 增殖, 凋亡

Abstract: Objective To investigate the effect of LncRNA CUDR knock-down on invasion, proliferation and apoptosis of hepatocellular carcinoma cells. Methods CUDR shRNA lentivirus plasmid was used to transfect SMMC-7721 hepatoma cell line. The CUDR knockdown group, control group and empty plasmid group were set up respectively. The transfection efficiency of CUDR shRNA was detected by RT-PCR method, the invasive ability of the three groups was detected by Transwell, the ability of cell aggregation and proliferation was detected by tumor ball formation test, and the apoptosis rate of hepatocellular carcinoma cells was detected by flow cytometry. Results The results of RT-PCR detection showed that the expression of CUDR mRNA in the knockdown group was significantly lower than that in the control group and empty plasmid group after CUDR shRNA transfection（P<0.001）. The results of Transwell, tumor ball formation test and apoptosis assay showed that the invasion and aggregation abilities of hepatoma cells in the CUDR knockdown group were significantly lower than those in the control group and the empty plasmid group, and the apoptosis rate was increased in CUDR knockdown group(P<0.001). Conclusion Knocking down the expression of LncRNA CUDR can inhibit the invasion and proliferation of HCC and promote the apoptosis of hepatocellular carcinoma cells, LncRNA CUDR may be a potential therapeutic target for HCC.

Key words: hepatocellular carcinoma, long chain non-coding RNA CUDR, invasion, proliferation, apoptosis

中图分类号:

R735.7

王波, 金雯, 周健坤, 陈良, 曹立宇. Lnc RNA CUDR敲低对肝癌侵袭、增殖和凋亡的影响[J]. 承德医学院学报, 2022, 39(3): 196-200.

WANG Bo, JIN Wen, ZHOU Jian-kun, CHEN Liang, CAO Li-yu. Effects of Lnc RNA CUDR Knock-down on Invasion, Proliferation and Apoptosis of Hepatocellular Carcinoma[J]. Journal of Chengde Medical University, 2022, 39(3): 196-200.

参考文献

[1] Sung H, Ferlay J, Siegel RL, et al.Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71(3): 209-249.
[2] Guo X, Wang Y.LncRNA TMPO-AS1 promotes hepatocellular carcinoma cell proliferation, migration and invasion through sponging miR-329-3p to stimulate FOXK1-mediated AKT/mTOR signaling pathway[J]. Cancer Med, 2020, 9(14): 5235-5246.
[3] Yang GH, Zhou LJ, Xu QH, et al.LncRNA KCNQ1OT1 inhibits the radiosensitivity and promotes the tumorigenesis of hepatocellular carcinoma via the miR-146a-5p/ACER3 axis.[J]. Cell cycle, 2020, 19(19): 2519-2529.
[4] Liang X, Qi My, Wu R, et al.Long non-coding RNA CUDR promotes malignant phenotypes in pancreatic ductal adenocarcinoma via activating AKT and ERK signaling pathways[J]. International Journal of Oncology, 2018, 53(6): 2671-2682.
[5] Zhou J, Sun H, Wang Z, et al.Guidelines for the diagnosis and treatment of hepatocellular carcinoma(2019 edition)[J]. Liver Cancer, 2020, 9(6): 682-720.
[6] Neophytou CM, Kyriakou TC, Papageorgis P.Mechanisms of metastatic tumor dormancy and implications for cancer therapy[J]. Int J Mol Sci, 2019, 20(24): 6158.
[7] 马燕,戴科军,恽一飞,等. 微小RNA-1258靶向调控Wnt7b对肝癌细胞迁移侵袭和Wnt/β-catenin通路的影响[J]. 临床肿瘤学杂志,2021,26(4):329-334.
[8] Hou ZH, Xu XW, Fu XY, et al.Long non-coding RNA MALAT1 promotes angiogenesis and immunosuppressive properties of HCC cells by sponging miR-140[J]. Am J Physiol Cell Physiol, 2020, 318(3): 649-663.
[9] Hu W, Feng H, Xu XY, et al.Long noncoding RNA FOXD2‐AS1 aggravates hepatocellular carcinoma tumorigenesis by regulating the miR‐206/MAP3K1 axis[J]. Cancer Medicine, 2020, 9(15): 5620-5631.
[10] 吴惧,李贺,岳坤,等. 长链非编码RNA MALAT1通过PI3K/Akt信号传导通路调控肝细胞癌生物学行为的研究[J]. 中国实验诊断学,2021,25(4):564-569.
[11] Yu YB, Li M, Xu JB, et al.Over expression of long noncoding RNA CUDR promotes hepatic differentiationof human umbilical cord mesenchymal stem cells[J]. Molecular Medicine Reports, 2020, 21(3): 1051-1058.
[12] Wu MY, An JH, Zheng QD, et al.Double mutant P53 (N340Q/L344R) promotes hepatocarcinogenesis through upregulation of Pim1 mediated by PKM2 and LncRNA CUDR[J]. Oncotarget, 2016, 7(41): 66525-66539.
[13] Zheng QD, Lin Z, Li X, et al.Inflammatory cytokine IL6 cooperates with CUDR to aggravate hepatocyte-like stem cells malignant transformation through NF-κB signaling[J]. Scientific Reports, 2016, 6(3): 143-151.

[1]	戈念念, 闫向敏, 闫如冰, 刘建军. 血清HIF-1α、sFas、Livin在老年VaD中的表达及临床意义[J]. 承德医学院学报, 2022, 39(3): 200-204.
[2]	柳雪, 刘慧, 霍峰, 徐树雷, 陶亚东. 干扰素调节因子1对舌鳞癌细胞增殖侵袭和迁移的影响[J]. 承德医学院学报, 2022, 39(2): 102-106.
[3]	刘明伟, 柏友兰, 王小杰, 李欣. 膜联蛋白A7低表达对人胃癌MGC-803细胞增殖和凋亡的影响[J]. 承德医学院学报, 2022, 39(1): 5-8.
[4]	吴现磊, 秦二云, 孟妍, 张令巧. miR-638靶向下调MACC1抑制胃癌细胞的恶性进展[J]. 承德医学院学报, 2021, 38(6): 451-457.
[5]	柏友兰, 刘明伟, 王小杰, 李欣. 膜联蛋白A7低表达对人胃癌MGC-803细胞迁移和侵袭的影响[J]. 承德医学院学报, 2021, 38(5): 374-378.
[6]	王小杰, 柏友兰, 刘明伟, 王新杰, 李宏伟, 李欣. 膜联蛋白A7过表达对人胃癌MGC-803细胞增殖和凋亡的影响[J]. 承德医学院学报, 2021, 38(4): 288-291.
[7]	祝盼盼, 商亚珍. MAPK信号通路介导细胞凋亡的研究进展[J]. 承德医学院学报, 2021, 38(3): 243-245.
[8]	李美川, 刘明伟, 赵依纳, 王小杰, 李欣. LncRNA TCONS_00022381在胃癌组织中的表达及其对胃癌SGC-7901细胞增殖的影响[J]. 承德医学院学报, 2021, 38(2): 96-99.
[9]	方佩斐, 陈谨, 宋先兵, 方永红. Kiss-1基因过表达对直肠癌SW620细胞增殖的影响[J]. 承德医学院学报, 2020, 37(6): 456-459.
[10]	杨爱峰, 侯鹏飞. miR-145-5p靶向MEST抑制胃癌上皮细胞增殖侵袭转移的研究[J]. 承德医学院学报, 2020, 37(6): 451-455.
[11]	李佳欣, 齐洁敏. 炎症因子IL-32、凋亡因子Apaf-1和胃癌[J]. 承德医学院学报, 2020, 37(3): 247-250.
[12]	刘赛璇, 薛晶, 周丽丽, 赵杨, 申兴斌. 鬼臼苦素对人乳腺癌MCF-7细胞增殖的抑制作用[J]. 承德医学院学报, 2020, 37(1): 5-7.
[13]	王虹. OSU-03012对人结肠癌LoVo细胞增殖侵袭及COX-2、VEGF、MMP-2表达的影响[J]. 承德医学院学报, 2019, 36(6): 451-455.
[14]	张怡, 高维娟. Bcl-2/Beclin1复合物对细胞凋亡与自噬的调节作用^*[J]. 承德医学院学报, 2019, 36(4): 335-340.
[15]	李颖, 马洪伟, 付秀美, 陈志宏, 薛景凤. 远志对DPN大鼠背根节神经元损伤的预防保护作用^*[J]. 承德医学院学报, 2019, 36(4): 274-277.