miR-493-5p、CHE联合GP73检测在肝癌筛查、病情评估中的应用研究

摘要/Abstract

摘要： 目的探讨miR-493-5p、胆碱脂酶（CHE）联合高尔基体糖蛋白73（GP73）检测在肝癌筛查、病情评估中的应用价值。方法选取2015年7月~2019年7月安阳市第五人民医院收治的98例肝癌患者（临床分期：早期31例、中期35例、晚期32例）作为观察组,选取同期本院的健康体检者104例作为对照组。聚合酶链式反应-限制性片段长度多态性法检测miR-493-5,丁酰基硫代胆碱法测定CHE水平,电化学发光法检测GP73水平。比较3项指标在不同组别之间的表达水平,分析3者联合检测对肝癌患者病情进展预测的价值。结果观察组miR-493-5p、CHE水平低于对照组,GP73水平高于对照组,差异具有统计学意义（P<0.05）;miR-493-5p相对表达量及CHE水平比较：早期>中期>中晚期,差异具有统计学意义（P<0.05）;GP73水平比较：早期<中期<晚期,差异具有统计学意义（P<0.05）;联合miR-493-5p、CHE、GP73检测灵敏度、特异度、AUC分别为92.10%、94.50%、0.960,均高于单一指标检测,差异具有统计学意义（P<0.05）。结论临床可加强联合miR-493-5p、CHE、GP73检测,用于诊断早期肝癌和评估患者病情进展情况。

关键词: miR-493-5p, CHE, GP73, 肝癌筛查, 病情评估

Abstract: Objective To explore the application value of miR-493-5p, cholinesterase (CHE) combined with Golgiglycoprotein73 (GP73) detection in screening of liver cancer and disease assessment. Methods Ninety-eight patients with liver cancer (clinical stages: 31 cases in early stage, 35 cases in middle stage, and 32 cases in late stage) admitted to Anyang Fifth People's Hospital from July 2015 to July 2019 were selected as the observation group, and 104 patients who had healthy physical examination in this hospital during the same period were selected as the control group. The polymerase chain reaction-restriction fragment length polymorphism method was used to detect miR-493-5, and the butyrylthio-choline method was used to determine the level of CHE, and the electrochemiluminescence method was used to determine the level of GP73. The expression levels of the three indicators in different groups were compared, and the predictive value of the three combined detection on the progression of patients with liver cancer were analyzed. Results The levels of miR-493-5p and CHE in the observation group were lower than those in the control group, and the level of GP73 was higher than that in the control group, the differences were statistically significant (P<0.05). The relative expression of miR-493-5p and the level of CHE were compared: early stage>mid stage>mid-late stage, the differences were statistically significant (P<0.05). The level of GP73 was compared: early stage<mid stage<late stage, the difference was statistically significant (P<0.05). The sensitivity, specificity and AUC of the combined detection of miR-493-5p, CHE, and GP73 were 92.10%, 94.50%, and 0.960, respectively, which were higher than those of single index detection, and the differences were statistically significant (P<0.05). Conclusion In clinic, the combined detection of miR-493-5p, CHE, and GP73 can be strengthened for the diagnosis of early liver cancer and the disease evaluation of patients.

Key words: miR-493-5p, CHE, GP73, screening for liver cancer, disease evaluation

中图分类号:

R735.7

李俊娜, 韩晓亮, 谢东文, 赵根成. miR-493-5p、CHE联合GP73检测在肝癌筛查、病情评估中的应用研究[J]. 承德医学院学报, 2022, 39(3): 205-208.

LI Jun-na, HAN Xiao-liang, XIE Dong-wen, ZHAO Gen-cheng. Application of miR-493-5p, CHE combined with GP73 Detection in Screening of Liver Cancer and Disease Assessment[J]. Journal of Chengde Medical University, 2022, 39(3): 205-208.

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