丝胶对STZ致损伤的INS-1细胞的保护作用

摘要/Abstract

摘要： 目的探讨丝胶对链脲佐菌素（STZ）致损伤大鼠胰岛素瘤细胞（INS-1细胞）的保护作用。方法实验以体外培养的INS-1细胞为研究对象,随机分为5组：正常对照组（常规条件培养细胞）、模型组（培养基中含10mmol/L的STZ）、丝胶低、中、高浓度组（培养基中分别含10mmol/L的STZ,及150μg/mL、300μg/mL、600μg/mL的丝胶）,每组细胞药物作用时间均为24h。蛋白印迹和实时荧光定量PCR法检测各组细胞BCL-2与P53蛋白及mRNA的表达情况。流式细胞术检测各组细胞的凋亡情况。结果与正常对照组相比,在模型组中,INS-1细胞中BCL-2蛋白和mRNA的表达显著减少,P53蛋白和mRNA的表达显著增加,早期凋亡率显著升高（P<0.05）。与模型组相比,丝胶各组INS-1细胞BCL-2蛋白与mRNA的表达均显著增多,P53的蛋白与mRNA的表达均显著下降,早期凋亡率显著下降（P<0.05）;3个丝胶组两两比较差异均有统计学意义（P<0.05）。结论丝胶对STZ致损伤INS-1细胞发挥保护作用的机制与调控凋亡蛋白,抑制INS-1细胞凋亡有关。

关键词: 丝胶, 糖尿病, INS-1细胞, 凋亡

Abstract: Objective To explore the protective effects of sericin on streptozotocin(STZ)-induced injury rats’ INS-1 cells. Methods INS-1 cells were cultured in vitro and randomly divided into five groups. Normal control group (culture under conventional conditions), model group (10mmol/L STZ), low sericin group (STZ+150μg/mL sericin), medium sericin group (STZ+300μg/mL sericin), high sericin group (STZ+600μg/mL sericin). The cells in five groups were cultured respectively with corresponding drugs for 24h. The protein and mRNA expressions of BCL-2 and P53 were detected by Western blotting and real-time PCR. Cell apoptosis was detected by flow cytometry. Results Compared with the normal control group, the expression of BCL-2 protein and mRNA of INS-1 cells in the model group were remarkably decreased, while the expression of P53 protein and mRNA were remarkably increased (P<0.05), as well as the early apoptosis rate was remarkably increased (P<0.05). Compared with the model group, the expression of BCL-2 protein and mRNA in 3 sericin groups were remarkably increased, while the expression of P53 protein and mRNA were remarkably decreased (P<0.05), and the early apoptosis rate was remarkably decreased (P<0.05). Moreover, there were statistically remarkable differences among the 3 sericin groups (P<0.05). Conclusion The protective effects of sericin on STZ-induced injury INS-1 cells is related to the regulation of apoptotic proteins and inhibition of INS-1 cell apoptosis.

Key words: sericin, diabetes, INS-1 cells, apoptosis

中图分类号:

R587.1

李金尧, 韩思雨, 李雨欣, 易梦雅, 陈志宏. 丝胶对STZ致损伤的INS-1细胞的保护作用[J]. 承德医学院学报, 2023, 40(2): 91-95.

LI Jin-yao, HAN Si-yu, LI Yu-xin, YI Meng-ya, CHEN Zhi-hong. Protective Effects of Sericin on STZ-induced Injury INS-1 Cells[J]. Journal of Chengde Medical University, 2023, 40(2): 91-95.

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