血清可溶性TLR2和TLR4与支气管肺炎和大叶性肺炎住院儿童的相关性研究

承德医学院学报 ›› 2023, Vol. 40 ›› Issue (4): 280-285.

血清可溶性TLR2和TLR4与支气管肺炎和大叶性肺炎住院儿童的相关性研究

程婷婷¹, 麻雯熠², 肖梦¹, 李涛¹, 谢广成^2,*, 刘建英^1,*

1.承德医学院第二临床教学医院,河北承德 067000;
2.承德医学院基础医学院

收稿日期:2022-09-29 出版日期:2023-08-10 发布日期:2023-09-15
通讯作者: *
基金资助:
蚌埠医学院自然科学重点专项（2020BYZD110）; 安徽高校自然科学研究重点项目（KJ2021A0820）

Association between Serum Soluble TLR2 and TLR4 with Bronchopneumonia and Loba Pneumonia in Hospitalized Children

CHENG Ting-ting¹, MA Wen-yi², XIAO Meng¹, LI Tao¹, XIE Guang-cheng^2,*, LIU Jian-ying^1,*

1. No.2 Clinical Teaching Hospital of Chengde Medical University, Chengde, Hebei, 067000, China;
2. School of Basic Medicine,Chengde Medical University

Received:2022-09-29 Online:2023-08-10 Published:2023-09-15

摘要/Abstract

摘要： 目的探讨支气管肺炎和大叶性肺炎患儿血清可溶性TLR2和TLR4的变化特征。方法选择2021年11月~2022年1月承德医学院第二临床教学医院儿科收治的支气管肺炎和大叶性肺炎患儿50例,同时选择同期医院进行体检的健康儿童9名作为对照组。酶联免疫吸附试验检测收集的支气管肺炎、大叶性肺炎患儿和健康儿童血清可溶性TLR2和TLR4水平。结果 M. pneumoniae感染所致大叶性肺炎患儿血清sTLR4水平显著高于对照组（P<0.05）,sTLR2水平呈现明显动力学变化特征（P<0.05）;M. pneumoniae感染所致支气管肺炎患儿血清sTLR2水平和sTLR4水平显著高于对照组（P<0.05）;与M. pneumoniae感染所致大叶性肺炎患儿相比,M. pneumoniae感染所致支气管肺炎患儿血清具有较高水平的sTLR2和较低水平的sTLR4（P<0.05）;与单纯非M. pneumoniae感染所致支气管肺炎患儿相比,混合非M. pneumoniae感染所致支气管肺炎患儿血清具有较高水平的sTLR2和sTLR4（P<0.05）。结论支气管肺炎和大叶性肺炎患儿血清sTLR2和sTLR4呈现高表达水平,临床诊治可以参考血清sTLR2和sTLR4水平对肺炎类型和病情进行评估。

关键词: 肺炎支原体, 可溶性TLR2, 可溶性TLR4, 支气管肺炎, 大叶性肺炎

Abstract: Objective To investigate the changes of soluble TLR2 (sTLR2) and TLR4 (sTLR4) in serum of hospitalized children with bronchopneumonia and lobar pneumonia. Methods A total of 50 hospitalized children with bronchopneumonia and lobar pneumonia was collected at Department of Pediatrics of NO.2 Clinical Teaching Hospital of Chengde Medical University from November 2021 to January 2022. Meanwhile, 9 healthy children who received physical examination were also collected and set as the control group at the same period. The levels of sTLR2 and sTLR4 in serum of hospitalized children with bronchopneumonia and lobar pneumonia and healthy children were determined using enzyme-linked immune sorbent assay (ELISA) kit. Result sLevel of sTLR4 in hospitalized children with lobar pneumonia which caused by Mycoplasma pneumoniae (M. pneumoniae) was higher than those of control group (P<0.05) and level of sTLR2 appeared significantly dynamic characteristics (P<0.05). Levels of sTLR2 and sTLR4 in hospitalized children with bronchopneumonia which caused by M. pneumoniae were significantly higher than those of control group (P<0.05). Compared with hospitalized children with lobar pneumonia which caused by M. pneumoniae, level of sTLR2 was significantly higher and level of sTLR4 was significantly lower in hospitalized children with bronchopneumonia which caused by M. pneumoniae (P<0.05). Compared with hospitalized children with sole bronchopneumonia which caused by non-M. pneumoniae, levels of sTLR2 and sTLR4 were significantly higher in hospitalized children with combined bronchopneumonia which caused by non-M. pneumoniae (P<0.05). Conclusion Levels of sTLR2 and sTLR4 in serum of children with bronchopneumonia and lobar pneumonia appear higher expression manner. The levels of sTLR2 and sTLR4 in serum have certain value in judging types of pneumonia and assessing illness condition for clinicians.

Key words: Mycoplasma pneumoniae, soluble TLR2, soluble TLR4, bronchopneumonia, lobar pneumonia

中图分类号:

R725.1

程婷婷, 麻雯熠, 肖梦, 李涛, 谢广成, 刘建英. 血清可溶性TLR2和TLR4与支气管肺炎和大叶性肺炎住院儿童的相关性研究[J]. 承德医学院学报, 2023, 40(4): 280-285.

CHENG Ting-ting, MA Wen-yi, XIAO Meng, LI Tao, XIE Guang-cheng, LIU Jian-ying. Association between Serum Soluble TLR2 and TLR4 with Bronchopneumonia and Loba Pneumonia in Hospitalized Children[J]. Journal of Chengde Medical University, 2023, 40(4): 280-285.

参考文献

[1] Liu L, Oza S, Hogan D, et al.Global, regional, and national causes of child mortality in 2000-13, with projections to inform post-2015 priorities: an updated systematic analysis[J]. Lancet, 2015, 385(9966): 430-440.
[2] Su M, Wang Q, Li D, et al.Prevalence and clinical characteristics of hospitalized children with community-acquired Mycoplasma pneumoniae pneumonia during 2017/2018, Chengde, China[J]. Medicine (Baltimore), 2021, 100(5): e23786.
[3] Li D, Wang Q, Su M, et al.Molecular Epidemiology and Clinical Features of Haemophilus influenzae among Hospitalized Children with Community-acquired Pneumonia in Chengde, China[J]. Biomed Environ Sci, 2020, 33(8): 623-627.
[4] 王倩,苏萌,李丹,等. 333例社区获得性肺炎住院儿童中人偏肺病毒和博卡病毒的分子流行及临床特征[J]. 中华实验和临床病毒学杂志,2019(3):261-266.
[5] 刘洪杉,金欣,张楠,等. sTLR2和sTLR4在感染性疾病和自身免疫性疾病中的研究进展[J]. 国际免疫学杂志,2020,43(5):547-552.
[6] 陈金妮,李大伟,林秋玉,等. 难治性肺炎患儿感染病原学及HMGB1、TLR2、TLR4表达[J]. 中华医院感染学杂志,2020,30(18):2847-2850.
[7] 冯冰瑜,叶洪舟,凌寅杰,等. 外周血TLRs在儿童难治性支原体肺炎临床诊断和预后评估中的应用[J]. 现代实用医学,2021,33(8):1025-1026.
[8] 王倩,王毅,杜娈英,等. 重症肺炎支原体肺炎患儿的临床资料及影像学特点分析[J]. 医学理论与实践,2019,32(6):894-896.
[9] Yang M, Meng F, Gao M, et al.Cytokine signatures associate with disease severity in children with Mycoplasma pneumoniae pneumonia[J]. Sci Rep, 2019, 9(1): 17853.
[10] Esposito S, Droghetti R, Bosis S, et al.Cytokine secretion in children with acute Mycoplasma pneumoniae infection and wheeze[J]. Pediatr Pulmonol, 2002, 34(2): 122-127.
[11] Xu XF, Li XJ, Liu JL, et al.Serum cytokine profile contributes to discriminating M. pneumoniae pneumonia in children[J]. Cytokine, 2016, 86: 73-78.
[12] Li M, Chen Y, Li H, et al.Serum CXCL10/IP-10 may be a potential biomarker for severe Mycoplasma pneumoniae pneumonia in children[J]. BMC Infect Dis, 2021, 21(1): 909.
[13] 谢广成,段招军. 抗病毒感染固有免疫应答的信号转导[J]. 病毒学报,2012,28(3):303-310.
[14] 于莉,潘靖丹,杜娈英,等. TLR2和TLR4在抗病毒天然免疫应答中的新作用[J]. 病毒学报,2018,34(4):570-578.
[15] Tamiya S, Yoshikawa E, Ogura M, et al.Neutrophil-Mediated Lung Injury Both via TLR2-Dependent Production of IL-1alpha and IL-12 p40, and TLR2-Independent CARDS Toxin after Mycoplasma pneumoniae Infection in Mice[J]. Microbiol Spectr, 2021, 9(3): e158821.
[16] Chen M, Deng H, Zhao Y, et al.Toll-Like Receptor 2 Modulates Pulmonary Inflammation and TNF-alpha Release Mediated by Mycoplasma pneumoniae[J]. Front Cell Infect Microbiol, 2022, 12: 824027.
[17] Luo H, He J, Qin L, et al.Mycoplasma pneumoniae lipids license TLR-4 for activation of NLRP3 inflammasome and autophagy to evoke a proinflammatory response[J]. Clin Exp Immunol, 2021, 203(1): 66-79.
[18] 吴少辉,黄晓冬,徐智航. TLRs信号通路和炎症因子的表达与反复呼吸道感染的关系分析[J]. 中国儿童保健杂志,2021,29(2):205-208.
[19] 张新黎,郭昌云. 重症肺炎患者炎症因子水平和TLR2、TLR4的关系研究[J]. 解放军预防医学杂志,2019,37(2):114-115.
[20] El-Kharashy G, Gowily A, Okda T, et al.Association between serum soluble Toll-like receptor 2 and 4 and the risk of breast cancer[J]. Mol Clin Oncol, 2021, 14(2): 38.
[21] Fakhry N, Gowily A, Okda T, et al.Serum soluble toll-like receptor 2 and 4 as diagnostic and prognostic biomarkers for non-Hodgkin lymphoma[J]. Contemp Oncol (Pozn), 2020, 24(3): 157-162.
[22] Elkammah M, Gowily A, Okda T, et al.Serum soluble Toll-like receptor 4 and the risk of hepatocellular carcinoma in hepatitis C virus patients[J]. Contemp Oncol (Pozn), 2020, 24(4): 216-220.
[23] 李万华. 重症肺炎合并急性肾损伤患者血清TLR4、NF-κB水平变化及其临床意义[J]. 齐齐哈尔医学院学报,2021,42(15):1297-1300.
[24] 陈源浩,杨在东,张小芹,等. 血清和肽素、Toll样受体2、Toll样受体4水平与肺炎支原体感染所致重症肺炎患儿的病情、预后的关系探讨[J]. 药物评价研究,2020,43(11):2275-2279.