ISSN 1004-6879

CN 13-1154/R

 

承德医学院学报 ›› 2025, Vol. 42 ›› Issue (1): 1-6.

• 基础医学 •    下一篇

沉默YTHDF3表达对HCT-116细胞增殖、迁移和凋亡的影响

周涛1, 付溶川1, 唐熙峻1, 孙雨1, 郑帅2, 赵恩宏2, 肖丽君1,*   

  1. 1.承德医学院基础医学院,河北承德 067000;
    2.承德医学院附属医院,河北承德 067000
  • 收稿日期:2024-06-24 出版日期:2025-02-10 发布日期:2025-02-24
  • 通讯作者: *

The Effect of Silencing the Expression of YTHDF3 on the Proliferation, Migration, and Apoptosis of HCT-116 Cells

ZHOU Tao FU Rong-chuan TANG Xi-jun SUN Yu ZHENG Shuai ZHAO En-hong XIAO Li-jun ZHOU Tao1, FU Rong-chuan1, TANG Xi-jun1, SUN Yu1, ZHENG Shuai2, ZHAO En-hong2, XIAO Li-jun1,*   

  1. 1. Basic Medical Institute of Chengde Medical University, Chengde, Hebei, 067000, China;
    2. Affiliated Hospital of Chengde Medical University, Chengde, Hebei, 067000, China
  • Received:2024-06-24 Online:2025-02-10 Published:2025-02-24

摘要: 目的 探究YTH N6甲基腺苷RNA结合蛋白3(YTHDF3)对人结直肠癌细胞系HCT-116增殖、迁移和凋亡的影响。方法 将细胞分为阴性对照组、干扰组1、干扰组2、干扰组3,在结直肠癌细胞HCT-116中转染小干扰YTHDF3,通过qRT-PCR和Western blot实验检测各组细胞YTHDF3表达。采用CCK-8实验、平板克隆实验检测各组细胞增殖能力,Transwell实验和划痕实验检测各组细胞迁移能力,流式细胞术检测各组细胞凋亡能力。结果 通过qRT-PCR和Western blot实验验证HCT-116细胞沉默效率。与阴性对照组相比,在HCT-116细胞中转染小干扰YTHDF3后,干扰组2和干扰组3能有效降低YTHDF3的表达;沉默YTHDF3能显著抑制HCT-116细胞增殖和迁移,促进细胞凋亡。结论 沉默YTHDF3能够减缓HCT-116细胞增殖和迁移,促进HCT-116细胞凋亡。

关键词: 结直肠癌, YTHDF3, HCT-116, 增殖, 迁移, 凋亡

Abstract: Objective To investigate the impact of YTH N6 methyladenosine RNA binding protein 3 (YTHDF3) on the proliferation, migration, and apoptosis of human colorectal cancer cell line HCT-116. Methods The cells were divided into a negative control group, interference groups 1, 2, and 3. Small interfering RNA targeting YTHDF3 was transfected into colorectal cancer cells HCT-116. The expression of YTHDF3 in each group was assessed using qRT-PCR and Western blot analysis. Cell proliferation was evaluated using CCK-8 assay and plate cloning assay, while cell migration was assessed using transwell assay and scratch assay. Flow cytometry was used to measure cell apoptosis. Results The silencing efficiency of HCT-116 cells was validated through qRT-PCR and Western blot analysis. Transfection of small interfering YTHDF3 effectively reduced the expression of YTHDF3 in HCT-116 cells compared to the negative control group. Silencing YTHDF3 can significantly inhibited cell proliferation and migration while promoting apoptosis in HCT-116 cells. Conclusion Silencing YTHDF3 can decelerates cell proliferation and migration while enhancing apoptosis in HCT-116 cells.

Key words: Colorectal cancer, YTHDF3, HCT-116, Proliferation, Migration, Apoptosis

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