SMAD4、SOX2、P53在宫颈癌组织中的变化及其临床意义

摘要/Abstract

摘要： 目的探究Smad同源物4（SMAD4）、性别决定区Y框蛋白2（SOX2）、P53基因（P53）在宫颈癌（CCA）患者癌组织中的表达变化及其对预后的预测价值。方法回顾性选取2021年1月~2022年1月漯河市中心医院收治的CCA患者82例为观察组,另选取良性肿瘤患者82例为对照组,对比2组不同病理特征下SMAD4、SOX2、P53的表达,分析各指标与CCA病理特征的相关性,并探讨其与CCA患者预后的关系。结果观察组SOX2、P53表达情况高于对照组,SMAD4表达情况低于对照组（P<0.05）;Ⅰ~Ⅱ期患者SOX2、P53表达情况低于Ⅲ~Ⅳ期,高分化患者低于中、低分化,SMAD4表达情况高于Ⅲ~Ⅳ期,高分化患者高于中、低分化（P<0.05）;CCA患者SOX2、P53表达情况与TNM分期呈正相关,与分化程度呈负相关,SMAD4表达情况与TNM分期呈负相关,与分化程度呈正相关（P<0.05）;SOX2、P53表达阳性患者2年生存率低于阴性患者,SMAD4表达阳性患者2年生存率高于阴性患者（P<0.05）。结论 CCA组织中SOX2、P53表达异常升高,SMAD4表达异常降低,三项指标与CCA发生、TNM分期、分化程度密切相关,可为临床预后预测提供有效参考。

关键词: 宫颈癌, SMAD4, SOX2, P53, 预后

Abstract: Objective To investigate the expression changes of Smad homolog 4 (SMAD4), sex-determining region Y-box protein 2 (SOX2), and P53 gene (P53) in cancer tissues of patients with cervical cancer (CCA), as well as their predictive value for prognosis. Methods A total of 82 patients with CCA admitted to Luohe Central Hospital from January 2021 to January 2022 were retrospectively selected, serving as the observation group, and another 82 patients with benign tumors were selected as the control group. The expression levels of SMAD4, SOX2, and P53 were compared between the two groups with different pathological characteristics. The correlation between each indicator and the pathological characteristics of CCA was analyzed, and the relationship between these indicators and the prognosis of CCA patients was explored. Results The expression levels of SOX2 and P53 in the observation group were higher than those in the control group, while the expression level of SMAD4 was lower (P<0.05). The expression levels of SOX2 and P53 in patients with stages Ⅰ-Ⅱ were lower than those in patients with stages Ⅲ-Ⅳ, and those in high-differentiated patients were lower than those in moderately and low differentiated patients. The expression level of SMAD4 was higher in patients with stages Ⅲ-Ⅳ than in patients with stages Ⅰ-Ⅱ, and higher in well-differentiated patients than in moderately and poorly differentiated patients (P<0.05). The expression of SOX2 and P53 in CCA patients was positively correlated with TNM staging and negatively correlated with differentiation degree, while the expression of SMAD4 was negatively correlated with TNM staging and positively correlated with differentiation degree (P<0.05). The 2-year survival rate of patients with positive SOX2 and P53 expression was lower than that of patients with negative expression, while the 2-year survival rate of patients with positive SMAD4 expression was higher than that of patients with negative expression (P<0.05). Conclusion In CCA tissues, there is an abnormal increase in the expression of SOX2 and P53, and an abnormal decrease in the expression of SMAD4. These three indicators are closely related to the occurrence, TNM staging, and differentiation degree of CCA, and can provide effective references for clinical prognosis prediction.

Key words: Cervical cancer, SMAD4, SOX2, P53, Prognosis

中图分类号:

R737.3

施稳稳, 王准证. SMAD4、SOX2、P53在宫颈癌组织中的变化及其临床意义[J]. 承德医学院学报, 2025, 42(3): 196-200.

SHI Wen-wen, WANG Zhun-Zheng. Changes in SMAD4, SOX2, and P53 in Cervical Cancer Tissues and Their Clinical Significance[J]. Journal of Chengde Medical University, 2025, 42(3): 196-200.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: http://tougao.cdmc.edu.cn:8088/CN/

http://tougao.cdmc.edu.cn:8088/CN/Y2025/V42/I3/196

参考文献

[1] 张庆松,张榕,马珊珊,等. 具有PDZ结合基序的转录共激活因子对宫颈癌细胞的增殖、侵袭和迁移的影响[J]. 中国临床药理学杂志,2023,39(15):2217-2221.
[2] 王妤琪,唐晶晶,陈远远,等. 体素内不相干运动成像衍生参数与宫颈癌患者国际妇产科联盟分期的关系及对放疗疗效的评估价值分析[J]. 中国性科学,2023,32(4):84-88.
[3] Hernandez AL, Young CD, Bian L, et al.PARP Inhibition Enhances Radiotherapy of SMAD4-Deficient Human Head and Neck Squamous Cell Carcinomas in Experimental Models[J]. Clin Cancer Res, 2020, 26(12): 3058-3070.
[4] Vijayakumar G, Narwal A, Kamboj M, et al.Association of SOX2, OCT4 and WNT5A Expression in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma: An Immunohistochemical Study[J]. Head Neck Pathol, 2020, 14(3): 749-757.
[5] 陈粉合,邓祎,王恩栋,等. 宫颈癌患者HPV感染及EGFR、Sox2水平与预后的关系[J]. 实用癌症杂志,2020,35(10):1628-1630.
[6] 郝志晔,张晶,钱伟,等. 参芪排毒汤对宫颈癌组织人乳头瘤病毒E6/E7、P53和PRb基因蛋白表达的影响[J]. 陕西中医,2021,42(2):192-195,199.
[7] 解亦航,郭宇微,孙渤轩,等. 沉默E6相关蛋白对人乳头瘤病毒阴性宫颈癌细胞中p53蛋白表达水平的影响[J]. 中国生物制品学杂志,2023,36(2):163-165,171.
[8] 中国抗癌协会妇科肿瘤专业委员会. 宫颈癌诊断与治疗指南(第四版)[J]. 中国实用妇科与产科杂志,2018,34(6):613-622.
[9] 张娟,张栋栋,徐蕾,等. 转录因子NF-κB家族、STAT1、ELK1核表达对中晚期宫颈癌患者预后的评估价值[J]. 中国妇产科临床杂志,2022,23(1):28-30.
[10] 赵琳琳,胡春杨,刘倩. miR-155和SMAD4表达水平与宫颈癌放疗敏感性及预后的关系[J]. 热带医学杂志,2020,20(6):721-725.
[11] 刘澈. 宫颈癌患者血清Stathmin、Sox2及VEGF mRNA表达与淋巴结转移的相关性[J]. 转化医学杂志,2023,12(4):159-163.
[12] 邓蓉,张福云,雷福珍,等. 沉默长链非编码RNA HIFIA-AS2可抑制宫颈癌细胞的增殖、侵袭及迁移[J]. 南方医科大学学报,2020,40(11):1615-1621.
[13] Moshi JM, Ummelen M, Broers JLV, et al.SOX2 expression in the pathogenesis of premalignant lesions of the uterine cervix: its histo-topographical distribution distinguishes between low- and high-grade CIN[J]. Histochem Cell Biol, 2022, 158(6): 545-559.
[14] 宋殿芳,贾素敏,徐冲. LncRNA LINC00277通过与miR-27b结合调控p53表达抑制宫颈癌细胞的侵袭和迁移[J]. 中国老年学杂志,2021,41(18):4079-4083.
[15] 张珊,蔡广彦,范文文. 宫颈癌前病变患者组织中P53、CD44v6的表达异常与HPV感染的交互作用[J]. 中国优生与遗传杂志,2023,31(11):2244-2248.