Erastin对小鼠肝癌Hepa1-6细胞生长和转移的影响

承德医学院学报 ›› 2025, Vol. 42 ›› Issue (5): 373-377.

Erastin对小鼠肝癌Hepa1-6细胞生长和转移的影响

昌德炜¹, 徐淼¹, 汪碧宇¹, 李睿哲¹, 孙燕玲², 吕建国^3,*

1.湖北科技学院医学部药学院,湖北咸宁 437100;
2.湖北科技学院医学部基础医学院,湖北咸宁 437100;
3.湖北科技学院医学部临床医学院,湖北咸宁 437100

收稿日期:2024-08-20 出版日期:2025-10-10 发布日期:2025-10-23
通讯作者: *
基金资助:
湖北科技学院医学部口腔与眼视光医学院专项科研基金项目（2021WG10）

Effect of Erastin on The Growth and Metastasis of Hepatocellular Carcinoma Hepa1-6 Cells in Mice

CHANG De-wei¹, XU Miao¹, WANG Bi-yu¹, LI Rui-zhe¹, SUN Yan-ling², LV Jian-guo^3,*

1. School of Pharmacy, Hubei University of Science and Technology, Xianning Medical College, Xianning, Hubei, 437100, China;
2. School of Basic Medical Sciences, Hubei University of Science and Technology, Xianning Medical College, Xianning, Hubei, 437100, China;
3. School of Clinical Medical Sciences, Hubei University of Science and Technology, Xianning Medical College, Hubei University of Science and Technology, Xianning, Hubei, 437100, China

Received:2024-08-20 Online:2025-10-10 Published:2025-10-23

摘要/Abstract

摘要： 目的通过体外细胞实验探讨铁死亡诱导剂Erastin对小鼠肝癌Hepa1-6细胞生长和转移的影响。方法选取不同浓度Erastin作用于Hepa1-6细胞,用细胞增殖-毒性实验（MTT实验和CCK-8实验）检测Hepa1-6细胞的增殖和活性,平板克隆检测细胞的增殖能力,Transwell实验检测细胞的侵袭和迁移能力,划痕实验检测细胞的迁移能力。结果随着Erastin浓度的增加,Hepa1-6细胞的增殖抑制率逐渐上升,存活率和增殖能力下降,迁移和侵袭的细胞数减少,迁移率降低。结论 Erastin能显著抑制小鼠肝癌 Hepa1-6细胞的增殖活性,对Hepa1-6细胞的侵袭和迁移能力也有抑制作用,且存在剂量依赖性。

关键词: erastin, 肝癌, 增殖, 侵袭, 迁移

Abstract: Objective To investigate the effect of Erastin on the growth and metastasis of hepatocellular carcinoma Hepa1-6 cells in vitro. Methods Hepa1-6 cells were treated with Erastin at different concentrations. The proliferation and activity of Hepa1-6 cells were assessed using cell proliferation-cytotoxicity assays, specifically the MTT assay and the CCK-8 assay. The proliferative capacity of the cells was evaluated through the plate colony formation assay. Additionally, the invasive and migratory abilities of the cells were investigated using the Transwell assay, with the migratory ability further examined through the scratch assay. Results As the concentration of Erastin increased, the inhibition rate of proliferation in Hepa1-6 cells progressively rose, while the survival rate and proliferative capacity declined correspondingly. Additionally, there was a reduction in both the number of migrating and invading cells, leading to decreased cellular mobility. Conclusion Erastin significantly inhibits the proliferation of Hepa1-6 cells and also reduces their invasion and migration in a dose-dependent manner.

Key words: erastin, liver cancer, proliferation, invasion, migration

中图分类号:

昌德炜, 徐淼, 汪碧宇, 李睿哲, 孙燕玲, 吕建国. Erastin对小鼠肝癌Hepa1-6细胞生长和转移的影响[J]. 承德医学院学报, 2025, 42(5): 373-377.

CHANG De-wei, XU Miao, WANG Bi-yu, LI Rui-zhe, SUN Yan-ling, LV Jian-guo. Effect of Erastin on The Growth and Metastasis of Hepatocellular Carcinoma Hepa1-6 Cells in Mice[J]. Journal of Chengde Medical University, 2025, 42(5): 373-377.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: http://tougao.cdmc.edu.cn:8088/CN/

http://tougao.cdmc.edu.cn:8088/CN/Y2025/V42/I5/373

[1]	陈丽贞, 刘垠浩, 陈育青, 刘霏, 郭腾捷. Smurf2靶向EYA2对高糖诱导肾小球系膜细胞增殖和纤维化的影响[J]. 承德医学院学报, 2025, 42(5): 378-383.
[2]	周涛, 付溶川, 唐熙峻, 孙雨, 郑帅, 赵恩宏, 肖丽君. 沉默YTHDF3表达对HCT-116细胞增殖、迁移和凋亡的影响[J]. 承德医学院学报, 2025, 42(1): 1-6.
[3]	王云鹏, 高建步, 贾莹. 急性心肌梗死患者血清学指标水平与PCI术后预后的关系[J]. 承德医学院学报, 2024, 41(6): 471-474.
[4]	柳雪, 熊辉, 苏永志, 王杨洋, 王鹏, 陶亚东. hsa-circ-0001862对舌鳞癌细胞表型的影响研究[J]. 承德医学院学报, 2024, 41(3): 185-189.
[5]	韩思雨, 李雨欣, 易梦雅, 李警耀, 陈志宏. 丝胶靶向Akt1调控PI3K/Akt信号通路促进STZ致损伤INS-1细胞增殖[J]. 承德医学院学报, 2024, 41(2): 96-100.
[6]	苗亚琼, 郭黎英, 徐赛赛, 和谦. 血清PIVKA-Ⅱ、CHI3L1、TFF-1水平在原发性肝癌患者中的变化及临床意义[J]. 承德医学院学报, 2024, 41(1): 30-33.
[7]	马杉, 谢利德, 姚伟娟. Tmod1过表达对大鼠血管平滑肌细胞迁移和侵袭的影响[J]. 承德医学院学报, 2023, 40(6): 460-464.
[8]	雍金鹏, 周世繁, 周超锋, 郭志忠. 薯蓣丸加减方治疗气血两虚型肝癌癌因性疲乏的疗效观察[J]. 承德医学院学报, 2023, 40(6): 488-490.
[9]	王建平, 王小青, 赵学荣, 高亚贤, 杨岚, 肖丽君. lncRNA-AK093407通过EMT促进结直肠癌细胞增殖和转移[J]. 承德医学院学报, 2023, 40(5): 361-366.
[10]	王会杰, 孙珍贵, 赵文英, 耿彪. S100钙结合蛋白A16促进肺腺癌增殖和侵袭进展的研究[J]. 承德医学院学报, 2023, 40(5): 389-394.
[11]	林珏, 张园青, 黄莉. 维生素D₃对缺氧状态下成骨细胞增殖分化影响的研究[J]. 承德医学院学报, 2023, 40(4): 276-280.
[12]	王伟业, 尹威, 李泽同. 褪黑素对胰岛β细胞增殖与功能及p38 MAPK蛋白表达的影响[J]. 承德医学院学报, 2023, 40(3): 186-190.
[13]	胡维晟, 林嘉鑫, 卢楠, 黄建梅, 李明. IFITM3通过P38-MAPK/EMT信号轴对胃癌细胞的影响[J]. 承德医学院学报, 2023, 40(2): 95-100.
[14]	姚雪瑞, 党振, 孙雪, 忽书霞. 仙鹤草通过诱导肿瘤细胞凋亡和自噬抑制肝癌裸鼠移植瘤生长[J]. 承德医学院学报, 2022, 39(6): 460-464.
[15]	吴涛, 杨柳, 聂山茂, 张杨, 张鑫. 长链非编码RNA SNHG16通过miR-141-3p/FOXJ3通路调控肝细胞癌的增殖[J]. 承德医学院学报, 2022, 39(4): 271-276.