臭椿酮对非小细胞肺癌顺铂耐药细胞A549/DDP的增敏作用

承德医学院学报 ›› 2023, Vol. 40 ›› Issue (3): 181-186.

• 基础医学 • 下一篇

臭椿酮对非小细胞肺癌顺铂耐药细胞A549/DDP的增敏作用

李祥伶¹, 刘承一², 刘镭¹, 陈龙¹, 于胜利³, 许倩^1,*

1.承德医学院基础医学院,河北承德 067000;
2.承德医学院附属医院,河北承德 067000;
3.承德市生态环境检验监测站,河北承德 067000

收稿日期:2022-10-12 出版日期:2023-06-10 发布日期:2023-09-15
通讯作者: *
基金资助:
国家自然科学基金项目（81703001）; 河北省重点研发计划项目（19277783D）; 河北省自然科学基金资助项目（H2021406021）; 河北省医学科学研究计划项目（20210247; 20221335）; 承德医学院重大项目科研专项（KY2020005）; 政府资助临床医学优秀人才培养项目; 河北省高校重点学科建设[冀教高（2013）4号]

Ailanthone Sensitizes Resistant Cell Line A549/DDP to Cisplatinin Non-small Cell Lung Cancer

LI Xiang-ling¹, LIU Cheng-yi², LIU Lei¹, CHEN Long¹, YU Sheng-li³, XU Qian^1,*

1. School of Basic Medicine, Chengde Medical University, Chengde, Hebei, 067000, China;
2. Affiliated Hospital of Chengde Medical University, Chengde, Hebei, 067000, China;
3. Chengde Ecological environment inspection and monitoring Station, Chengde, Hebei, 067000, China

Received:2022-10-12 Online:2023-06-10 Published:2023-09-15

摘要/Abstract

摘要： 目的探讨臭椿酮（AIL）对非小细胞肺癌顺铂耐药细胞系A549/DDP的增敏作用。方法采用MTT检测AIL对A549和A549/DDP细胞活力的影响;Chou-Talalay中效法分析臭椿酮与顺铂的联合用药指数（CI）;流式细胞术检测细胞周期及凋亡,Western blotting法检测凋亡相关蛋白表达水平。结果 AIL（0.6μmol/L）与DDP（50μg/mL）联合用药有协同作用;细胞周期阻滞在G1期,同时细胞凋亡率升高;Caspase-3和 Bcl-2蛋白表达下降,Cleaved Caspase3、Bax、CDK4和CyclinD1的蛋白表达升高。结论 AIL可增强A549/DDP细胞对DDP的敏感性,协同DDP抑制A549/DDP细胞的生长,促进其凋亡。

关键词: 臭椿酮, 顺铂, 非小细胞肺癌, A549/DDP细胞, 增敏

Abstract: Objective To investigate the sensitization effect of Ailanthone (AIL) on cisplatin-resistant A549/DDP cells of non-small cell lung cancer. Methods The effects of AIL on the viability of A549 and A549/DDP cells were detected by MTT. Analysis of the combined drug use index(CI) of Ailanone and cisplatin using Chou-Talalay. Cell cycle and apoptosis were detected by flow cytometry, and the expression of apoptosis-related proteins were detected by Western blotting. Results AIL (0.6μmol/L) and DDP (50μg/mL) had synergistic effect. Cell cycle arrest and apoptosis rate increased in G1 phase. The protein expression of caspase-3 and bcl-2 decreased, but the protein expression of Cleaved Caspase3, Bax, CDK4 and CyclinD1 increased. Conclusion AIL enhanced the sensitivity of DDP to A549/DDP, and increased the growth inhibition and apoptosis of DDP on A549/DDP.

Key words: ailanthone, cisplatin, non-small cell lung cancer, A549/DDPcells, sensibilization

中图分类号:

R285

李祥伶, 刘承一, 刘镭, 陈龙, 于胜利, 许倩. 臭椿酮对非小细胞肺癌顺铂耐药细胞A549/DDP的增敏作用[J]. 承德医学院学报, 2023, 40(3): 181-186.

LI Xiang-ling, LIU Cheng-yi, LIU Lei, CHEN Long, YU Sheng-li, XU Qian. Ailanthone Sensitizes Resistant Cell Line A549/DDP to Cisplatinin Non-small Cell Lung Cancer[J]. Journal of Chengde Medical University, 2023, 40(3): 181-186.

参考文献

[1] Cao M, Li H, Sun D.Cancer burden of major cancers in China: A need for sustainable actions[J]. Cancer Commun (Lond), 2020, 40(5): 205-210.
[2] Wu H, Mu X, Liu L, et al.Bone marrow mesenchymal stem cells-derived exosomal microRNA-193a reduces cisplatin resistance of non-small cell lung cancer cells via targeting LRRC1[J]. Cell Death Dis, 2020, 11(9): 801.
[3] Meng J, Chang C, Chen Y, et al.EGCG overcomes gefitinib resistance by inhibiting autophagy and augmenting cell death through targeting ERK phosphorylation in NSCLC[J]. Onco Targets Ther, 2019, 12: 6033-6043.
[4] Miller KD, Siegel RL, Lin CC, et al.Cancer treatment and survivorship statistics, 2016[J]. CA Cancer J Clin, 2016, 66(4): 271-289.
[5] Panneerselvam J, Mohandoss P, Patel R, et al.DCLK1 Regulates Tumor Stemness and Cisplatin Resistance in Non-small Cell Lung Cancer via ABCD-Member-4[J]. Mol Ther Oncolytics, 2020, 18: 24-36.
[6] Chen L, Wu C, Wang H, et al.Analysis of Long Noncoding RNAs in Aila-Induced Non-Small Cell Lung Cancer Inhibition[J]. Front Oncol, 2021, 11: 652567.
[7] Zhuo Z, Hu J, Yang X, et al.Ailanthone Inhibits Huh7 Cancer Cell Growth via Cell Cycle Arrest and Apoptosis In Vitro and In Vivo[J]. Sci Rep, 2015, 5: 16185.
[8] 魏立璇,孙涛,马艳菊,等. 臭椿酮抑制ENST00000441270/miR-149轴影响结直肠癌细胞增殖和凋亡的实验研究[J]. 中华实验外科杂志,2021,38(2):230-234.
[9] Chen L, Wu C, Wang H, et al.Analysis of Long Noncoding RNAs in Aila-Induced Non-Small Cell Lung Cancer Inhibition[J]. Front Oncol, 2021, 11: 652567.
[10] 皇甫娟,张强,魏祯瑶,等. 基于p53介导自噬通路探讨臭椿酮对顺铂耐药胃癌细胞株耐药性的影响[J].现代药物与临床,2021,36(6):1112-1118.
[11] Chou TC.Drug combination studies and their synergy quantification using the Chou-Talalay method[J].Cancer Res, 2010, 70(2): 440-446.
[12] Liao XZ, Gao Y, Sun LL, et al.Rosmarinic acid reverses non-small cell lung cancer cisplatin resistance by activating the MAPK signaling pathway[J]. Phytother Res, 2020, 34(5): 1142-1153.
[13] Sun CY, Zhu Y, Li XF, et al.Scutellarin Increases Cisplatin-Induced Apoptosis and Autophagy to Overcome Cisplatin Resistance in Non-small Cell Lung Cancer via ERK/p53 and c-met/AKT Signaling Pathways[J]. Front Pharmacol, 2018, 9: 92.
[14] Fan CC, Tsai ST, Lin CY, et al.EFHD2 contributes to non-small cell lung cancer cisplatin resistance by the activation of NOX4-ROS-ABCC1 axis[J]. Redox Biol, 2020, 34: 101571.
[15] Yu N, Xiong Y, Wang C.Bu-Zhong-Yi-Qi Decoction, the Water Extract of Chinese Traditional Herbal Medicine, Enhances Cisplatin Cytotoxicity in A549/DDP Cells through Induction of Apoptosis and Autophagy[J]. Biomed Res Int, 2017, 3692797.
[16] 王浩,段佩雯,伏杰,等. 中药单体调控自噬逆转肿瘤耐药的实验研究现状[J]. 中医药导报,2019,25(13):53-56.
[17] 田艳,冼乐武. 臭椿酮对非小细胞肺癌细胞生物学特性的影响及机制研究[J]. 河北中医,2019,41(2):268-274.
[18] Yu W, Zhang X, Zhang W, et al.19-Hydroxybufalin inhibits non-small cell lung cancer cell proliferation and promotes cell apoptosis via the Wnt/β-catenin pathway[J]. Exp Hematol Oncol, 2021, 10(1): 48.
[19] Wang R, Lu Y, Li H, et al.Antitumor activity of the Ailanthus altissima bark phytochemical ailanthone against breast cancer MCF-7 cells[J]. Oncol Lett, 2018;15(4): 6022-6028.
[20] Yang X, Zheng F, Xing H, et al.Resistance to chemotherapy-induced apoptosis via decreased caspase-3 activity and overexpression of antiapoptotic proteins in ovarian cancer[J]. Cancer Res Clin Oncol, 2004, 130(7): 423-428.
[21] 银瑞,董新伟,王铮. 顺铂诱导食管癌EC9706耐药细胞中Bcl-2的表达[J]. 中国老年学杂志,2016,36(3):562-564.
[22] Chami M, Prandini A, Campanella M, et al.Bcl-2 and Bax exert opposing effects on Ca2+ signaling, which do not depend on their putative pore-forming region[J]. Biol Chem, 2004, 279(52): 54581-54589.

臭椿酮对非小细胞肺癌顺铂耐药细胞A549/DDP的增敏作用

Ailanthone Sensitizes Resistant Cell Line A549/DDP to Cisplatinin Non-small Cell Lung Cancer

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价

[1]	郭娜, 徐颖, 李祥伶, 王鹏, 李琳, 许倩, 刘镭. 基于生物信息学分析miR-125b-5p在肺腺癌中的表达及预后价值[J]. 承德医学院学报, 2022, 39(5): 365-370.
[2]	张庆丽, 张筠, 伍爱荣, 刘德勇. 厄洛替尼对EGFR基因不同突变型NSCLC患者疗效及生存率的影响^*[J]. 承德医学院学报, 2019, 36(3): 202-204.
[3]	薛晶雯, 王又君, 郭静, 吕喜英. 非小细胞肺癌脑转移的治疗进展[J]. 承德医学院学报, 2019, 36(1): 65-68.
[4]	郭静, 王又君, 薛晶雯, 吕喜英. 培美曲塞联合铂类与其它铂二联方案治疗晚期肺腺癌临床疗效比较[J]. 承德医学院学报, 2018, 35(6): 472-475.