沉默YTHDF3表达对HCT-116细胞增殖、迁移和凋亡的影响

承德医学院学报 ›› 2025, Vol. 42 ›› Issue (1): 1-6.

• 基础医学 • 下一篇

沉默YTHDF3表达对HCT-116细胞增殖、迁移和凋亡的影响

周涛¹, 付溶川¹, 唐熙峻¹, 孙雨¹, 郑帅², 赵恩宏², 肖丽君^1,*

1.承德医学院基础医学院,河北承德 067000;
2.承德医学院附属医院,河北承德 067000

收稿日期:2024-06-24 出版日期:2025-02-10 发布日期:2025-02-24
通讯作者: *

The Effect of Silencing the Expression of YTHDF3 on the Proliferation, Migration, and Apoptosis of HCT-116 Cells

ZHOU Tao FU Rong-chuan TANG Xi-jun SUN Yu ZHENG Shuai ZHAO En-hong XIAO Li-jun ZHOU Tao¹, FU Rong-chuan¹, TANG Xi-jun¹, SUN Yu¹, ZHENG Shuai², ZHAO En-hong², XIAO Li-jun^1,*

1. Basic Medical Institute of Chengde Medical University, Chengde, Hebei, 067000, China;
2. Affiliated Hospital of Chengde Medical University, Chengde, Hebei, 067000, China

Received:2024-06-24 Online:2025-02-10 Published:2025-02-24

摘要/Abstract

摘要： 目的探究YTH N6甲基腺苷RNA结合蛋白3（YTHDF3）对人结直肠癌细胞系HCT-116增殖、迁移和凋亡的影响。方法将细胞分为阴性对照组、干扰组1、干扰组2、干扰组3,在结直肠癌细胞HCT-116中转染小干扰YTHDF3,通过qRT-PCR和Western blot实验检测各组细胞YTHDF3表达。采用CCK-8实验、平板克隆实验检测各组细胞增殖能力,Transwell实验和划痕实验检测各组细胞迁移能力,流式细胞术检测各组细胞凋亡能力。结果通过qRT-PCR和Western blot实验验证HCT-116细胞沉默效率。与阴性对照组相比,在HCT-116细胞中转染小干扰YTHDF3后,干扰组2和干扰组3能有效降低YTHDF3的表达;沉默YTHDF3能显著抑制HCT-116细胞增殖和迁移,促进细胞凋亡。结论沉默YTHDF3能够减缓HCT-116细胞增殖和迁移,促进HCT-116细胞凋亡。

关键词: 结直肠癌, YTHDF3, HCT-116, 增殖, 迁移, 凋亡

Abstract: Objective To investigate the impact of YTH N6 methyladenosine RNA binding protein 3 (YTHDF3) on the proliferation, migration, and apoptosis of human colorectal cancer cell line HCT-116. Methods The cells were divided into a negative control group, interference groups 1, 2, and 3. Small interfering RNA targeting YTHDF3 was transfected into colorectal cancer cells HCT-116. The expression of YTHDF3 in each group was assessed using qRT-PCR and Western blot analysis. Cell proliferation was evaluated using CCK-8 assay and plate cloning assay, while cell migration was assessed using transwell assay and scratch assay. Flow cytometry was used to measure cell apoptosis. Results The silencing efficiency of HCT-116 cells was validated through qRT-PCR and Western blot analysis. Transfection of small interfering YTHDF3 effectively reduced the expression of YTHDF3 in HCT-116 cells compared to the negative control group. Silencing YTHDF3 can significantly inhibited cell proliferation and migration while promoting apoptosis in HCT-116 cells. Conclusion Silencing YTHDF3 can decelerates cell proliferation and migration while enhancing apoptosis in HCT-116 cells.

Key words: Colorectal cancer, YTHDF3, HCT-116, Proliferation, Migration, Apoptosis

中图分类号:

R735.3

周涛, 付溶川, 唐熙峻, 孙雨, 郑帅, 赵恩宏, 肖丽君. 沉默YTHDF3表达对HCT-116细胞增殖、迁移和凋亡的影响[J]. 承德医学院学报, 2025, 42(1): 1-6.

ZHOU Tao, FU Rong-chuan, TANG Xi-jun, SUN Yu, ZHENG Shuai, ZHAO En-hong, XIAO Li-jun. The Effect of Silencing the Expression of YTHDF3 on the Proliferation, Migration, and Apoptosis of HCT-116 Cells[J]. Journal of Chengde Medical University, 2025, 42(1): 1-6.

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http://tougao.cdmc.edu.cn:8088/CN/Y2025/V42/I1/1

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沉默YTHDF3表达对HCT-116细胞增殖、迁移和凋亡的影响

The Effect of Silencing the Expression of YTHDF3 on the Proliferation, Migration, and Apoptosis of HCT-116 Cells

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