下丘脑室旁核内GABAA受体活性对小鼠焦虑样行为的影响

承德医学院学报 ›› 2025, Vol. 42 ›› Issue (3): 181-186.

• 基础医学 • 下一篇

下丘脑室旁核内GABA_A受体活性对小鼠焦虑样行为的影响

李颜君¹, 郜慧英¹, 张娜娜^2,*

1.菏泽医学专科学校护理系,山东菏泽 274000;
2.青岛大学附属医院检验科,山东青岛 266003

收稿日期:2024-06-27 出版日期:2025-06-10 发布日期:2025-06-18
通讯作者: *

Effects of GABA_AReceptor in the Periventricular Nucleus of Hypothalamus on Anxiety-like Behavior in Mice

LI Yan-jun¹, GAO Hui-ying¹, ZHANG Na-na^2,*

1. Department of nursing, Heze Medical College, Heze, Shandong, 274000, China;
2. Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China

Received:2024-06-27 Online:2025-06-10 Published:2025-06-18

摘要/Abstract

摘要： 目的观察下丘脑室旁核（PVN）内GABA_A受体活性对小鼠焦虑样行为的影响。方法随机选取3只正常KM小鼠,荧光免疫组织化学法观察下丘脑PVN内GABA_A受体表达;随机选取24只正常KM小鼠,按下丘脑PVN内注射不同药物分为生理盐水（NS）组（0.3 μL）、GABA_A受体激动剂Isoguvacine（Isog,0.3 μL）组或GABA_A受体拮抗剂Bicuculline（BIC,0.3 μL）组,每组8只。观察各组小鼠在PVN微量注射不同药物后的焦虑样行为变化。结果小鼠下丘脑PVN内有GABA_A受体表达;旷场实验、高十字架迷宫实验、埋珠实验结果均显示,PVN内微量注射Isog,小鼠没有出现焦虑样行为（P>0.05）,而PVN内微量注射BIC,促进了小鼠的焦虑状态（P<0.05）。结论小鼠PVN内的GABA_A受体活性可能与抗焦虑作用有关。

关键词: 下丘脑室旁核, GABA_A受体, 焦虑

Abstract: Objective To observe the effects of GABA_A receptor in the paraventricular nucleus (PVN) on anxiety-like behavior in mice. Methods Three normal KM mice were randomly selected to observe the expression of GABA_A receptors in the PVN using fluorescence immunohistochemistry. Additionally, twenty-four normal KM mice were randomly selected and divided into three groups based on the microinjection of different drugs into the PVN: Normal saline (NS) group (0.3 μL), GABA_A receptor agonist Isoguvacine (Isog) group (0.3 μL), and GABA_A receptor antagonist Bicuculline (BIC) group (0.3 μL), with 8 mice in each group. The anxiety-like behavior changes of the mice after microinjection of different drugs in the PVN were observed. Results GABA_A receptors were expressed in the PVN of mice. Results from open field, elevated plus maze, and marble burying tests showed that microinjection of GABA_A receptor agonist into the PVN did not induce anxiety-like behavior in mice (P>0.05), while microinjection of a GABA_A receptor antagonist promoted anxiety-like behavior (P<0.05). Conclusion The activity of GABA_A receptors within the PVN may be associated with anxiolytic effects in mice.

Key words: Periventricular nucleus of the hypothalamus, GABA_A receptors, Anxiety

中图分类号:

R749.72

李颜君, 郜慧英, 张娜娜. 下丘脑室旁核内GABA_A受体活性对小鼠焦虑样行为的影响[J]. 承德医学院学报, 2025, 42(3): 181-186.

LI Yan-jun, GAO Hui-ying, ZHANG Na-na. Effects of GABA_AReceptor in the Periventricular Nucleus of Hypothalamus on Anxiety-like Behavior in Mice[J]. Journal of Chengde Medical University, 2025, 42(3): 181-186.

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http://tougao.cdmc.edu.cn:8088/CN/Y2025/V42/I3/181

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下丘脑室旁核内GABA_A受体活性对小鼠焦虑样行为的影响

Effects of GABA_AReceptor in the Periventricular Nucleus of Hypothalamus on Anxiety-like Behavior in Mice

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下丘脑室旁核内GABAA受体活性对小鼠焦虑样行为的影响

Effects of GABAA Receptor in the Periventricular Nucleus of Hypothalamus on Anxiety-like Behavior in Mice

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下丘脑室旁核内GABA_A受体活性对小鼠焦虑样行为的影响

Effects of GABA_AReceptor in the Periventricular Nucleus of Hypothalamus on Anxiety-like Behavior in Mice