丝胶对链脲佐菌素作用下大鼠INS-1细胞Bax表达的调控作用*

承德医学院学报 ›› 2019, Vol. 36 ›› Issue (5): 364-367.

丝胶对链脲佐菌素作用下大鼠INS-1细胞Bax表达的调控作用^*

史硕¹, 王芳芳², 葛婷¹, 阮鸿娇¹, 陈志宏^1△, 宋成军^1△

1.承德医学院人体解剖学教研室,河北承德 067000;
2.承德医学院附属医院

收稿日期:2019-04-11 出版日期:2019-10-10 发布日期:2021-11-18
通讯作者: △
基金资助:
* 国家自然科学基金资助项目（81441133）; 河北省自然科学基金资助项目（H2013406096）; 河北省教育厅青年基金项目（QN20131089）; 河北省高校重点发展学科“人体解剖与组织胚胎学”建设项目资助

REGULATORY EFFECTS OF SERICIN ON BAX EXPRESSION IN RAT INS-1 CELL INDUCED BY STREPTOZOTOCIN

Department of Human Anatomy, Chengde Medical College, Hebei Chengde 067000, China

Received:2019-04-11 Online:2019-10-10 Published:2021-11-18

摘要/Abstract

摘要： 目的:观察丝胶对链脲佐菌素（STZ）致损伤大鼠胰岛素瘤细胞（INS-1细胞）Bax表达的影响。方法:INS-1细胞分为三组,正常对照组（不予任何药物处理）、STZ处理组（10mmol/L的STZ处理细胞24h）、丝胶保护组（10mmol/L的STZ、300μg/ml的丝胶共同处理细胞24h）。采用CCK-8法观察各组细胞的活力,蛋白印迹和实时荧光定量PCR法检测各组细胞Bax蛋白和mRNA的表达情况。结果:STZ处理组INS-1细胞的活力明显低于正常对照组、Bax蛋白和mRNA表达明显高于正常对照组（P<0.05）;丝胶保护组INS-1细胞的活力明显高于STZ处理组、Bax蛋白和mRNA表达明显低于STZ处理组（P<0.05）。结论:丝胶对STZ致损伤大鼠INS-1细胞具有保护作用,其机制可能与下调Bax的表达有关。

关键词: 丝胶, 大鼠胰岛素瘤细胞（INS-1细胞）, 链脲佐菌素（STZ）, Bax, 糖尿病

Abstract: Objective: To observe the effects of sericin on Bax expression in rat insulinoma cell (INS-1 cell) injured by streptozotocin (STZ). Methods: INS-1 cells were divided into 3 groups: normal control group, the cells were not treated with any drugs; STZ treatment group, the cells were treated with STZ (10mmol/L) for 24h; sericin protection group, the cells were treated with STZ (10mmol/L) and sericin (300μg/ml) for 24h. CCK8 assay was used to measure the viability of INS-1 cells in each group; Western blot and real-time fluorescence quantitative PCR were used to detect the Bax protein and mRNA expression in INS-1 cells. Results: The viability of INS-1 cells in STZ treatment group was remarkably lower than normal control group, while the Bax protein and mRNA expression were remarkably higher (P<0.05). The viability of INS-1 cells in sericin treatment group was remarkably higher than STZ treatment group, while the Bax protein and mRNA expression were remarkably lower (P<0.05). Conclusions: Sericin has protective effects on INS-1 cells injured by STZ, the mechanisms maybe concerned with it can down regulate Bax expression.

Key words: Sericin, Rat insulinoma cell (INS-1 cell), Streptozotocin (STZ), Bax, Diabetes mellitus

中图分类号:

R587.1

史硕, 王芳芳, 葛婷, 阮鸿娇, 陈志宏, 宋成军. 丝胶对链脲佐菌素作用下大鼠INS-1细胞Bax表达的调控作用^*[J]. 承德医学院学报, 2019, 36(5): 364-367.

SHI Shuo, WANG Fang-fang, GE Ting, et al. REGULATORY EFFECTS OF SERICIN ON BAX EXPRESSION IN RAT INS-1 CELL INDUCED BY STREPTOZOTOCIN[J]. Journal of Chengde Medical University, 2019, 36(5): 364-367.

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