miR-15a-5p和MDR1在大肠癌组织中的表达及临床意义*

承德医学院学报 ›› 2019, Vol. 36 ›› Issue (6): 455-458.

miR-15a-5p和MDR1在大肠癌组织中的表达及临床意义^*

黄艳洁, 袁亚男, 李璐, 许倩, 郑纪宁^△

承德医学院,河北承德 067000

收稿日期:2019-05-07 出版日期:2019-12-10 发布日期:2021-11-18
通讯作者: △
基金资助:
* 河北省高校省级重点学科建设项目（冀教高（2013）4号病理学与病理生理学）

EXPRESSION AND CLINICAL SIGNIFICANCE OF MIR-15A-5P AND MDR1 MRNA IN COLORECTAL CANCER

<a href="http://tougao.cdmc.edu.cn:8088/EN/article/advancedSearchResult.do?searchSQL=(((HUANG Yan-jie[Author]) AND 1[Journal]) AND year[Order])" target="_blank">HUANG Yan-jie</a>, <a href="http://tougao.cdmc.edu.cn:8088/EN/article/advancedSearchResult.do?searchSQL=(((YUAN Ya-nan[Author]) AND 1[Journal]) AND year[Order])" target="_blank">YUAN Ya-nan</a>, <a href="http://tougao.cdmc.edu.cn:8088/EN/article/advancedSearchResult.do?searchSQL=(((LI Lu[Author]) AND 1[Journal]) AND year[Order])" target="_blank">LI Lu</a>, <a href="http://tougao.cdmc.edu.cn:8088/EN/article/advancedSearchResult.do?searchSQL=(((et al[Author]) AND 1[Journal]) AND year[Order])" target="_blank">et al</a>

Chengde Medical College, Hebei Chengde 067000, China

Received:2019-05-07 Online:2019-12-10 Published:2021-11-18

摘要/Abstract

摘要： 目的:研究miR-15a-5p和多药耐药基因1（MDR1）mRNA在大肠癌组织中的表达及意义。方法:采用实时荧光定量PCR法检测44例大肠癌组织及对应的癌旁正常肠黏膜组织中miR-15a-5p、MDR1 mRNA的表达情况,分析miR-15a-5p、MDR1 mRNA表达与大肠癌临床病理特征的关系,以及大肠癌组织中miR-15a-5p与MDR1 mRNA表达的相关性。结果:大肠癌组织miR-15a-5p的相对表达量明显低于癌旁正常组织,MDR1 mRNA的相对表达量明显高于癌旁正常组织(P<0.05)。大肠癌组织miR-15a-5p、MDR1 mRNA表达均与TNM分期和淋巴结转移有关(P<0.05)。Spearman相关分析显示,大肠癌组织miR-15a-5p表达与MDR1 mRNA表达呈负相关关系(r=-0.343,P<0.05)。结论:大肠癌组织中miR-15a-5p呈低表达、MDR1呈高表达,二者可能在大肠癌的发生发展过程中发挥重要作用。

关键词: 大肠癌, miR-15a-5p, 多药耐药基因1（MDR1）

Abstract: Objective: To study the expression and clinical significance of miR-15a-5p and multidrug resistance gene 1(MDR1) mRNA in colorectal carcinoma tissues. Methods: RT-qPCR was used to detect the expression of miR-15a-5p and MDR1 mRNA in 44 cases of colorectal carcinoma and adjacent non-tumor tissues. The relationships between miR-15a-5p, MDR1 mRNA and clinical pathological features of colorectal carcinoma, as well as the correlations between miR-15a-5p and MDR1 mRNA in colorectal carcinoma were also analyzed. Results: Compared with the adjacent non-tumor tissues, miR-15a-5p mRNA expression was significantly lower in colorectal carcinoma, but MDR1 mRNA expression was significantly higher (P<0.05). The miR-15a-5p and MDR1 mRNA expression were all concerned with lymph node metastasis and TNM stage of colorectal carcinoma (P<0.05). In colorectal carcinoma, miR-15a-5p mRNA expression was negatively correlated with MDR1 mRNA expression (r=-0.343, P<0.05). Conclusions: The expression of miR-15a-5p was low and MDR1 was high in colorectal cancer tissues. Both of them may play an important role in development of colorectal carcinoma.

Key words: Colorectal carcinoma, miR-15a-5p, Multidrug resistance 1 gene (MDR1)

中图分类号:

R735.3

黄艳洁, 袁亚男, 李璐, 许倩, 郑纪宁. miR-15a-5p和MDR1在大肠癌组织中的表达及临床意义^*[J]. 承德医学院学报, 2019, 36(6): 455-458.

HUANG Yan-jie, YUAN Ya-nan, LI Lu, et al. EXPRESSION AND CLINICAL SIGNIFICANCE OF MIR-15A-5P AND MDR1 MRNA IN COLORECTAL CANCER[J]. Journal of Chengde Medical University, 2019, 36(6): 455-458.

参考文献

[1] BRAY F, FERLAY J, JEMAL A, et al.Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018, 68(6): 394-424.
[2] LENG J, SONG Q, ZHAO Y, et al.miR-15a represses cancer cell migration and invasion under conditions of hypoxia by targeting and downregulating Bcl-2 expression in human osteosarcoma cells[J]. Int J Oncol, 2018, 52(4): 1095-1104.
[3] WANG T, HOU J, LI Z, et al.miR-15a-3p and miR-16-1-3p negatively regulate Twist1 to repress gastric cancer cell invasion and metastasis[J]. Int J Biol Sci, 2017, 13(1): 122-134.
[4] FAN B, CHEN L P, YUAN Y H, et al.MiR-15a-3p suppresses the growth and metastasis of ovarian cancer cell by targeting Twist1[J]. Eur Rev Med And Pharmacol Sci, 2019, 23(5): 1934-1946.
[5] CHUFAN E E, SIM H M, AMUBUDKAR S V.Molecular basis of the polyspecificity of P-glycoprotein(ABCB1): recent biochemical and structural studies[J]. Adv Cancer Res, 2015, 125: 71-96.
[6] 王琳. LINC00473通过miR-15a-5p促进结肠癌对紫杉醇的耐药[D].广州:南方医科大学,2018.
[7] GUO S, XU X, TANG Y, et al.Mir-15a inhibits cell proliferation and epithelial to mesenchymal transition in pancreatic ductal adenocarcinoma by down-regulating Bmi-1 expression[J]. Cancer Lett, 2014, 344(1): 40-46.
[8] BOZOK ÇETINTAS V, TETIK VARDARLι A, DÜZGÜN Z, et al. miR-15a enhances the anticancer effects of cisplatin in the resistant non-small cell lung cancer cells[J]. Tumour Biol, 2016, 37(2): 1739-1751.
[9] ALDERMAN C, SEHLAOUI A, XIAO Z, et al.MicroRNA-15a inhibits the growth and invasiveness of malignant melanoma and directly targets on CDCA4 gene[J]. Tumour Biol, 2016, 37(10): 13941-13950.
[10] 郭正扬. microRNA-15a在胃癌中的表达及其作用机制研究[D].上海:复旦大学,2011.
[11] 刘玺章,王代友.优化P-gp给药模式的相关研究进展[J].广西医学,2015,37(2):232-234,238.
[12] 丛熙,樊建慧.P-糖蛋白对结肠癌多药耐药的影响[J].中国生物化学与分子生物学报,2019,35(1):19-26.
[13] SHAIN K H, DALTON W S.Cell adhesion is a key determinant in de novo multidrug resistance(MDR): new targets for the prevention of acquired MDR[J]. Mol Cancer Ther, 2001, 1(1): 69-78.