鬼臼苦素对人乳腺癌MCF-7细胞增殖的抑制作用

承德医学院学报 ›› 2020, Vol. 37 ›› Issue (1): 5-7.

鬼臼苦素对人乳腺癌MCF-7细胞增殖的抑制作用

刘赛璇, 薛晶, 周丽丽, 赵杨, 申兴斌^△

承德医学院附属医院病理科,河北承德 067000

收稿日期:2019-07-24 出版日期:2020-02-10 发布日期:2021-11-19
通讯作者: △

INHIBITING EFFECTS OF PICROPODOPHYLLIN ON HUMAN BREAST CANCER MCF-7 CELL PROLIFERATION

Department of Pathology, the Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China

Received:2019-07-24 Online:2020-02-10 Published:2021-11-19

摘要/Abstract

摘要： 目的探讨鬼臼苦素对人乳腺癌MCF-7细胞增殖的抑制作用。方法体外培养人乳腺癌MCF-7细胞,采用MTT法检测不同浓度鬼臼苦素（0.1μmol/L、0.2μmol/L、0.4μmol/L、0.8μmol/L、1.6μmol/L、3.2μmol/L、6.4μmol/L）分别作用24h、48h、72h对MCF-7细胞增殖的抑制作用;同时,在倒置显微镜下观察不同浓度鬼臼苦素作用48h时MCF-7细胞形态结构的变化。结果浓度范围在0.1μmol/L~6.4μmol/L,随着鬼臼苦素药物浓度的增加及作用时间的延长,MCF-7细胞的生长抑制率逐渐升高,呈现一定的剂量-时间依赖关系（P<0.05）。鬼臼苦素作用MCF-7细胞24h、48h、72h的IC₅₀值分别为7.32μmol/L、0.720μmol/L、0.318μmol/L。倒置显微镜下观察,鬼臼苦素作用48h后MCF-7细胞回缩、结构不清、折光性降低。结论鬼臼苦素对人乳腺癌MCF-7细胞具有明显的抑制作用。

关键词: 鬼臼苦素, MCF-7细胞, 乳腺癌, 细胞增殖

Abstract: Objective: To investigate the inhibiting effects of picropodophyllin on human breast cancer MCF-7 cell proliferation. Methods: Human breast cancer MCF-7 cells were cultured in vitro, and MTT method was used to detect the inhibitory effects of different concentrations of picropodophyllin (0.1μmol/L, 0.2μmol/L, 0.4μmol/L, 0.8μmol/L, 1.6μmol/L,3.2μmol/L, 6.4μmol/L) on proliferation of MCF-7 cells for 24h, 48h and 72h. At the same time, the structural changes of MCF-7 cells were observed under inverted microscope at different concentrations of picropodophyllin for 48h. Results: The concentration range was 0.1μmol/L~ 6.4μmol/L; and the growth inhibiting rate of MCF-7 cells increased gradually with the increasing of picropodophyllin concentration and action time (P<0.05). The IC₅₀ of MCF-7 cells treated with picropodophyllin for 24h, 48h and 72h were 7.32μmol/L, 0.720μmol/L and 0.318μmol/L respectively. Under inverted microscope, the MCF-7 cells contracted with unclear structure and the refractivity reduced after treated with picropodophyllin for 48h. Conclusions: Picropodophyllin has significant inhibiting effects on human breast cancer MCF-7 cells.

Key words: Picropodophyllin, MCF-7 cells, Breast cancer, Cell proliferation

中图分类号:

R735.3

刘赛璇, 薛晶, 周丽丽, 赵杨, 申兴斌. 鬼臼苦素对人乳腺癌MCF-7细胞增殖的抑制作用[J]. 承德医学院学报, 2020, 37(1): 5-7.

LIU Sai-xuan, XUE Jing, ZHOU Li-li, et al. INHIBITING EFFECTS OF PICROPODOPHYLLIN ON HUMAN BREAST CANCER MCF-7 CELL PROLIFERATION[J]. Journal of Chengde Medical University, 2020, 37(1): 5-7.

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