益心酮片HPLC指纹图谱的构建*

承德医学院学报 ›› 2018, Vol. 35 ›› Issue (1): 6-8.

益心酮片HPLC指纹图谱的构建^*

徐宝欣, 杜义龙, 潘海峰, 李艳荣^△

承德医学院中药研究所/河北省中药研究与开发重点实验室,河北承德 067000

收稿日期:2017-04-28 出版日期:2018-02-10 发布日期:2021-11-21
通讯作者: △
基金资助:
* 河北省高等学校科学技术研究重点项目（ZD2015097）,河北省中医药管理局项目（2014063）,河北省高校重点学科建设项目资助

CONSTRUCTION OF HPLC FINGERPRINT OF YIXINTONG TABLETS

XU Bao-xin, DU Yi-long, PAN Hai-feng, et al

Institute of Chinese Materia Medica/Hebei Key Laboratory of Research and Exploitation of Traditional Chinese Medicine, Chengde Medical College, Hebei Chengde 067000, China

Received:2017-04-28 Online:2018-02-10 Published:2021-11-21

摘要/Abstract

摘要： 目的: 构建益心酮片的HPLC指纹图谱。方法: 采用HPLC法建立13批益心酮片的指纹图谱。色谱条件：ZORBAX SB-C₁₈色谱柱（5µm,4.6mm×250mm）,流动相为A：0.1%甲酸-水混合溶液、B：乙腈、C：四氢呋喃,梯度洗脱,流速为1.0ml/min,检测波长350nm,柱温30℃,进样量10µl。使用中药指纹图谱相似度评价软件（版本2004 A）进行相似度分析。结果: 根据13批益心酮片的指纹图谱检测结果,共标定出10个共有峰,13批益心酮片的相似度均大于0.99。结论: 该方法操作简单、可靠,重复性强,结果比较稳定,适用于益心酮片的质量评价。

关键词: 益心酮片, 高效液相色谱, 指纹图谱

Abstract: Objective: To construct the HPLC fingerprint of Yixintong tablets. Methods: The HPLC was used to construct the fingerprint of 13 batches Yixintong tablets with the condition that the column was ZORBAX SB-C₁₈ column (5µm, 4.6mm×250mm); the mobile phase was eluted with gradient by 0.1% formic acid-water, acetonitrile and tetrahydrofuran; the flow rate was 1.0ml/min; the detection wavelength was 350nm; the column temperature was 30℃; the sample volume was 10μl. The similarity evaluation software (2004A version) was used for similarity analysis. Results: The HPLC fingerprints of 13 batches Yixintong tablets calibrated 10 common peaks, and the similarity were all higher than 0.99. Conclusions: The method is simple, stable and feasible, and can be used to evaluate the quality of Yixintong tablets.

Key words: Yixintong tablets, HPLC, Fingerprint

中图分类号:

R917

徐宝欣, 杜义龙, 潘海峰, 李艳荣. 益心酮片HPLC指纹图谱的构建^*[J]. 承德医学院学报, 2018, 35(1): 6-8.

XU Bao-xin, DU Yi-long, PAN Hai-feng, et al. CONSTRUCTION OF HPLC FINGERPRINT OF YIXINTONG TABLETS[J]. Journal of Chengde Medical University, 2018, 35(1): 6-8.

[1]	王雪芙, 孙国祥. 甘草干姜汤的质量研究进展[J]. 承德医学院学报, 2021, 38(2): 145-148.
[2]	孙冰冰, 李莹莹, 常金花, 李松涛, 赵红玲, 王汝兴. 蛇床子素壳寡糖纳米胶束中蛇床子素的含量测定^*[J]. 承德医学院学报, 2019, 36(5): 361-364.
[3]	毕彤, 董海荣, 朱磊. HPLC法测定黄芩苷化学原料药中主成分的含量及有关物质[J]. 承德医学院学报, 2019, 36(3): 192-195.
[4]	高婧, 李明臣, 徐宝欣, 李艳荣, 潘海峰. 山楂叶四种不同提取部位指纹图谱和总黄酮含量的比较研究^*[J]. 承德医学院学报, 2018, 35(1): 1-3.