慢性脑缺血对大鼠海马钙通道Cav1.3表达的影响

承德医学院学报 ›› 2017, Vol. 34 ›› Issue (1): 5-8.

慢性脑缺血对大鼠海马钙通道Cav1.3表达的影响

郭森¹, 朱江²

1.承德医学院,河北承德 067000;
2.承德医学院附属医院

收稿日期:2016-08-01 出版日期:2017-02-10 发布日期:2021-11-24

EFFECTS OF CHRONIC CEREBRAL ISCHEMIA ON CALCIUM CHANNEL CAV1.3 EXPRESSION IN HIPPOCAMPUS OF RATS

GUO Sen, ZHU Jiang

Chengde Medical College, Hebei Cheng 067000, China

Received:2016-08-01 Online:2017-02-10 Published:2021-11-24

摘要/Abstract

摘要： 目的: 探讨慢性脑缺血后大鼠海马钙通道Cav1.3蛋白表达的变化。方法: 雄性Wistar大鼠36只随机分为假手术组、缺血7d组和缺血14d组。通过阻断左侧椎动脉和双侧颈总动脉的方法制作慢性脑缺血模型。采用尼氏染色法观察海马CA1区神经元形态结构的变化,蛋白质印迹法检测大鼠海马钙通道Cav1.3蛋白的表达情况。结果:与缺血7d组比较,缺血14d组大鼠海马CA1区神经元的病理变化明显加重。随着缺血时间的延长,海马钙通道Cav1.3蛋白的表达明显降低。结论: 慢性脑缺血可能通过降低海马钙通道Cav1.3蛋白的表达影响海马神经元的正常功能。

关键词: 慢性脑缺血, 海马, L型钙通道

Abstract: Objective: To investigate the changes of calcium channel Cav1.3 protein expression in hippocampus of chronic cerebral ischemia rats.Methods: 36 male Wistar rats were randomly divided into sham-operation group, ischemia 7 days group and ischemia 14 days group. The chronic cerebral ischemia rats’ model was established by blocking left vertebral artery and common carotid artery on both sides. Nissl’s staining was used to observe the morphology of neurons in hippocampus CA1 region,Western Blot to detect calcium channel Cav1.3 protein expression in hippocampus. Results: Compared with 7 days group, the pathological changes of neurons in hippocampus CA1 region of rats in 14 days group markedly aggravated. With prolongation of ischemic time, the calcium channel Cav1.3 protein expression obviously decreased. Conclusions: Chronic cerebral ischemia may affect the normal function of hippocampal neurons by decreasing the expression of calcium channel Cav1.3 protein in hippocampus.

Key words: Chronic cerebral ischemia, Hippocampus, L-type calcium channel

中图分类号:

R322.81

郭森, 朱江. 慢性脑缺血对大鼠海马钙通道Cav1.3表达的影响[J]. 承德医学院学报, 2017, 34(1): 5-8.

GUO Sen, ZHU Jiang. EFFECTS OF CHRONIC CEREBRAL ISCHEMIA ON CALCIUM CHANNEL CAV1.3 EXPRESSION IN HIPPOCAMPUS OF RATS[J]. Journal of Chengde Medical University, 2017, 34(1): 5-8.

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