氨茶碱对SD大鼠脂肪间充质干细胞增殖能力的影响

承德医学院学报 ›› 2017, Vol. 34 ›› Issue (3): 183-185.

氨茶碱对SD大鼠脂肪间充质干细胞增殖能力的影响

张洋洋, 杨林瀛, 庞桂芬^△, 刘娜, 姜锋

承德医学院附属医院,河北承德 067000

收稿日期:2016-09-13 出版日期:2017-06-10 发布日期:2021-11-26
通讯作者: ^△

EFFECT OF AMINOPHYLLINE ON PROLIFERATION OF ADIPOSE TISSUE-DERIVED STEM CELLS OF SD RATS

<a href="http://tougao.cdmc.edu.cn:8088/EN/article/advancedSearchResult.do?searchSQL=(((ZHANG Yang-yang[Author]) AND 1[Journal]) AND year[Order])" target="_blank">ZHANG Yang-yang</a>,<a href="http://tougao.cdmc.edu.cn:8088/EN/article/advancedSearchResult.do?searchSQL=((( YANG Lin-ying[Author]) AND 1[Journal]) AND year[Order])" target="_blank"> YANG Lin-ying</a>,<a href="http://tougao.cdmc.edu.cn:8088/EN/article/advancedSearchResult.do?searchSQL=((( PANG Gui-fen[Author]) AND 1[Journal]) AND year[Order])" target="_blank"> PANG Gui-fen</a>,<a href="http://tougao.cdmc.edu.cn:8088/EN/article/advancedSearchResult.do?searchSQL=((( et al[Author]) AND 1[Journal]) AND year[Order])" target="_blank"> et al</a>

the Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China

Received:2016-09-13 Online:2017-06-10 Published:2021-11-26

摘要/Abstract

摘要： 目的: 探讨氨茶碱对SD大鼠脂肪间充质干细胞（ASCs）增殖能力的影响。方法: 分离培养SD大鼠ASCs,取第3代ASCs,不同浓度氨茶碱（0μg/ml、5μg/ml、10μg/ml、20μg/ml）作用ASCs 48h,台盼蓝染色检测各组细胞的存活率,MTT法绘制细胞生长曲线。结果: 不同浓度氨茶碱组ASCs存活率比较,差异无统计学意义（P>0.05）;相同培养时间、不同浓度氨茶碱组ASCs增殖能力比较,差异无统计学意义（P>0.05）。结论: 在体外培养条件下,治疗浓度的氨茶碱对ASCs的存活率和增殖无明显影响。

关键词: 氨茶碱, 脂肪间充质干细胞, 细胞增殖, 细胞培养

Abstract: Objective: To explore the effects of aminophylline on proliferation of adipose tissue-derived stem cells (ASCs) of SD rats. Methods: ASCs of SD rats were isolated and cultured to the third generation. Trypan blue staining was used to detect the survival rate of ASCs, MTT methods to draw growth curve after the ASCs were cultured with aminophylline of different concentration (0μg/ml, 5μg/ml, 10μg/ml, 20μg/ml) for 48h. Results: There was no significant difference about survival rate of ASCs in different concentration aminophylline group (P>0.05). There was no significant difference about proliferation of ASCs at same culture time and of different concentration of aminophylline (P>0.05). Conclusions: Treatment concentration of aminophylline has no obvious effects on survival rate and proliferation of ASCs in vitro.

Key words: Aminophylline, Adipose tissue-derived stem cells, Proliferation, Cell culture

中图分类号:

R974

张洋洋, 杨林瀛, 庞桂芬, 刘娜, 姜锋. 氨茶碱对SD大鼠脂肪间充质干细胞增殖能力的影响[J]. 承德医学院学报, 2017, 34(3): 183-185.

ZHANG Yang-yang, YANG Lin-ying, PANG Gui-fen, et al. EFFECT OF AMINOPHYLLINE ON PROLIFERATION OF ADIPOSE TISSUE-DERIVED STEM CELLS OF SD RATS[J]. Journal of Chengde Medical University, 2017, 34(3): 183-185.

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