灵芝多糖对小鼠黑素瘤B16F10细胞分泌VEGF的抑制作用

承德医学院学报 ›› 2017, Vol. 34 ›› Issue (4): 276-278.

灵芝多糖对小鼠黑素瘤B16F10细胞分泌VEGF的抑制作用

李红, 陆洁, 段昕所, 齐海花, 孙立新^△

承德医学院附属医院,河北承德 067000

收稿日期:2016-10-14 出版日期:2017-08-10 发布日期:2021-11-26
通讯作者: ^△

INHIBITORY EFFECTS OF GANODERMA LUCIDUM POLYSACCHARIDES ON VEGF SECRETION IN B16F10 MELANOMA CELLS

the Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China

Received:2016-10-14 Online:2017-08-10 Published:2021-11-26

摘要/Abstract

摘要： 目的: 探讨灵芝多糖对B16F10黑素瘤细胞分泌血管内皮生长因子（VEGF）的影响。方法: 用不同浓度的灵芝多糖处理B16F10黑素瘤细胞,以正常培养基代替灵芝多糖培养B16F10黑素瘤细胞为对照。分别采用Western blot法和RT-PCR法检测灵芝多糖作用48h后B16F10黑素瘤细胞VEGF蛋白和mRNA表达的变化。结果: 浓度为0.8μg/ml、3.2μg/ml、12.8μg/ml的灵芝多糖处理B16F10细胞后,B16F10细胞VEGF蛋白和mRNA的表达水平均明显降低（P<0.05）。结论: 灵芝多糖可能通过抑制B16F10黑素瘤细胞分泌VEGF起到抑制肿瘤的作用。

关键词: 灵芝多糖, B16F10黑素瘤细胞, VEGF

Abstract: Objective: To investigate the effects of Ganoderma lucidum polysaccharides (GL-PS) on secretion of vascular endothelial growth factor (VEGF) in B16F10 melanoma cells. Methods: B16F10 melanoma cells were treated with or without different concentrations of GL-PS for 48h, then the expression of VEGF protein and mRNA was respectively detected by Western-blot and RT-PCR. Results: Compared with control group, the expression of VEGF protein and mRNA in B16F10 melanoma cells decreased obviously after the B16F10 melanoma cells were treated with different concentrations of GL-PS (0.8μg/ml, 3.2μg/ml, 12.8μg/ml) for 48h (P<0.05). Conclusions: GL-PS may play a role in inhibiting tumor by inhibiting secretion VEGF in B16F10 melanoma cells.

Key words: Ganoderma lucidum polysaccharides, B16F10 melanoma cells, Vascular endothelial growth factor (VEGF)

中图分类号:

李红, 陆洁, 段昕所, 齐海花, 孙立新. 灵芝多糖对小鼠黑素瘤B16F10细胞分泌VEGF的抑制作用[J]. 承德医学院学报, 2017, 34(4): 276-278.

LI Hong, LU Jie, DUAN Xin-suo, et al. INHIBITORY EFFECTS OF GANODERMA LUCIDUM POLYSACCHARIDES ON VEGF SECRETION IN B16F10 MELANOMA CELLS[J]. Journal of Chengde Medical University, 2017, 34(4): 276-278.

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