EXPRESSION AND CLINICAL SIGNIFICANCE OF SIX1 AND E-CAD IN COLON CANCER

Abstract

Abstract: Objective: To investigate the expression and clinical significance of Six1 and E-cad in colon cancer.Methods: SP immunohistochemical method was used to detect Six1 and E-cad expression in 70 cases of colon cancer and 30 cases of adjacent normal tissue; and the correlations between Six1, E-cad and the clinical pathological characteristics of colon cancer were also analyzed. Results: The positive expression rate of Six1 in colon cancer was obviously higher than adjacent normal tissue, but the positive expression rate of E-cad was obviously lower (P<0.01). The Six1 and E-cad expression in colon cancer was not related to sex, age, size of tumour and differentiation degree (P＞0.05); But related to TNM stage and infiltration depth (P＜0.05). The expression of Six1 in colon cancer was negative correlated to E-cad expression (r=-0.480, P＜0.01). Conclusions: E-cad and Six1 maybe involved in development of colon cancer together; Moreover, E-cad and Six1 expression is closely related to poor prognosis of colon cancer.

Key words: Six1, E-cad, Colon cancer

摘要： 目的: 探讨结肠癌组织同源盒蛋白1（Six1）和E-钙黏蛋白（E-cad）的表达和临床意义。方法: 采用SP免疫组化法检测70例结肠癌组织和30例癌旁正常组织Six1、E-cad的表达情况,并分析二者与结肠癌临床病理特征的关系及结肠癌组织Six1、E-cad表达的相关性。结果:结肠癌组织Six1的阳性表达率明显高于癌旁正常组织（P<0.01）,E-cad的阳性表达率明显低于癌旁正常组织（P<0.01）。结肠癌组织Six1、E-cad表达与性别、年龄、肿瘤大小、分化程度无关（P>0.05）;与TNM分期和浸润深度有关（P<0.05）。结肠癌组织Six1、E-cad表达呈负相关关系（r=-0.480,P<0.01）。结论: Six1、E-cad可能共同参与了结肠癌的发生发展过程,且二者的表达情况与结肠癌患者的不良预后密切相关。

关键词: Six1, E-cad, 结肠癌

CLC Number:

R735.3

WANG Jing-miao, JIA Xi-hua. EXPRESSION AND CLINICAL SIGNIFICANCE OF SIX1 AND E-CAD IN COLON CANCER[J]. Journal of Chengde Medical University, 2017, 34(1): 3-3.

王静苗, 贾喜花. Six1、E-cad在结肠癌中的表达及意义[J]. 承德医学院学报, 2017, 34(1): 3-3.

References

[1] 杨倩,马翔,李华驰,等.基因组学与蛋白质组学在结直肠癌研究中的进展[J].中国临床医师杂志(电子版),2013,7(3): 1252-1255.
[2] Yap AS,Crampton MS,Hardin J.Making and breaking contacts:the cellular biology of cadherin regulation[J].Curr Opin Cell Boil,2007,19(5):508-514.
[3] Yu YY,Pan YS,Zhu ZG.Homeobox genes and their functions on development and neoplasm in gastrointestinal tract[J].Eur J Surg Oncol,2007,33(2):129-132.
[4] Wheelock MJ,Johnson KR.Cadherins as modulators of cellular phenotype[J]. Annu Rev Cell Dev Biol,2003,19:207-235.
[5] David JM,Rajasekaran AK.Dishonorable discharge:the oncogenic roles of cleaved E-cadherin fragments[J].Cancer Res,2012,72(12):
2917-2923.
[6] Lim SH,Becker TM,Chua W,et al.Circulating tumour cells and the epithelial mesenchymal transition in colorectal cancer[J].J Clin Pathol,2014,67(10):848-853.
[7] Yan W,Fu Y,Tian D,et al.PI3 kinase/Akt signaling mediates epithelial-mesenchymal transition in hypoxic hepatocellular carcinoma cells[J].Biochem Biophys Res Commun,2009,382(3):631-636.
[8] Liu Y,Chu A,Chakroun I,et a1.Cooperation between myogenic regulatory factors and SIX family transcription factors is important for myoblast differentiation[J].Nucleic Acids Res,2010, 38(20):6857-6871.
[9] Wang CA,Jedlicka P,Patrick AN,et al.SIX1 induces lym phangiogenesis and metastasis via upregulation of VEGF-C in mouse models of breast cancer[J].J Clin Invest,2012,122(5):1895-1906.
[10] Tan J,Zhang C,Qian J.Expression and significance of Six1 and Ezrin in cervical cancer tissue[J].Tumour Biol,2011,32(6):1241-1247.
[11] Ono H,Imoto I,Kozaki K,et al.SIX1 promotes epithelial-mesenchymal transition in colorectal cancer through ZEB1 activation[J].Oncogene,2012,31(47):4923-4934.
[12] Schmalohfer O,Brableiz S,Brabletz T.E-cadherin, beta-catenin, and ZEB1 in malignant progression of cancer[J].Cancer Metastasis Rev,2009,28(1-2):15l-166.
[13] 赵俊卿,李云峰,杨之斌.肿瘤细胞发生细胞上皮-间质转变机制的研究[J].肿瘤,2010,30(10):890-893.