INHIBITORY EFFECTS OF GANODERMA LUCIDUM POLYSACCHARIDES ON VEGF SECRETION IN B16F10 MELANOMA CELLS

Journal of Chengde Medical University ›› 2017, Vol. 34 ›› Issue (4): 276-278.

INHIBITORY EFFECTS OF GANODERMA LUCIDUM POLYSACCHARIDES ON VEGF SECRETION IN B16F10 MELANOMA CELLS

the Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China

Received:2016-10-14 Online:2017-08-10 Published:2021-11-26

灵芝多糖对小鼠黑素瘤B16F10细胞分泌VEGF的抑制作用

李红, 陆洁, 段昕所, 齐海花, 孙立新^△

承德医学院附属医院,河北承德 067000

通讯作者: ^△

Abstract

Abstract: Objective: To investigate the effects of Ganoderma lucidum polysaccharides (GL-PS) on secretion of vascular endothelial growth factor (VEGF) in B16F10 melanoma cells. Methods: B16F10 melanoma cells were treated with or without different concentrations of GL-PS for 48h, then the expression of VEGF protein and mRNA was respectively detected by Western-blot and RT-PCR. Results: Compared with control group, the expression of VEGF protein and mRNA in B16F10 melanoma cells decreased obviously after the B16F10 melanoma cells were treated with different concentrations of GL-PS (0.8μg/ml, 3.2μg/ml, 12.8μg/ml) for 48h (P<0.05). Conclusions: GL-PS may play a role in inhibiting tumor by inhibiting secretion VEGF in B16F10 melanoma cells.

Key words: Ganoderma lucidum polysaccharides, B16F10 melanoma cells, Vascular endothelial growth factor (VEGF)

摘要： 目的: 探讨灵芝多糖对B16F10黑素瘤细胞分泌血管内皮生长因子（VEGF）的影响。方法: 用不同浓度的灵芝多糖处理B16F10黑素瘤细胞,以正常培养基代替灵芝多糖培养B16F10黑素瘤细胞为对照。分别采用Western blot法和RT-PCR法检测灵芝多糖作用48h后B16F10黑素瘤细胞VEGF蛋白和mRNA表达的变化。结果: 浓度为0.8μg/ml、3.2μg/ml、12.8μg/ml的灵芝多糖处理B16F10细胞后,B16F10细胞VEGF蛋白和mRNA的表达水平均明显降低（P<0.05）。结论: 灵芝多糖可能通过抑制B16F10黑素瘤细胞分泌VEGF起到抑制肿瘤的作用。

关键词: 灵芝多糖, B16F10黑素瘤细胞, VEGF

CLC Number:

LI Hong, LU Jie, DUAN Xin-suo, et al. INHIBITORY EFFECTS OF GANODERMA LUCIDUM POLYSACCHARIDES ON VEGF SECRETION IN B16F10 MELANOMA CELLS[J]. Journal of Chengde Medical University, 2017, 34(4): 276-278.

李红, 陆洁, 段昕所, 齐海花, 孙立新. 灵芝多糖对小鼠黑素瘤B16F10细胞分泌VEGF的抑制作用[J]. 承德医学院学报, 2017, 34(4): 276-278.

References

[1] Liu W,Xu J,Wang M,et al.Tumor-derived vascular endothelial growth factor (VEGF)-a facilitates tumor metastasis through the VEGF-VEGFR1 signaling pathway[J].Int J Oncol,2011,39(5): 1213-1220.
[2] Kim HN,Kim H,Kong JM,et al.Vitamin C down-regulates VEGF production in B16F10 murine melanoma cells via the suppression of p42/44 MAPK activation[J].J Cell Biochem,2011,112(3):894-901.
[3] Nagengast WB,Hooge MN,van Straten EM,et al.VEGF-SPECT with ¹¹¹In-bevacizumab in stage III/IV melanoma patients[J].Eur J Cancer,2011,47(10):1595-1602.
[4] 王建坤,段昕所,孙立新,等.灵芝多糖对B16F10黑素瘤细胞分泌TGF-β1的影响[J].中国皮肤性病学杂志,2014,28(11):1101-1103.
[5] 郭鹏荣,盛玉文,刘奔,等.灵芝多糖对顺铂抑制荷膀胱癌T24细胞裸鼠肿瘤生长及血管生成作用的影响[J].解放军医学杂志,2014,39(6):470-474.
[6] Khosrotehrani K,Nguyen Huu S,Prignon A,et al.Pregnancy promotes melanoma metastasis through enhanced lymphangiogenesis[J].Am J Pathol,2011,178(4):1870-1880.
[7] 李晓冰,赵宏艳,郭栋.灵芝多糖药理学研究进展[J].中成药,2012,34(2):332-334.
[8] 张晓春,陈赣玲,马兵,等.灵芝多糖抑制鸡胚尿囊膜模型中的血管生成及细胞黏附[J].基础医学与临床,2005,25(9):825-828.
[9] 梁曾恩妮,易有金,郭雨桐,等.灵芝多糖联合5-氟尿嘧啶对LoVo细胞增殖及凋亡的影响[J].中成药,2012,34(11):2068-2072.
[10] 王斌,刘瑶,邹飞,等.TRPC通道在缺氧胶质瘤细胞VEGF表达中的作用[J].中华神经医学杂志,2013,12(2):109-113.
[11] 赵建龙,李世朋,丁雪贞,等.VEGF、LYVE-1在肝癌组织中的表达与意义[J].中国老年学杂志,2014,34(4):2167-2169.
[12] 张金玲,王玥,张丽娟,等.VEGF促进人黑素瘤SK-MEL-1细胞侵袭性的自分泌机制初探[J].中国皮肤性病学杂志,2012,26(3):192-195
[13] 李建文,柯以铨.微环境对胶质瘤血管生成拟态作用的研究[J].中华神经医学杂志,2013,12(2):210-212.
[14] 殷芳,宋宁静,王晓楠,等.STAT3和VEGF在皮肤黑素瘤中的作用及研究进展[J].国际皮肤性病学杂志,2013,39(1):28-31.