Abstract: Objective: To optimize the extraction process of Salvia miltiorrhiza and Panax notoginseng in Compound Danshen preparation. Methods: Four factors and three levels L₉(3⁴) orthogonal test was used to optimize the co-extracting technology of Salvia miltiorrhiza and Panax notoginseng by taking Danshensu, tanshinone A and notoginsenoside R₁ as index component. Results: The optimal co-extracting technology of Salvia miltiorrhiza and Panax notoginseng was: 8 times of 70% ethanol ultrasonic extraction of 3 times, each time 60min, merged filtrate and filtering; the residue was ultrasonically extracted 2 times with 10 times of water, each time 30min; merged the water solution, alcohol precipitation to the alcohol content of 50% and filtering; merged the filtrate and alcohol extract, recycled ethanol and froze drying. Conclusions: The co-extracting technology of Salvia miltiorrhiza and Panax notoginseng in Compound Danshen preparation in this study is stable and simple, and the extraction efficiency is fairly high.

Key words: Compound Danshen preparation, Salvia miltiorrhiza, Panax notoginseng, Co-extraction technology, Orthogonal test

摘要： 优选复方丹参制剂中丹参、三七合提工艺。方法: 以丹参素、丹参酮ⅡA、三七皂苷R₁为指标成分,采用四因素三水平L₉(3⁴)的正交试验优选丹参与三七合提的工艺。结果: 丹参、三七合提最佳工艺为8倍量70%乙醇超声提取3次,每次60min,合并滤液,抽滤;滤渣加10倍量水超声提取2次,每次30min,合并水液,醇沉至含醇量50%,抽滤;滤液与醇提液合并,回收乙醇,冷冻干燥。结论: 本研究确定的复方丹参制剂中丹参、三七的提取工艺稳定简便,丹参和三七中有效成分的提取率均较高。

关键词: 复方丹参制剂, 丹参, 三七, 合提工艺, 正交试验