Abstract: Objective: To construct the HPLC fingerprint of Yixintong tablets. Methods: The HPLC was used to construct the fingerprint of 13 batches Yixintong tablets with the condition that the column was ZORBAX SB-C₁₈ column (5µm, 4.6mm×250mm); the mobile phase was eluted with gradient by 0.1% formic acid-water, acetonitrile and tetrahydrofuran; the flow rate was 1.0ml/min; the detection wavelength was 350nm; the column temperature was 30℃; the sample volume was 10μl. The similarity evaluation software (2004A version) was used for similarity analysis. Results: The HPLC fingerprints of 13 batches Yixintong tablets calibrated 10 common peaks, and the similarity were all higher than 0.99. Conclusions: The method is simple, stable and feasible, and can be used to evaluate the quality of Yixintong tablets.

Key words: Yixintong tablets, HPLC, Fingerprint

摘要： 目的: 构建益心酮片的HPLC指纹图谱。方法: 采用HPLC法建立13批益心酮片的指纹图谱。色谱条件：ZORBAX SB-C₁₈色谱柱（5µm,4.6mm×250mm）,流动相为A：0.1%甲酸-水混合溶液、B：乙腈、C：四氢呋喃,梯度洗脱,流速为1.0ml/min,检测波长350nm,柱温30℃,进样量10µl。使用中药指纹图谱相似度评价软件（版本2004 A）进行相似度分析。结果: 根据13批益心酮片的指纹图谱检测结果,共标定出10个共有峰,13批益心酮片的相似度均大于0.99。结论: 该方法操作简单、可靠,重复性强,结果比较稳定,适用于益心酮片的质量评价。

关键词: 益心酮片, 高效液相色谱, 指纹图谱