EFFECTS OF DIHYDROMYRICETIN ON MMP-2, PI3K, AKT EXPRESSION IN CHORIOCARCINOMA JAR CELLS

Journal of Chengde Medical University ›› 2018, Vol. 35 ›› Issue (3): 181-183.

EFFECTS OF DIHYDROMYRICETIN ON MMP-2, PI3K, AKT EXPRESSION IN CHORIOCARCINOMA JAR CELLS

Institute of Oncology, Chengde Medical College, Hebei Chengde 067000, China

Received:2017-11-27 Online:2018-06-10 Published:2021-11-21

二氢杨梅素对绒癌JAR细胞MMP-2、PI3K和Akt表达的影响^*

雷贇涛, 刘镭, 许倩, 李玉红^△, 崔莹

承德医学院肿瘤研究所,河北承德 067000

通讯作者: △
基金资助:
* 河北省高校重点学科建设项目（冀教高【2013】4号）; 河北省高校百名优秀创新人才支持计划Ⅲ(SLR2017018); 河北省教育厅项目(QN2016012)

Abstract

Abstract: Objective: To investigate the effects of dihydromyricetin on matrix metalloproteinase 2 (MMP-2), phosphatidylinositol3-kinase (PI3K) and protein kinase B (Akt) mRNA expression in choriocarcinoma JAR cells. Methods: Real time fluorescence quantitative PCR was used to detect the MMP-2, PI3K and Akt mRNA expression in JAR cells after the JAR cells were treated with dihydromyricetin (60μg/ml) for 36h; as well as the correlations between MMP-2 mRNA and PI3K, Akt mRNA in JAR cells were analyzed. Results: After the JAR cells were treated with dihydromyricetin (60μg/ml) for 36h, the MMP-2, PI3K and Akt mRNA expression in JAR cells significantly decreased compared with control group (P<0.05). Moreover, the MMP-2 mRNA expression in JAR cells was positively correlated with PI3K and Akt mRNA expression (r=0.957, 0.953, P<0.05). Conclusions: Dihydromyricetin may influence the invasion and metastasis of JAR cells by down regulating the MMP-2 gene expression in JAR cells, the mechanisms maybe related to the effects of dihydromyricetin on PI3K/Akt signaling pathway.

Key words: Dihydromyricetin, Choriocarcinoma, MMP-2, PI3K, Akt

摘要： 目的: 研究二氢杨梅素（dihydromyricetin,DMY）对绒癌JAR细胞基质金属蛋白酶2（matrix metalloproteinase 2,MMP-2）mRNA、细胞磷脂酰肌醇3-激酶（phosphatidylinositol3-kinase,PI3K）mRNA和蛋白激酶B（protein kinase B,Akt）mRNA表达的影响。方法: 采用实时荧光定量PCR法检测浓度为60μg/ml的二氢杨梅素作用36h,绒癌JAR细胞MMP-2 mRNA、PI3K mRNA、Akt mRNA表达的变化,并分析JAR细胞MMP-2 mRNA表达水平与PI3K mRNA、Akt mRNA表达水平的相关性。结果: 与对照组相比,浓度为60μg/ml的二氢杨梅素作用36h,JAR细胞MMP-2 mRNA、PI3K mRNA、Akt mRNA的表达水平明显降低（P<0.05）;并且,JAR细胞MMP-2 mRNA表达水平与PI3K mRNA、Akt mRNA表达水平呈正相关关系（r=0.957、0.953,P<0.05）。结论: 二氢杨梅素可能通过下调绒癌JAR细胞MMP-2基因的表达影响JAR细胞的侵袭和转移,其机制可能与影响PI3K/Akt信号通路有关。

关键词: 二氢杨梅素, 绒癌, MMP-2, PI3K, Akt

CLC Number:

R737.33

LEI Yun-tao, LIU Lei, XU Qian, et al. EFFECTS OF DIHYDROMYRICETIN ON MMP-2, PI3K, AKT EXPRESSION IN CHORIOCARCINOMA JAR CELLS[J]. Journal of Chengde Medical University, 2018, 35(3): 181-183.

雷贇涛, 刘镭, 许倩, 李玉红, 崔莹. 二氢杨梅素对绒癌JAR细胞MMP-2、PI3K和Akt表达的影响^*[J]. 承德医学院学报, 2018, 35(3): 181-183.

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