EFFECTS OF PICROPODOPHYLLIN ON PROLIFERATION OF HUMAN COLORECTAL CANCER HCT-15 CELLS

Journal of Chengde Medical University ›› 2018, Vol. 35 ›› Issue (6): 457-460.

EFFECTS OF PICROPODOPHYLLIN ON PROLIFERATION OF HUMAN COLORECTAL CANCER HCT-15 CELLS

Department of Pathology, the Affiliated Hospital of Chengde Medical College, Hebei Chengde 067000, China

Received:2018-06-03 Online:2018-12-10 Published:2021-11-21

鬼臼苦素对人结直肠癌HCT-15细胞增殖的影响

周丽丽¹, 刘赛璇¹, 赵杨¹, 刘莎莎¹, 王瑞婷², 申兴斌^1,△

1.承德医学院附属医院病理科,河北承德 067000;
2.承德医学院

通讯作者: △

Abstract

Abstract: Objective: To study the effects of picropodophyllin on proliferation of human colorectal cancer HCT-15 cells. Methods: Human colorectal cancer HCT-15 cells were cultured in vitro. The proliferative activity of HCT-15 cells after treating with different concentrations of picropodophyllin for 24h, 48h and 72h were respectively detected by CCK-8 assay, and the morphological changes of HCT-15 cells were observed under inverted microscope. Results: CCK8 results showed that picropodophyllin could significantly inhibit the proliferation of HCT-15 cells. In the range of 0.125μmol/L~2μmol/L, the inhibition rate of HCT- 15 cells gradually increased with the increasing of picropodophyllin concentrations and action time, in a dose-time dependent manner. Under inverted microscope, the HCT-15 cells appeared shrikage, refractive reduction and decreasing of living cells at different degree after picropodophyllin treatment. Conclusions: Picropodophyllin has significant inhibitory effects on human colorectal cancer HCT-15 cells.

Key words: Picropodophyllin, Human colorectal cancer HCT-15 cells, Proliferation

摘要： 目的: 研究鬼臼苦素对人结直肠癌细胞HCT-15增殖的影响。方法: 体外培养人结直肠癌HCT-15细胞,采用CCK-8法检测不同浓度鬼臼苦素作用HCT-15细胞24h、48h、72h后的增殖活性,并在倒置显微镜下观察HCT-15细胞形态的变化。结果: CCK8法结果显示鬼臼苦素能显著抑制HCT-15细胞的增殖活性,鬼臼苦素浓度在0.125μmol/L~2μmol/L范围内,随着鬼臼苦素浓度的增加和作用时间的延长,HCT-15细胞的抑制率逐渐增加,呈剂量-时间依赖性。倒置显微镜下观察发现,鬼臼苦素作用后HCT-15细胞不同程度皱缩、折光性降低、活细胞数明显减少。结论: 鬼臼苦素对人结直肠癌HCT-15细胞具有明显的抑制作用。

关键词: 鬼臼苦素, 人结直肠癌HCT-15细胞, 增殖

CLC Number:

R735.3

ZHOU Li-li, LIU Sai-xuan, ZHAO Yang, et al. EFFECTS OF PICROPODOPHYLLIN ON PROLIFERATION OF HUMAN COLORECTAL CANCER HCT-15 CELLS[J]. Journal of Chengde Medical University, 2018, 35(6): 457-460.

周丽丽, 刘赛璇, 赵杨, 刘莎莎, 王瑞婷, 申兴斌. 鬼臼苦素对人结直肠癌HCT-15细胞增殖的影响[J]. 承德医学院学报, 2018, 35(6): 457-460.

References

[1] Wan J, Li XM, Shu SR, et al.Effects of lentivirus-mediated siRNA targeting IGF-IR on migration and invasion abilities of endometrial carcinoma cells[J]. Chin J Pathophysiol, 2012, 28(8): 1352-1357.
[2] Gimita A, Girnita L, DelPrete F, et al. Cyelolignans as inhibitors of the insulin-like growth factor-I receptor and malignant cell growth[J]. Cancer Res, 2004, 64(1): 236-242.
[3] Stromberg T, Ekman S, Girnita L, et al.IGF-I receptor tyrosine kinase inhibition by the cyclolignan PPP induces G2/M-phase accumulation and apoptosis in multiple myeloma cells[J]. Blood, 2006, 107(2): 669-678.
[4] Yin S, Girnita A, Stromberg T, et al.Targeting the insulin-like growth factor-I receptor by picropodophyllin as a treatment option for glioblastoma[J]. Neuro Oncol, 2010, 12(1): 19-27.
[5] Girnita A, All-Ericsson C, Economou MA, et al.The insulin-like growth factor-I receptor inhibitor picropodophyllin causes tumor regression and attenuates mechanisms involved in invasion of uveal melanoma cells[J]. Clin Cancer Res, 2006, 12(4): 1383-1391.
[6] Ullrich A, Gray A, Tam AW, et al.Insulin-like growth factor-I receptor primary structure: comparison with insulin receptor suggests structural determinants that define functional specificity[J]. EMBO J, 1986, 5(10) : 2503-2512.
[7] Navarro M, Baserga R.Limited redundancy of survival signals from the type I insulin-like growth factor receptor[J]. Endocrinology, 2001, 142(3) : 1073-1081.
[8] 刘晓倩,孙立园,刘莎莎,等.大肠癌组织IGF-I、IGF-IR、GLUT-4的表达及意义[J].承德医学院学报,2018,35(1):8-11.
[9] 刘长军,侯嵩生.抗癌活性物质鬼臼类木脂素的研究进展[J].天然产物研究与开发,1997,9(3):81-89.
[10] Ekman S, Frodin JE, Harmenberg J, et al.Clinical Phase I study with an insulin-like growth factor-I receptor inhibitor: experiences in patients with squamous non-small cell lung carcinoma[J]. Acta Oncol, 2011, 50(3): 441-447.
[11] Vasileanu D, Weng WH, Gimita A, et al.The insulin-like growth factor I receptor inhibitor PPP produces only very limited resistance in tumor cells exposed to long-term selection[J]. Oncogene, 2006, 25(22): 3186-3195.