CHANGES OF DRD1 EXPRESSION IN SPINAL CORD AFTER SPINAL CORD INJURY AT SACRAL 2 SEGMENT OF RATS

Journal of Chengde Medical University ›› 2019, Vol. 36 ›› Issue (2): 91-94.

CHANGES OF DRD1 EXPRESSION IN SPINAL CORD AFTER SPINAL CORD INJURY AT SACRAL 2 SEGMENT OF RATS

Laboratory of Spinal Injury and Rehabilitation of Chengde Medical College, Hebei Chengde 067000, China

Received:2018-12-24 Online:2019-04-10 Published:2021-11-18

大鼠脊髓骶2节段损伤后脊髓DRD1表达的变化^*

陈莹, 张向, 田焕娜, 陈萌, 吴晓光, 任立群^△

承德医学院脊髓损伤与修复研究室,河北承德 067000

通讯作者: △
基金资助:
* 国家自然科学基金资助项目（81501080）,河北省教育厅重点项目（GD2015002)

Abstract

Abstract: Objective: To study the spasticity of rat tail and the changes of dopamine receptor-1 (DRD1) expression in spinal cord after spinal cord injury (SCI). Methods: Adult male Wistar rats were randomly divided into sham operation group and SCI group. The 2^nd sacral spinal cord (S₂) of rats in SCI group were transected totally; And then the tail spasticity score of rats were evaluated 2d, 7d, 14d, 30d and 60d after operation respectively. Immunofluorescence was used to detect the DRD1 expression in gray matter of sacral and coccygeal spinal cord 60d after operation. Results: The tail spasticity score of rats in SCI group 2d, 7d, 14d, 30d and 60d after operation were respectively 0, 1.10±0.28, 1.32±0.85, 2.50±0.53 and 4.15±0.36. DRD1 expression could be observed in dorsal horn (DH), intermediate zone (IMZ) and ventral horn (VH) in gray matter of sacral and coccygeal spinal cord of rats. The DRD1 expression in DH, IMZ and VH in gray matter of spinal cord of rats in SCI group were significantly lower than rats in sham operation group (P<0.05). Conclusions: Decreased expression of DRD1 in spinal cord after SCI may be associated with development of tail spasticity.

Key words: Spinal cord injury (SCI), Spasticity, Dopamine receptor-1 (DRD1), Immunofluorescence

摘要： 目的:研究脊髓损伤（spinal cord injury,SCI）后大鼠尾部的痉挛特征和脊髓多巴胺D1受体（dopamine receptor-1,DRD1）表达的变化。方法:成年雄性Wistar大鼠随机分为假手术（Sham）组和SCI组。SCI组大鼠第2骶髓（S₂）节段全横断,分别于术后2d、7d、14d、30d、60d进行大鼠尾部痉挛评分;两组大鼠均于术后60d取骶尾段脊髓,采用免疫荧光法检测大鼠脊髓灰质DRD1的表达。结果:SCI组大鼠术后2d、7d、14d、30d、60d时尾部痉挛评分分别为0、1.10±0.28、1.32±0.85、2.50±0.53、4.15±0.36分。大鼠脊髓骶尾段灰质的背角(dorsal horn,DH)、中间带（intermediate zone,IMZ）、腹角(ventral horn,VH)均可见DRD1表达。SCI组大鼠灰质DH、IMZ、VH中DRD1表达的光密度值均明显低于Sham组大鼠,组间差异有显著性（P<0.05）。结论:SCI后脊髓DRD1表达降低可能与大鼠尾部痉挛的发生发展有关。

关键词: 脊髓损伤, 痉挛, 多巴胺D1受体, 免疫荧光染色

CLC Number:

R363.2

CHEN Ying, ZHANG Xiang, TIAN Huan-na, et al. CHANGES OF DRD1 EXPRESSION IN SPINAL CORD AFTER SPINAL CORD INJURY AT SACRAL 2 SEGMENT OF RATS[J]. Journal of Chengde Medical University, 2019, 36(2): 91-94.

陈莹, 张向, 田焕娜, 陈萌, 吴晓光, 任立群. 大鼠脊髓骶2节段损伤后脊髓DRD1表达的变化^*[J]. 承德医学院学报, 2019, 36(2): 91-94.

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