EFFECTS OF HESHOUWUYIN ON P53, BCL-2 AND BAX MRNA EXPRESSION IN AGING SPERMATOGENIC CELLS

Journal of Chengde Medical University ›› 2019, Vol. 36 ›› Issue (3): 185-189.

EFFECTS OF HESHOUWUYIN ON P53, BCL-2 AND BAX MRNA EXPRESSION IN AGING SPERMATOGENIC CELLS

Medical College of Hebei University, Hebei Baoding 071000, China

Received:2019-01-09 Online:2019-06-10 Published:2021-11-18

何首乌饮对衰老生精细胞p53、Bcl-2和Bax mRNA表达的影响^*

单博颖¹, 薛亚然¹, 梁思², 张研¹, 齐峰^3△, 牛嗣云^1△

1.河北大学医学院,河北保定 071000;
2.河北大学附属医院;
3.保定市第一医院

通讯作者: △
基金资助:
* 国家自然科学基金项目（81673714）

Abstract

Abstract: Objective: To investigate the effects of Heshouwuyin on p53, Bcl-2 and Bax mRNA expression in aging spermatogenic cells. Methods: The spermatogenic cells aging model was established by free radical oxidative damaging method, and RT-qPCR was used to detect the p53, Bcl-2 and Bax mRNA expression in spermatogenic cells in each group. Results: The p53, Bax mRNA expression and Bax/Bcl-2 of spermatogenic cells in aging group were significantly higher than those in normal group (P<0.05),while Bcl-2 mRNA expression was significantly lower (P<0.05). The p53, Bax mRNA expression and Bax/Bcl-2 of spermatogenic cells in Heshouwuyin group were significantly lower than those in aging group (P<0.05),while Bcl-2 mRNA expression was significantly higher (P<0.05). Conclusions: Heshouwuyin can inhibit apoptosis of spermatogenic cells by upregulating Bcl-2 expression and downregulating p53 and Bax expression, so it can delay aging of spermatogenic cells.

Key words: Heshouwuyin, Aging, Spermatogenic cells, p53, Bax, Bcl-2, Apoptosis

摘要： 目的:探讨何首乌饮对衰老生精细胞p53、Bcl-2、Bax mRNA表达的影响。方法:采用自由基氧化损伤法建立生精细胞衰老模型,采用实时荧光定量PCR法检测各组生精细胞p53、Bcl-2和Bax mRNA的表达情况。结果:衰老组生精细胞p53、Bax mRNA的表达水平及Bax/Bcl-2比值明显高于正常组（P<0.05）,Bcl-2 mRNA的表达水平明显低于正常组（P<0.05）;何首乌饮干预组p53、Bax mRNA的表达水平及Bax/Bcl-2比值明显低于衰老组（P<0.05）,Bcl-2 mRNA的表达水平明显高于衰老组（P<0.05）。结论:何首乌饮可通过上调衰老生精细胞Bcl-2的表达、下调p53和Bax的表达抑制生精细胞凋亡,发挥延缓生精细胞衰老的作用。

关键词: 何首乌饮, 衰老, 生精细胞, p53, Bax, Bcl-2, 凋亡

CLC Number:

R394.3

SHAN Bo-ying, XUE Ya-ran, LIANG Si, et al. EFFECTS OF HESHOUWUYIN ON P53, BCL-2 AND BAX MRNA EXPRESSION IN AGING SPERMATOGENIC CELLS[J]. Journal of Chengde Medical University, 2019, 36(3): 185-189.

单博颖, 薛亚然, 梁思, 张研, 齐峰, 牛嗣云. 何首乌饮对衰老生精细胞p53、Bcl-2和Bax mRNA表达的影响^*[J]. 承德医学院学报, 2019, 36(3): 185-189.

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