Abstract: Objective: To analyze the effects of tropomodulin1 (Tmod1) on urine concentration in kidney of mice. Methods: Tubule-specific Tmod1 knockout mice, Tmod1^flox/flox/Ksp-cre⁺ (TFK) and its control mice Tmod1^flox/flox/Ksp-cre^-(TF)were tanken as research object in this study. The urine of mice was collected and the 24-hour urine volume as well as urine osmolality of all the mice were measured at basic state, water deprivation and water loading using metabolic cage system. Results: At basic state, water deprivation and water loading, the 24-hour urine volume of TFK mice was remarkably lower than TF mice, while the urine osmolality of TFK mice was remarkably higher than TF mice (P<0.05). Conclusions: Tmod1 may be involved in the process of urine concentration in mice, but the mechanisms need to be further studied.

Key words: Tropomodulin1 (Tmod1), Kidney, Urine concentration, Mouse

摘要： 目的:分析原肌球调节蛋白1（tropomodulin1,Tmod1）对小鼠肾脏尿浓缩功能的影响。方法:采用肾脏集合管和肾小管特异性敲除Tmod1小鼠Tmod1^flox/flox/Ksp-Cre⁺（TFK）及其对照小鼠Tmod1^flox/flox/Ksp-Cre^-（TF）为研究对象,利用代谢笼系统,分别在基础状态,以及给予小鼠禁水和水负荷处理,收集小鼠尿液测量小鼠24小时尿量和渗透压。结果:基础状态,禁水和水负荷条件下,TFK小鼠的尿量均明显少于TF小鼠,尿液渗透压明显高于TF小鼠,差异具有统计学意义（P<0.05）。结论:Tmod1可能参与了小鼠尿浓缩的过程,但机制有待深入研究。

关键词: 原肌球调节蛋白1（Tmod1), 肾脏, 尿浓缩, 小鼠