EXPERIMENTAL STUDY ON BONE MARROW MESENCHYMAL STEM CELL EXOSOMES REGULATING STAT3/GAP43 PATHWAY TO PROMOTE AXONAL GROWTH OF PRIMARY SENSORY NEURONS

Journal of Chengde Medical University ›› 2020, Vol. 37 ›› Issue (1): 1-4.

EXPERIMENTAL STUDY ON BONE MARROW MESENCHYMAL STEM CELL EXOSOMES REGULATING STAT3/GAP43 PATHWAY TO PROMOTE AXONAL GROWTH OF PRIMARY SENSORY NEURONS

Chengde Medical College, Hebei Chengde 067000, China

Received:2019-08-28 Online:2020-02-10 Published:2021-11-19

BMSC外泌体调控STAT3/GAP43通路促进初级感觉神经元轴突生长的实验研究^*

王新¹, 王志杰², 李波³, 任立群, 王天仪⁴, 郭晓玲^4△

1.承德医学院,河北承德 067000;
2.承德医学院附属医院儿内科;
3.天津医科大学总医院骨科;
4.中国人民解放军联勤保障部队第九八一医院

通讯作者: △
基金资助:
* 河北省自然科学基金面上项目(H2017101030); 中央军委后勤保障部卫生局全军医学科技青年培育计划成长项目（16QNP074）; 总后卫生部全军医学科技青年培育项目（13QNP017）; 承德市科学技术研究与发展计划（201606A062,201701A125,201701A127）

Abstract

Abstract: Objective: To explore the effects of bone marrow mesenchymal stem cell (BMSC) exosomes on STAT3/GAP43 pathway and axonal growth of primary sensory neurons. Methods: BMSC of Wistar rats was extracted and the cell morphology was observed and identified by light microscope. Hypercentrifugation was used to extract BMSCs exosomes and identified the exosomes. Primary sensory neurons (rat dorsal root ganglion neurons) were treated with BMSC exosomes, immunofluorescence staining was used to detect axon growth of primary sensory neurons, and Western Blotting to detect STAT3 and GAP43 protein expression in primary sensory neurons. Results: Immunofluorescence staining showed that the extracted cells expressed CD29 and CD90 simultaneously, which proved the extracted cells were BMSC. Western Blotting showed the extracted exosomes expressed HSP70 and Alix. Compared with the control group, the length of neuronal axon in BMSC exosomes group increased significantly, the expression of STAT3 and GAP43 protein also significantly increased in BMSC exosomes group (P<0.05). Conclusions: BMSC exosomes can promote axonal growth of primary sensory neurons by regulating STAT3/GAP43 pathway.

Key words: Bone marrow mesenchymal stem cell (BMSC), Exosome, Spinal cord injury, Axon, STAT3, GAP43

摘要： 目的探索骨髓间充质干细胞（bone marrow mesenchymal stem cell,BMSC）外泌体对初级感觉神经元STAT3/GAP43通路和轴突生长的调控作用。方法提取Wistar大鼠BMSC,光学显微镜观察细胞形态并进行鉴定;使用超速离心法提取BMSC外泌体并鉴定。使用提取的BMSC外泌体处理原代培养初级感觉神经元（大鼠背根神经节神经元）,细胞免疫荧光染色检测初级感觉神经元轴突的生长情况,Western Blotting法检测初级感觉神经元STAT3和GAP43蛋白的表达情况。结果细胞免疫荧光染色显示所提取的细胞同时表达CD29和CD90,证实为BMSC;Western Blotting显示所提取的外泌体表达HSP70和Alix。相比于对照组,BMSC外泌体组神经元轴突的长度明显增加,STAT3与GAP43蛋白的表达明显升高(P<0.05)。结论 BMSC外泌体可通过调控STAT3/GAP43通路促进初级感觉神经元轴突生长。

关键词: 骨髓间充质干细胞（BMSC）, 外泌体, 脊髓损伤, 轴突, STAT3, GAP43

CLC Number:

WANG Xin, WANG Zhi-jie, LI Bo, et al. EXPERIMENTAL STUDY ON BONE MARROW MESENCHYMAL STEM CELL EXOSOMES REGULATING STAT3/GAP43 PATHWAY TO PROMOTE AXONAL GROWTH OF PRIMARY SENSORY NEURONS[J]. Journal of Chengde Medical University, 2020, 37(1): 1-4.

王新, 王志杰, 李波, 任立群, 王天仪, 郭晓玲. BMSC外泌体调控STAT3/GAP43通路促进初级感觉神经元轴突生长的实验研究^*[J]. 承德医学院学报, 2020, 37(1): 1-4.

[1]	ZHANG Mei-ling, WANG Xin, LI Bo4, WANG Zhi-jie, GUO Xiao-ling, WANG Feng-yan, XIU Yu-cai, WANG Tian-yi. BMSC Exosomes Promote Sensory Conduction Function Recovery via Stat3/Gap43 Axis Post Dorsal Column Injury [J]. Journal of Chengde Medical University, 2021, 38(2): 91-95.
[2]	LIU Ye1, LIU Bin. Mechanism of Matrine Inhibiting Human Colon Cancer Cells Through IL-6/STAT3 Signaling Pathway [J]. Journal of Chengde Medical University, 2021, 38(2): 100-102.
[3]	ZHANG Xue. Effects of Motor Imagery Therapy on Lower Limb Function Rehabilitation and Self-efficacy in Patients with Spinal Cord Injury [J]. Journal of Chengde Medical University, 2021, 38(1): 13-16.
[4]	CHEN Ying, ZHANG Xiang, TIAN Huan-na, et al. CHANGES OF DRD1 EXPRESSION IN SPINAL CORD AFTER SPINAL CORD INJURY AT SACRAL 2 SEGMENT OF RATS [J]. Journal of Chengde Medical University, 2019, 36(2): 91-94.