Abstract: Objective To investigate the effect and mechanism of Astragalus and Hirudin on Glomerular Mesangial proliferation and the levels of MMP-9 and NF-κB. Methods Twenty-five female mice were randomly divided into 5 groups, named normal group, model group, and intervention groups which areastragalus polysaccharide group, astragaloside group and hirudin group respectively; 5 mice in each group.After lipopolysaccharide induced the proliferation of mesangial cells in mice, CCK8 method was used to detect the active ingredients of the maximum non-toxic concentration of astragalus and leech. Cells were collected and flow cytometry was used to detect the apoptosis of each group after 24h; Immunocytochemistry and qPCR were employed for detecting the expression of MMP-9 and NF-κB. Results The apoptosis rate of each intervention group was higher than that of the model group and the normal group (P<0.05); Compared with the model group, the expressions of MMP-9 and NF-κB in each intervention group were significantly reduced, but were higher than that of the normal group (P<0.05). Conclusion The different active ingredients in Astragalus and Leech may reduce the expression of MMP-9 and NF-κB protein, inhibit the release of inflammatory factors and degrade the extracellular matrix, thereby protecting the renal basement membrane to inhibit mesangial cell proliferation so as to avoid kidney damage.

Key words: astragalus, hirudin, glomerular mesangial hyperplasia, MMP-9, NF-κB;

摘要： 目的 探究黄芪、水蛭中有效成分对肾小球系膜增生作用机制及对MMP-9、NF-κB水平的影响。方法 选取25只雌性小鼠,随机分为5组,分别为正常组、模型组及三个干预组—黄芪多糖组、黄芪甲苷组、水蛭素组,每组5只。脂多糖诱导小鼠系膜细胞增殖后,选用CCK8法检测最大无毒浓度的黄芪、水蛭有效成分,进行干预24h后收集细胞。采用流式细胞术观察各组细胞凋亡情况,采用免疫细胞化学与qPCR检测MMP-9及NF-κB表达情况。结果 各干预组细胞凋亡率均高于模型组与正常组（P<0.05）。与模型组相比,各干预组MMP-9和NF-κB表达均显著下降,但均高于正常组（P<0.05）。结论 黄芪及水蛭中不同有效成分可通过降低MMP-9及NF-κB蛋白表达量,抑制炎症因子释放,降解细胞外基质,从而保护肾脏基底膜,以达到抑制系膜细胞增生、治疗肾脏损伤的目的。

关键词: 黄芪, 水蛭, 肾小球系膜增生, MMP-9, NF-кB