Abstract: Objective To explore the effect of miR-708 on the cure rate and survival rate of childhood acute lymphoblastic leukemia (ALL) and the prognostic markers. Methods Blood samples of 90 children who had been diagnosed with ALL and of 20 healthy children were collected; qRT-PCR detection technology was used to detect the expression level of miR-708 in all blood samples; the unpaired t-test was used to analyze the relationship between the two groups correlation; the receiver operating characteristic(ROC) curve was used to analyze the diagnostic value of miR-708 in children with ALL; the Kaplan-Meier survival curve method was used to evaluate the prognostic survival of miR-708 expression level in children with ALL. Results Compared with blood samples from healthy children, the expression of miR-708 in children ALL blood samples was significantly up-regulated(P<0.001); the ROC curve showed that the area under the curve, that is, the AUC value was 0.882, indicating that miR-708 had a good diagnostic potential in children's ALL; Kaplan-Meier survival curve suggested that overexpression of miR-708 was associated with poor prognosis. Conclusion miR-708 is significantly up-regulated in the blood of childhood ALL patients, and can be used as an effective marker for the diagnosis and prognosis of childhood ALL.

Key words: miR-708, childhood acute lymphoblastic leukemia, diagnosis, prognosis

摘要： 目的 探讨miR-708对儿童急性淋巴细胞白血病（ALL）的治愈率和存活率的提高作用以及预后标志物。方法 采集90例已确诊为ALL的儿童血液样本和20例健康儿童血液样本;qRT-PCR检测技术检测所有血液样本中miR-708的表达水平;非配对t检验分析两组之间的相关性;采用利用接受者操作特征（ROC）曲线分析miR-708在儿童ALL患者中的诊断价值;以Kaplan-Meier生存曲线法评估miR-708表达水平对儿童ALL的预后生存情况。结果 与健康儿童血样相比,miR-708在儿童ALL血样中表达显著上调（P<0.001）;ROC曲线显示曲线下面积（AUC）为0.882,说明miR-708在儿童ALL中具有良好的诊断潜力;Kaplan-Meier生存曲线提示过表达的miR-708与患者不良预后相关。结论 miR-708在儿童ALL患者血液中显著上调,并可以作为儿童ALL的诊断及预后的有效标志物。

关键词: miR-708, 儿童急性淋巴细胞白血病, 诊断, 预后