Abstract: Objective To investigate the changes of serum Spexin level before and after standard steamed bread meal in type 2 diabetes mellitus patients. Methods Forty patients with newly diagnosed type 2 diabetes mellitus were selected for standard steamed bread meal test. Serum samples were collected before test, 60 minutes after test, 120 minutes after test and 180 minutes after test. Serum Spexin levels were determined with Elisa method. Results Fasting serum Spexin levels were negatively correlated with fasting blood glucose, glycated hemoglobin, BMI, low density lipoprotein cholesterol, uric acid and HOMA-IR（P<0.05）, and independently correlated with BMI（t=-3.354,P=0.002）, glycated hemoblobin（t=-3.477,P=0.001）, low density lipoprotein cholesterol（t=-2.062,P=0.047） and HOMA-IR（t=-1.985,P=0.045）. After standard steamed bread meal test, serum Spexin concentration gradually decreased, the concentration was lowest at 60 minutes（P<0.05）, and recovered gradually. Serum Sepxin levels in 180 minutes after test were similar to those in fasting state（P>0.05）, and the serum Spexin concentration was negatively correlated with the blood glucose level（P<0.05）. Conclusion Serum Spexin levels in type 2 diabetes mellitus were negatively correlated with blood glucose and low density lipoprotein cholesterol. After standard steamed bread meal test, serum Spexin level had gradually decreased and gradually recovered, suggesting that Spexin may be involved in regulating the pathway of glycolipid metabolism in type 2 diabetes mellitus.

Key words: type 2 diabetes mellitus, Spexin, blood glucose, low density lipoprotein cholesterol

摘要： 目的 了解2型糖尿病患者馒头餐前后血清人神经肽(Spexin)水平的变化。方法 选取40例初次诊断的2型糖尿病患者,统一进行标准馒头餐试验,于试验前、试验后60min、120min及180min分别采血离心收集血清保存,采用Elisa法测定血清Spexin水平。结果 空腹血清Spexin水平与空腹血糖、糖化血红蛋白、BMI、低密度脂蛋白胆固醇、尿酸及HOMA-IR均呈负相关（P<0.05）;与BMI（t=-3.354,P=0.002）,糖化血红蛋白（t=-3.477,P=0.001）、低密度脂蛋白胆固醇（t=-2.062,P=0.047）及HOMA-IR（t=-1.985,P=0.045）独立相关。馒头餐后患者血清Spexin浓度逐渐下降,60min浓度最低（P<0.05）,后逐渐恢复,馒头餐后180min血清Spexin浓度与空腹状态比较无统计学差异（P>0.05）;且血清Spexin浓度与血糖水平呈负相关（P<0.05）。结论 2型糖尿病患者血清Spexin水平与血糖、低密度脂蛋白胆固醇呈负相关,馒头餐试验后血清Spexin水平逐渐下降,后逐渐恢复,提示Spexin可能参与调控2型糖尿病患者糖脂代谢的通路。

关键词: 2型糖尿病, 人神经肽, 血糖, 低密度脂蛋白胆固醇