Abstract: Objective To study the mechanism of matrine inhibiting human colon cancer cells through the IL-6/STAT3 signaling pathway. MethodHuman colon cancer cell HCT-116 was interfered with matrine, cell proliferation assay CCK-8 was used to detect cell proliferation, and the protein expression level of inflammatory factor IL-6/STAT3 gene and the protein expression level of metastatic power of colon cancer cell E-CAD gene were detected by Western blot assay. Results The cell proliferation test showed that the cell proliferation of 2mg/mL, 3mg/mL and 4mg/mL matrine groups was significantly lower than that of the control group. Western blot results showed that the matrine group had IL-6 / STAT3 protein compared with the control The expression level was significantly decreased (P<0.05), and the expression level of E-Cad protein was significantly increased(P<0.05). Conclusion Matrine can reduce the inflammatory response of human colon cancer cell HCT-116 by inhibiting IL-6/STAT3 signaling pathway, and further inhibit the proliferation and invasiveness of human colon cancer cell HCT-116, so as to achieve anti-tumor effect.

Key words: HCT-116, IL-6, STAT3, matrine, colon cancer

摘要： 目的 探讨苦参碱通过IL-6/STAT3信号通路抑制人结肠癌细胞增殖、转移的机制研究。方法 通过使用苦参碱干预人结肠癌细胞HCT-116,使用细胞增殖实验CCK-8检测细胞增殖力,使用Western blot实验检测炎性因子IL-6/STAT3基因蛋白表达水平以及结肠癌细胞转移力E-Cad基因蛋白表达水平。结果 细胞增殖试验显示,2mg/mL、3mg/mL、4mg/mL苦参碱组较对照组细胞增殖力明显降低。Western blot结果显示,苦参碱组较对照组IL-6/STAT3蛋白表达量明显降低（P<0.05）,E-Cad蛋白表达量明显升高（P<0.05）。结论 苦参碱可通过抑制IL-6/STAT3信号通路降低人结肠癌细胞HCT-116炎性反应,进一步抑制人结肠癌细胞HCT-116的增殖和转移力,从而达到抗肿瘤作用。

关键词: HCT-116, IL-6, STAT3, 苦参碱, 结肠癌