Abstract: Objective To investigate the effects of annexinA7 (ANXA7) overexpression on the proliferation and apoptosis of human gastric cancer MGC-803 cells. Methods Human gastric cancer MGC-803 cells were divided into ANXA7 overexpression group and negative control group. PcDNA3.1-ANXA7 recombinant plasmid and empty plasmid were transfected into ANXA7 overexpression group and negative control group respectively by liposome transfection. The overexpression of ANXA7 was detected by Western blot. CCK-8 assay was used to detect the proliferation ability of cells in each group. Cell apoptosis rate was detected by flow cytometry, and the expressions of PCNA, CyclinD1, Casepase-3 and Cleaved Casepase-3 were detected by Western blot. Results Compared with the negative control group, the proliferation ability of MGC-803 cells in the ANXA7 overexpression group was significantly enhanced, and the expression of PCNA and CyclinD1 were both increased (P<0.05). Apoptosis rate and Cleaved casepase-3 expression were decreased (P<0.05). The expression of Casepase-3 was not statistically significant (P>0.05). Conclusion The overexpression of ANXA7 can promote the proliferation and inhibit the apoptosis of human gastric cancer MGC-803 cells.

Key words: ANXA7, MGC-803 cells, proliferation, apoptosis

摘要： 目的 探讨膜联蛋白A7（ANXA7）过表达对人胃癌MGC-803细胞增殖和凋亡的影响。方法 将人胃癌MGC-803细胞分为ANXA7过表达组、阴性对照组,脂质体转染法将pcDNA3.1-ANXA7重组质粒以及空质粒分别转染ANXA7过表达组和阴性对照组。采用Western blot鉴定ANXA7过表达;转染48h采用CCK-8实验检测各组细胞增殖能力;流式细胞术检测各组细胞凋亡率;Western blot检测各组细胞增殖和凋亡相关蛋白PCNA、CyclinD1、Casepase-3、Cleaved casepase-3的表达。结果 与阴性对照组相比,ANXA7过表达组MGC-803细胞增殖能力显著增强、PCNA与CyclinD1的表达均升高(P<0.05);细胞凋亡率降低、Cleaved casepase-3的表达降低(P<0.05);Casepase-3的表达变化无统计学意义(P>0.05)。结论 ANXA7过表达可促进人胃癌MGC-803细胞的增殖并抑制其凋亡。

关键词: ANXA7, MGC-803细胞, 增殖, 凋亡