Abstract: Objective To study the effect of production of Interleukin-10(IL-10) in the B16F10 cells by Ganoderma lucidum polysaccharides. Methods The B16F10 cell was prepared and treated with different concentrations of Ganoderma lucidum. The expression levels of IL-10 were assessed by Western-blot, RT-PCR. Results Under Ganoderma lucidum polysaccharides at different concentrations, RT-PCR revealed that the amount of IL-10 mRNA released by B16F10 melanoma cells treated with 0μg/mL, 0.2μg/mL, 0.8μg/mL, 3.2μg/mL, 12.8μg/mL was 1.03±0.15, 1.15±0.25, 0.92±0.11, 0.86±0.64 and 0.75±0.12, respectively. Compared with the 0μg/mL Ganoderma lucidum polysaccharide group, the other groups showed statistical significance except the 0.2μg/mL Ganoderma lucidum polysaccharide group, P<0.05. Western-blot revealed that the amount of IL-10 produced by B16F10 melanoma cell treated with 0μg/mL,0.2 μg/mL, 0.8μg/mL, 3.2μg/mL, 12.8μg/mL was 1.47±0.43, 1.58±0.31, 0.93±0.12, 0.89±0.26 and 0.40±0.29, respectively. Compared with the 0μg/mL Ganoderma lucidum polysaccharide group, the 12.8μg/mL Ganoderma lucidum polysaccharide group showed statistical significance, P<0.05. Conclusion The production of IL-10 in the B16F10 cells was inhibited by Ganoderma lucidum polysaccharides.

Key words: melanoma, Ganoderma lucidum polysaccharides, IL-10, RT-PCR, Western-blot

摘要： 目的 探讨灵芝多糖对B16F10黑素瘤细胞产生白介素10（IL-10）的影响。方法 分别将不同浓度灵芝多糖作用于B16F10黑素瘤细胞,通过RT-PCR方法和蛋白印迹(Western-blot)技术检测IL-10的表达情况。结果 在不同浓度灵芝多糖作用下,经RT-PCR方法检测发现,加入0μg/mL、0.2μg/mL、0.8μg/mL、3.2μg/mL、12.8μg/mL灵芝多糖的B16F10黑素瘤细胞释放IL-10的量分别为1.03±0.15、1.15±0.25、0.92±0.11、0.86±0.64、0.75±0.12,与0μg/mL灵芝多糖组相比较,除0.2μg/mL灵芝多糖组,其他组差异均具有统计学意义,P<0.05。经Western-blot法检测,加入0μg/mL、0.2μg/mL、0.8μg/mL、3.2μg/mL、12.8μg/mL灵芝多糖的B16F10黑素瘤细胞IL-10表达量分别为1.47±0.43、1.58±0.31、0.93±0.12、0.89±0.26、0.40±0.29,与0μg/mL灵芝多糖组相比较,12.8μg/mL灵芝多糖组差异有统计学意义,P<0.05。结论 灵芝多糖对B16F10黑素瘤细胞产生IL-10具有抑制作用。

关键词: B16F10黑素瘤细胞, 灵芝多糖, IL-10, RT-PCR, 蛋白印迹技术