miR-638 Targets Down-Regulation of MACC1 to Inhibit the Malignant Progression of Gastric Cancer Cells

Abstract

Abstract: Objective To explore the biological functions and specific molecular mechanisms of miR-638 in gastric cancer. Methods The mRNA expressions of miR-638 and MACC1 in gastric cancer cells were detected by QRT-PCR, and the protein expression of MACC1 in gastric cancer cells was detected by Western blot. The dual luciferase experiment verified the targeted binding relationship between MACC1 and miR-638. The CCK8 method was used to determine the cell proliferation activity. The scratch healing test and transwell test were used to evaluate its migration and invasion capabilities. Flow cytometry experiments to detect cell cycle distribution. Results miR-638 is down-regulated in gastric cancer cells. Overexpression of miR-638 inhibits the proliferation, migration and invasion of gastric cancer cells. MACC1 expression is significantly up-regulated in gastric cancer cells, miR-638 targets the inhibition of MACC1 expression. miR-638 inhibits the proliferation, migration and invasion of gastric cancer cells by targeted down-regulation of MACC1. Conclusion miR-638 inhibits the occurrence and development of gastric cancer cells by targeting down-regulation of MACC1.

Key words: miR-638, MACC1, proliferation, migration, invasion

摘要： 目的探究miR-638在胃癌中的生物学功能和具体分子机制。方法采用qRT-PCR检测miR-638和MACC1在胃癌细胞中的mRNA表达,Western blot检测MACC1在胃癌细胞中的蛋白表达;双荧光素酶实验验证MACC1和miR-638靶向结合关系;采用CCK8法测定细胞增殖活性;用划痕愈合实验和Transwell实验评价其迁移和侵袭能力;流式细胞术检测细胞周期分布情况。结果 miR-638在胃癌细胞中表达下调;过表达miR-638抑制胃癌细胞的增殖、迁移、侵袭能力;MACC1在胃癌细胞中表达显著上调,miR-638靶向抑制MACC1的表达。miR-638是通过靶向下调MACC1抑制胃癌细胞的增殖、迁移和侵袭能力。结论 miR-638通过靶向下调MACC1抑制胃癌细胞的发生发展。

关键词: miR-638, MACC1, 增殖, 迁移, 侵袭

CLC Number:

R735.2

WU Xian-lei, QIN Er-yun, MENG Yan, ZHANG Ling-qiao. miR-638 Targets Down-Regulation of MACC1 to Inhibit the Malignant Progression of Gastric Cancer Cells[J]. Journal of Chengde Medical University, 2021, 38(6): 451-457.

吴现磊, 秦二云, 孟妍, 张令巧. miR-638靶向下调MACC1抑制胃癌细胞的恶性进展[J]. 承德医学院学报, 2021, 38(6): 451-457.

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