Abstract: Objective To establish an HPLC method for measuring the content of Chaihu saponina, c and d, which is used to compare the differences in the content of wild beichaihu in different origin cultivation areas of Beichaihu and Chengde City, Hebei Province. Methods The content of Chaihu saponin a, c and d in 27 batches of different samples of Beichaihu was determined by high-efficiency liquid chromatography, and the correlationship was analysed. Results The quality of cultivated chaihu was significantly higher than that of wild Chaihu, the highest level of Chaihu saponin a was in Beichahu, Gansu Kang County, and the highest content of Chaihu saponin d was in Beichaihu of Gansu Province, most of which were not detected by the wild beihu saponin c, and the cultivation of beihu saponin c was detected. The color and saponin content of Chaihugen were significantly correlated, the yellow root saponin a, d content was the highest, the black root content was second, and the root color had little effect on the content of Chaihu saponin c. Conclusion The content of artificial cultivation of Beichaihu saponina, c and d is higher than that of wild Chaihu, and it is found that the content of beichaihugen color and saponin content are significantly correlated, which provides theoretical basis for the establishment and evaluation of Chaihu resource utilization and quality analysis methods of medicinal herbs.

Key words: bupleurum chinense, the root color, bupleurin, HPLC

摘要： 目的 建立HPLC测定柴胡皂苷a、c、d含量方法,用于比较不同产地栽培北柴胡和河北省承德地区野生北柴胡的含量差别;对不同来源北柴胡的根色与其皂苷含量进行比较分析,为柴胡入药选择及质量评价提供科学依据。方法 采用高效液相色谱法测定27批不同北柴胡样品中的柴胡皂苷a、c、d含量,以北柴胡根色为参照组分,进行相关性分析。结果 栽培柴胡质量明显高于野生柴胡,柴胡皂苷a最高的是甘肃康县的北柴胡,柴胡皂苷d含量最高的是甘肃渭源的北柴胡,其中大部分野生北柴胡没有检测出柴胡皂苷c,栽培北柴胡均检测出柴胡皂苷c;柴胡根的颜色和皂苷含量呈显著相关,黄色根皂苷a、d含量最高,黑色根含量次之,根色对柴胡皂苷c含量几乎无影响。结论 人工栽培北柴胡皂苷a、c、d含量均高于野生柴胡,同时通过相关性分析发现,北柴胡根色与皂苷含量呈显著性相关,为柴胡资源利用和药材质量分析方法的建立与评价提供理论依据。

关键词: 北柴胡, 根色, 柴胡皂苷, HPLC