Abstract: Objective To investigate the expression characteristics and clinical significance of P-selectin in benign and malignant hydatidiform mole tissues. Methods 50 cases of benign moles and 22 cases of malignant moles were selected. qRT-PCR technology is used to detect the expression level of P-selectin mRNA. Immunohistochemistry SP method and Western Blot technology comprehensively detected the expression level of P-Selectin protein. Results Compared with benign hydatidiform mole tissue, the relative expression of mRNA and protein and protein positive expression rate of P-selectin in malignant hydatidiform mole tissue were significantly higher (P<0.05). Conclusion P-selectin participates in the development of malignant hydatidiform mole at the gene and protein level, and is expected to become a diagnostic indicator for evaluating benign and malignant hydatidiform moles, providing new ideas for clinical targeted therapy.

Key words: P-selectin, hydatidform mole, differential diagnosis

摘要： 目的 探讨分析P-选择素(P- selectin)在良、恶性葡萄胎组织中的表达特点及临床意义。方法 选取50例良性葡萄胎和22例恶性葡萄胎组织,采用实时荧光定量PCR（qRT-PCR）技术检测P-选择素mRNA表达水平。采用免疫组化SP法和蛋白印迹（Western Blot）技术综合检测P-选择素蛋白表达水平。结果 与良性葡萄胎组织相比,恶性葡萄胎组织中P-选择素mRNA、蛋白相对表达量和蛋白阳性表达率显著增高（P<0.05）。结论 P-选择素在基因、蛋白水平上均参与了葡萄胎恶性的发展,有望成为评价良、恶性葡萄胎的诊断指标,为临床靶向治疗提供新思路。

关键词: P-选择素, 葡萄胎, 鉴别诊断