Expression of miR-155-5p and its Relationship with Apoptosis Factors in Glioma

Abstract

Abstract: Objective To detect the expression of miR-155-5p in glioma, and analyze its relationship with apoptosis factors. Methods Forty-seven patients who were diagnosed with glioma and underwent surgical treatment in Nanyang Second People's Hospital from January 2016 to January 2017 were selected as the research object. The expression of miR-155-5p was detected by real-time fluorescence quantitative PCR (qPCR), and the expression of Caspase-3 was detected by Western blot. The expression of Bcl-2 was detected by immunohistochemistry. Results There were significant differences in the expression of miR-155-5p in different tumor diameters and different WHO classification groups (P<0.05). High expression of miR-155-5p had short survival time by survival analysis(P<0.05). Negative correlatin was found between miR-155-5p and Caspase-3 (P<0.05). Positive correlation was found between miR-155-5p and Bcl-2 (P<0.05). Conclusion The expression of miR-155-5p is increased in gliomas, which is involved in the formation and progression of lesions. The expression of miR-155-5p is related to apoptosis. The detection of the expression of miR-155-5p has a certain value in judging the prognosis.

Key words: glioma, miR-155-5p, apoptosis, prognosis

摘要： 目的检测miR-155-5p在胶质瘤中的表达特征,探讨miR-155-5p与凋亡相关因子的关系。方法选择2016年1月~2017年1月在南阳市第二人民医院确诊为胶质瘤并进行手术治疗的47例患者作为研究对象,应用实时荧光定量PCR法（qPCR）检测miR-155-5p的表达,应用Western Blot法检测含半胱氨酸的天冬氨酸蛋白水解酶-3（Caspase-3）的表达,应用免疫组化法检测B细胞淋巴瘤蛋白-2（Bcl-2）的表达。结果 miR-155-5p在不同肿瘤最大径、不同WHO分级分组的表达中差异有统计学意义（P<0.05）。生存分析发现,miR-155-5p高表达患者生存时间短（P<0.05）。miR-155-5p与Caspase-3呈负相关（P<0.05）。miR-155-5p与Bcl-2呈正相关（P<0.05）。结论胶质瘤中miR-155-5p表达升高,参与病变的形成和进展。miR-155-5p与凋亡相关,术后检测miR-155-5p的表达对判断预后有一定价值。

关键词: 胶质瘤, miR-155-5p, 凋亡, 预后

CLC Number:

R739.41

CHEN Hao. Expression of miR-155-5p and its Relationship with Apoptosis Factors in Glioma[J]. Journal of Chengde Medical University, 2022, 39(5): 380-384.

陈昊. 胶质瘤中miR-155-5p的表达特征及与凋亡相关因子的相关性[J]. 承德医学院学报, 2022, 39(5): 380-384.

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