Abstract: Objective To study the effect of lncRNA-AK093407 (AK093407 for short) on colorectal cancer HCT-15 cells and related mechanisms. Methods Lv5 lentivirus overexpressed AK093047, and transfected siRNA to silence AK093407; qRT-PCR was used to identify the expression of AK093407; MTT and plate colony formation assays were used to detect cell proliferation; flow cytometry was used to detect cell cycle and apoptosis; wound healing assay and transwell was used to detect cell invasion and migration; qRT-PCR and Western blot were used to detect the expression of EMT-related factors. Results The expression of AK093407 increased after Lv5 lentivirus infection. Overexpression of AK093407 in colorectal cancer cells increased proliferation activity, promoted cell cycle evolution, decreased apoptosis, and enhanced migration and invasion ability. The results of qRT-PCR and Western blotting showed that the transcription and translation levels of occludin and E-cadherin were decreased (P<0.05), while β-catenin, vimentin and fibronectin were increased (P<0.05). After the AK093407 gene was silenced, the above results were opposite. Conclusion AK093407 promotes colorectal cancer cell proliferation, cell cycle, invasion and migration and inhibits apoptosis via promoting EMT.

Key words: colorectal cancer, lnc-AK093407, cell cycle, invasion, metastasis

摘要： 目的 研究AK093407对结直肠癌HCT-15细胞的影响及相关机制。方法 Lv5慢病毒感染过表达AK093047,转染siRNA沉默AK093407;qRT-PCR鉴定AK093407的表达;MTT和平板克隆形成实验检测细胞增殖;流式细胞术检测细胞周期和凋亡;划痕修复实验和Transwell检测细胞侵袭和迁移;qRT-PCR和蛋白印记检测EMT相关因子。结果 Lv5慢病毒感染后,AK093407表达升高。过表达AK093407结直肠癌细胞增殖活性升高,促进细胞周期演进,凋亡降低,迁移和侵袭能力增强。qRT-PCR和蛋白印记结果显示,occludin和E-cadherin的转录和翻译水平降低（P<0.05）;β-catenin、vimentin和fibronectin的转录和翻译水平升高（P<0.05）。AK093407沉默后,与上述结果相反。结论 AK093407通过EMT促进结直肠癌细胞增殖、细胞周期、侵袭和迁移并抑制细胞凋亡。

关键词: 结直肠癌, lnc-AK093407, 细胞周期, 侵袭, 转移