Abstract: Objective To analyze the changes of PRDM14 and IGFBP2 levels and their relationship with microvascular density in patients with pancreatic cancer before and after radical surgical resection. Methods Choose from January 2017 to December 2023 in Yanbian University Hospital during radical surgical resection surgery, 100 cases of pancreatic cancer patients as the research object, using flow cytometry instrument testing before and after operation in patients with lymphocytes,Enzyme-linked immunosorbent assay was used to detect the levels of S100A9, MMP-9, PRDM14 and IGFBP2 before and after surgery. Pearson correlation analysis was used to analyze the relationship between PRDM14, IGFBP2 and pancreatic cancer and microvessel density. ROC curve was applied to analyze the predictive value of PRDM14 and IGFBP2 for microvascular density in patients with pancreatic cancer. Results After radical surgical resection, the levels of S100A9, MMP-9, PRDM14 and IGFBP2 were decreased, while the levels of lymphocytes were significantly increased (P<0.05). The microvascular density was not related to sex, age and tumor location, but was related to tumor diameter, differentiation degree, TNM stage and lymph node metastasis. The microvascular density in the low-differentiated group was significantly higher than that in the medium-differentiated group and the highly differentiated group, and the microvascular density in the medium-differentiated group was higher than that in the highly differentiated group. The microvascular density of lymph node metastasis group was significantly higher than that of non-lymph node metastasis group (P<0.05). Spearman correlation analysis showed that the expression of PRDM14 and IGFBP2 were positively correlated with microvascular density in pancreatic cancer patients, with statistical differences (P<0.05). ROC analysis showed that combined diagnosis was higher than single diagnosis of PRDM14 and IGFBP2, with statistical difference (P<0.05). Conclusion The levels of PRDM14 and IGFBP2 showed a decreasing trend after radical surgical resection, and the levels of PRDM14 and IGFBP2 were positively correlated with microvascular density.

Key words: Pancreatic cancer, Radical surgical resection, Positive regulatory zone zinc finger protein 14, Insulin-like growth factor binding protein 2, Microvessel density

摘要： 目的 分析胰腺癌患者进行根治性外科切除手术前和手术后PRDM14、IGFBP2水平变化及与微血管密度的关系。方法 选取2017年1月~2023年12月期间在延边大学附属医院进行根治性外科切除手术的100例胰腺癌患者作为研究对象,使用流式细胞仪检测患者手术前和手术后淋巴细胞,酶联免疫吸附法检测患者手术前和手术后S100A9、MMP-9、PRDM14、IGFBP2水平,Pearson相关性分析PRDM14、IGFBP2指标与胰腺癌的相关性与微血管密度的关系。ROC曲线分析PRDM14、IGFBP2对胰腺癌患者微血管密度的预测价值。结果 胰腺癌患者在进行根治性外科切除手术后,其S100A9、MMP-9、PRDM14、IGFBP2水平呈降低趋势,淋巴细胞水平显著升高（P<0.05）;微血管密度与性别、年龄和肿瘤部位无关,与肿瘤直径、分化程度、TNM分期、淋巴结转移相关,其中,低分化组的微血管密度显著高于中分化组和高分化组,中分化组的微血管密度比高分化组高;淋巴结转移组的微血管密度显著高于淋巴结未转移组,具有统计学差异（P<0.05）。Spearman相关性分析显示,PRDM14、IGFBP2表达与胰腺癌患者微血管密度均呈正相关,具有统计学差异（P<0.05）。ROC分析显示,联合诊断高于PRDM14、IGFBP2单项诊断,具有统计学差异（P<0.05）。结论 PRDM14、IGFBP2水平在胰腺癌患者行根治性外科切除手术后呈降低趋势,PRDM14、IGFBP2水平与微血管密度呈正相关。

关键词: 胰腺癌, 根治性外科切除手术, 正性调节区锌指蛋白14, 胰岛素样生长因子结合蛋白2, 微血管密度