Knockdown of LncRNA TCONS-00022381 Regulates Apoptosis in Human Gastric Cancer MKN45 Cells

Abstract

Abstract: Objective To investigate the effect of knocking down LncRNA TCONS-00022381 on apoptosis of human gastric cancer cells MKN45. Methods Culturing Human gastric cancer MKN45 cells and dividing them into three groups. Cells in each group were transfected using the Liposome transfection method, the siRNA targrting LncRNA TCONS-00022381 was transfected into the si-TCONS group, the negative siRNA was transfected into the NC group, and the Blank group was cultured in routine. The cell viability of each group was measured after 24h, 48h, and 72h of transfection using the CCK8 assay TCONS knockdown efficiency was detected by RTqPCR after 24h of transfection. The expression of Bcl-2 and Bax was detected by western blotting after 48h hours of transfection. The apoptotic rate of cells was detected by flow cytometry after 48h of transfection. Results The siRNA targeting LncRNA TCONS-00022381 significantly inhibited its expression in MKN45 cells (P<0.001). Compared with the Blank group and the NC group, the viability of MKN45 cell in the si-TCONS group decreased significantly (P<0.001). The expression of Bcl-2 was significantly down-regulated (P<0.01). The expression of Bax was significantly up-regulated (P<0.01). The rate of cell apoptosis increased significantly (P<0.05). Conclusion Knock down of LncRNA TCONS-00022381 may promote the apoptosis of MKN45 cells by regulating the expression of Bcl-2 and Bax.

Key words: Gastric cancer, LncRNA TCONS-00022381, MKN45 cells, Apoptosis

摘要： 目的探究敲低LncRNA TCONS-00022381对人胃癌MKN45细胞凋亡的影响。方法培养人胃癌MKN45细胞并将细胞分为3组,采用脂质体转染法转染各组细胞,将靶向LncRNA TCONS-00022381的siRNA转染于si-TCONS组细胞,阴性siRNA转染于NC组,Blank组按常规培养。用CCK8法测定转染24 h、48 h和72 h后各组细胞的活力;转染24 h后用RT-qPCR法检测TCONS的敲低效率;转染48 h后应用Western blotting检测各组细胞Bcl-2和Bax的表达;转染48 h后流式细胞仪检测各组细胞的细胞凋亡率。结果靶向LncRNA TCONS-00022381的siRNA显著抑制其在MKN45细胞中的表达（P<0.001）;与Blank组和NC组相比,si-TCONS组MKN45细胞的活力明显下降（P<0.001）;Bcl-2的表达显著下调（P<0.01）;Bax的表达显著上调（P<0.01）;细胞凋亡率明显增加（P<0.05）。结论敲低LncRNA TCONS-00022381可能通过调控Bcl-2和Bax的表达来促进胃癌MKN45细胞的凋亡。

关键词: 胃癌, LncRNA TCONS-00022381, MKN45细胞, 凋亡

CLC Number:

R735.2

LI Yun-dan, BAI You-lan, JIN Yu-ting, LI Xin. Knockdown of LncRNA TCONS-00022381 Regulates Apoptosis in Human Gastric Cancer MKN45 Cells[J]. Journal of Chengde Medical University, 2025, 42(2): 91-96.

李云丹, 柏友兰, 晋雨婷, 李欣. 敲低LncRNA TCONS-00022381对人胃癌MKN45细胞凋亡的影响[J]. 承德医学院学报, 2025, 42(2): 91-96.

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http://tougao.cdmc.edu.cn:8088/EN/Y2025/V42/I2/91

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