REGULATING EFFECTS OF INTRAVENTRICULAR INJECTION OF LEPTIN ON MMP-7 PROTEIN IN UTERINE FLUID OF OEP RATS

Journal of Chengde Medical University ›› 2019, Vol. 36 ›› Issue (1): 1-4.

REGULATING EFFECTS OF INTRAVENTRICULAR INJECTION OF LEPTIN ON MMP-7 PROTEIN IN UTERINE FLUID OF OEP RATS

School of Basic Medical Science, Chengde Medical College, Hebei Chengde 067000, China

Received:2018-09-25 Online:2019-02-10 Published:2021-11-18

侧脑室注射瘦素对OEP大鼠子宫腔液MMP-7蛋白的调节作用^*

司丽娜¹, 陈磊¹, 魏萌¹, 乔跃兵², 程露阳¹, 杨松鹤^1△

1.承德医学院基础医学院,河北承德 067000;
2.沧州医学高等专科学校

通讯作者: △
基金资助:
* 河北省自然科学基金资助项目（H2013406115）; 河北省科技厅计划项目（08276101D-20）; 河北省高等学校科学研究项目（QN2015121）; 河北省高校重点发展学科“人体解剖与组织胚胎学”建设项目资助

Abstract

Abstract: Objective: To observe the effects of intraventricular microinjection of leptin on matrix metalloproteinases-7 (MMP-7) in uterine fluid of OEP rats. Methods: OEP rats model was divided into leptin injection group and normal saline injection group with 8 rats in each group. 4μl leptin and 4μl normal saline were injected into the lateral ventricle of rats in the 2 groups respectively; and 6 hours after injection, the uterine fluid of rats were obtained. Two-dimensional gel electrophoresis and MALDI-TOF-MS were used to separate and identify the differential proteins in uterine fluid; At last, Western blot was used to compare the MMP-7 expression in uterine fluid of rats in leptin injection group and normal saline injection group to verify the results of MALDI-TOF-MS. Results: The differential target protein separating by two-dimensional gel electrophoresis was MMP-7 identified by MALDI-TOF-MS. Western blot showed that the MMP-7 expression in uterine fluid of rats in leptin injection group was obviously higher than normal saline injection group (P<0.05). Conclusions: Microinjection of leptin into lateral ventricle can positively regulate the MMP-7 protein expression in uterine fluid of OEP rats for a certain amount of time.

Key words: Leptin, OEP rat, Uterine fluid, Matrix metalloproteinases-7 (MMP-7)

摘要： 目的:观察侧脑室微量注射瘦素（leptin）对雌性OEP大鼠子宫腔液基质金属蛋白酶-7（MMP-7）的影响。方法:建立卵巢摘除给予雌激素大鼠（OEP）模型,随机分为瘦素注射组（大鼠侧脑室注射瘦素4μl）及生理盐水注射组（大鼠侧脑室注射生理盐水4μl）,每组8只大鼠。侧脑室微量注射6h后,提取两组大鼠子宫腔液;采用双向凝胶电泳（2-DE）法分离子宫腔液蛋白,并对其中差异表达蛋白进行基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术鉴定;最后采用蛋白印迹法比较两组大鼠子宫腔液MMP-7蛋白的表达情况,验证MALDI-TOF-MS的结果。结果:质谱法鉴定经双向凝胶电泳分离的差异目标蛋白为MMP-7;蛋白印迹法显示,瘦素注射组OEP大鼠子宫腔液MMP-7蛋白的表达明显高于生理盐水注射组（P<0.05）。结论:侧脑室微量注射瘦素可在一定时间内对OEP大鼠子宫腔液MMP-7蛋白的表达起到正向调节的作用。

关键词: 瘦素, OEP大鼠, 子宫腔液, 基质金属蛋白酶7（MMP-7）

CLC Number:

R339.2

SI Li-na, CHEN Lei, WEI Meng, et al. REGULATING EFFECTS OF INTRAVENTRICULAR INJECTION OF LEPTIN ON MMP-7 PROTEIN IN UTERINE FLUID OF OEP RATS[J]. Journal of Chengde Medical University, 2019, 36(1): 1-4.

司丽娜, 陈磊, 魏萌, 乔跃兵, 程露阳, 杨松鹤. 侧脑室注射瘦素对OEP大鼠子宫腔液MMP-7蛋白的调节作用^*[J]. 承德医学院学报, 2019, 36(1): 1-4.

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